Difference between revisions of "Os12g0277500"
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* When leaf chlorophyll contents were measured, the researchers found that both chlorophyll a and b were severely decreased in the mutant, to approximately 37% of that recorded from the WT siblings (Fig. 3E). | * When leaf chlorophyll contents were measured, the researchers found that both chlorophyll a and b were severely decreased in the mutant, to approximately 37% of that recorded from the WT siblings (Fig. 3E). | ||
* That is, after the fourth leaf emerged from an oscpn60α1 plant, no further leaves were produced, resulting in a lethal character. TEM analyses showed that chloroplast development was impaired in the mutant (Fig.3F). | * That is, after the fourth leaf emerged from an oscpn60α1 plant, no further leaves were produced, resulting in a lethal character. TEM analyses showed that chloroplast development was impaired in the mutant (Fig.3F). | ||
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[[File:146-Os12g0277500.png|center|thumb|727px|'''Figure 3.''' ''Phenotypes of oscpn60α1 mutant.<ref name="ref1" />.'']] | [[File:146-Os12g0277500.png|center|thumb|727px|'''Figure 3.''' ''Phenotypes of oscpn60α1 mutant.<ref name="ref1" />.'']] | ||
Latest revision as of 15:16, 20 October 2016
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Contents
Annotated Information
Function
- OsCpn60α1 encodes Plastid Chaperonin 60α Subunit.
- OsCpn60α1 is essential for folding of rbcL.
- OsCpn60α1 is required for the folding of rbcL and that failure of that process is seedling-lethal.
Phenotypic analysis
- Homozygous mutant seedlings were pale green (Figs. 3C and 3D). In progeny of the OsCpn60α1/oscpn60α1 heterozygote, that phenotype was segregated in an approximately 3:1 ratio (WT:oscpn60α1); all pale-green seedlings were oscpn60α1 homozygous plants. This indicated that the mutant phenotype was recessive and was caused by a defect in a single genomic locus.
- When leaf chlorophyll contents were measured, the researchers found that both chlorophyll a and b were severely decreased in the mutant, to approximately 37% of that recorded from the WT siblings (Fig. 3E).
- That is, after the fourth leaf emerged from an oscpn60α1 plant, no further leaves were produced, resulting in a lethal character. TEM analyses showed that chloroplast development was impaired in the mutant (Fig.3F).
Figure 3. Phenotypes of oscpn60α1 mutant.[1].
Subcellular localization
- Subcellular localization analysis showed that transiently expressed OsCpn60α1:GFP fusion protein was localized to the chloroplast stroma.
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Labs working on this gene
- Crop Biotech Institute and Department of Genetic Engineering, Kyung Hee University, Yongin 446-701, Korea
References
- ↑ Kim SR, Yang JI, An G. OsCpn60α1, encoding the plastid chaperonin 60α subunit, is essential for folding of rbcL. Mol Cells. 2013 May;35(5):402-9. doi: 10.1007/s10059-013-2337-2. Epub 2013 Apr 24. PubMed PMID: 23620301; PubMed Central PMCID: PMC3887859.
