Difference between revisions of "Os08g0445700"
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==Annotated Information== | ==Annotated Information== | ||
===Function=== | ===Function=== | ||
| − | + | * Trehalose-6-phosphate synthase (TPS) is a key enzyme for trehalose biosynthesis. | |
| − | Trehalose-6-phosphate synthase (TPS) is a key enzyme for trehalose biosynthesis.Trehalose plays an important role in metabolic regulation and abiotic stress tolerance in a variety of organisms. In plants, its biosynthesis is catalyzed by two key enzymes: trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP). The genome of rice (Oryza sativa) contains 11 OsTPS genes, and only OsTPS1 shows TPS activity. | + | * Trehalose plays an important role in metabolic regulation and abiotic stress tolerance in a variety of organisms. |
| + | * In plants, its biosynthesis is catalyzed by two key enzymes: trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP). The genome of rice (Oryza sativa) contains 11 OsTPS genes, and only OsTPS1 shows TPS activity. | ||
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| + | ===Phenotypic analysis=== | ||
| + | * Leaves of OsTPS1 knockout (KO) plants were more sensitive to drought or cold stress than were the wild types. | ||
| + | * Furthermore, transgenic rice of UBI::CBF1 had high expression OsTPS1 mRNA, suggesting that '''''OsTPS1''''' is regulated by the CBF/DREB transcription factor. | ||
| + | * '''''OsTPS1''''' overexpression improved the tolerance of rice seedling to cold, high salinity and drought treatments without other significant phenotypic changes | ||
| + | |||
===Expression=== | ===Expression=== | ||
| − | + | * Reverse transcriptase-PCR analysis of OsTPS1 showed that OsTPS1 is inducible by drought, salt, cold, and ABA. | |
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You can also add sub-section(s) at will. | You can also add sub-section(s) at will. | ||
Revision as of 01:04, 27 March 2017
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Contents
Annotated Information
Function
- Trehalose-6-phosphate synthase (TPS) is a key enzyme for trehalose biosynthesis.
- Trehalose plays an important role in metabolic regulation and abiotic stress tolerance in a variety of organisms.
- In plants, its biosynthesis is catalyzed by two key enzymes: trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP). The genome of rice (Oryza sativa) contains 11 OsTPS genes, and only OsTPS1 shows TPS activity.
Phenotypic analysis
- Leaves of OsTPS1 knockout (KO) plants were more sensitive to drought or cold stress than were the wild types.
- Furthermore, transgenic rice of UBI::CBF1 had high expression OsTPS1 mRNA, suggesting that OsTPS1 is regulated by the CBF/DREB transcription factor.
- OsTPS1 overexpression improved the tolerance of rice seedling to cold, high salinity and drought treatments without other significant phenotypic changes
Expression
- Reverse transcriptase-PCR analysis of OsTPS1 showed that OsTPS1 is inducible by drought, salt, cold, and ABA.
You can also add sub-section(s) at will.
Labs working on this gene
To understand the function of the TPS gene, we identified the T-DNA insertion in a TPSgene of rice.<fil.1>
To demonstrate the physiological function of OsTPS1, we introduced it into rice and found that OsTPS1 overexpression improved the tolerance of rice seedling to cold, high salinity and drought treatments without other significant phenotypic changes.<fil.2>
References
1. Baisheng Zang;Haowen Li;Wenjun Li;Xing Wang Deng;Xiping Wang
Analysis of trehalose-6-phosphate synthase (TPS) gene family suggests the formation of TPS complexes in rice Plant Molecular Biology, 2011, 76(6): 507-522
2. Hao-Wen Li;Bai-Sheng Zang;Xing-Wang Deng;Xi-Ping Wang
Overexpression of the trehalose-6-phosphate synthase gene OsTPS1 enhances abiotic stress tolerance in rice Planta, 2011, 234(5): 1007-1018
3. Richard A. Wilson;Robert P. Gibson;Cristian F. Quispe;Jennifer A. Littlechild;Nicholas J. Talbot
An NADPH-dependent genetic switch regulates plant infection by the rice blast fungus Proceedings of the National Academy of Sciences, 2010, 107(50): 21902-21907
4. Soo-Jin Kim;Dong-Hoon Jeong;Gynheung An;Seong-Ryong Kim
Characterization of a drought-responsive gene, OsTPS1 , identified by the T-DNA Gene-Trap system in rice Journal of Plant Biology, 2005, 48(4): 371-379
