Difference between revisions of "Os03g0237200"
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| − | + | The rice '''''Os03g0237200''''' was reported as '''''LPA1''''' in 2015 <ref name="ref1" /> by researchers from Korea and Japan. | |
==Annotated Information== | ==Annotated Information== | ||
===Function=== | ===Function=== | ||
| − | + | * Loose Plant Architecture1 (LPA1) determines lamina joint bending by suppressing auxin signalling that interacts with C-22-hydroxylated and 6-deoxo brassinosteroids in rice. | |
| + | |||
| + | ===Phenotypic analysis=== | ||
| + | * The researchers examined the mode of action of LPA1 on lamina bending by comparing the phenotypes among knockout, overexpression and repressor-fusion transgenic plants. | ||
| + | * A strong synergic effect is detected between lpa1 and d2 (the defective mutant for catalysis of C-23-hydroxylated BRs) during IAA-mediated lamina inclination. | ||
===Expression=== | ===Expression=== | ||
| − | + | * The Ubiquitin promoter was used to overexpress LPA1 cDNA. To construct a vector expressing repressor-fused LPA1, the C-terminus of LPA1 was fused with the 24 amino acid fragment containing a conserved ERF-associated amphiphilic repression (EAR) motif of a gene (LOC_Os02g01090) orthologous to Arabidopsis SUPERMAN (At3g23130). | |
| − | + | * RNA sequencing analysis and qRT-PCR indicate that LPA1 influences the expression of three OsPIN genes (OsPIN1a, OsPIN1c and OsPIN3a), which suggests that auxin flux might be an important factor in LPA1-mediated lamina inclination in rice. | |
===Evolution=== | ===Evolution=== | ||
Please input evolution information here. | Please input evolution information here. | ||
Revision as of 14:44, 17 January 2018
The rice Os03g0237200 was reported as LPA1 in 2015 [1] by researchers from Korea and Japan.
Contents
Annotated Information
Function
- Loose Plant Architecture1 (LPA1) determines lamina joint bending by suppressing auxin signalling that interacts with C-22-hydroxylated and 6-deoxo brassinosteroids in rice.
Phenotypic analysis
- The researchers examined the mode of action of LPA1 on lamina bending by comparing the phenotypes among knockout, overexpression and repressor-fusion transgenic plants.
- A strong synergic effect is detected between lpa1 and d2 (the defective mutant for catalysis of C-23-hydroxylated BRs) during IAA-mediated lamina inclination.
Expression
- The Ubiquitin promoter was used to overexpress LPA1 cDNA. To construct a vector expressing repressor-fused LPA1, the C-terminus of LPA1 was fused with the 24 amino acid fragment containing a conserved ERF-associated amphiphilic repression (EAR) motif of a gene (LOC_Os02g01090) orthologous to Arabidopsis SUPERMAN (At3g23130).
- RNA sequencing analysis and qRT-PCR indicate that LPA1 influences the expression of three OsPIN genes (OsPIN1a, OsPIN1c and OsPIN3a), which suggests that auxin flux might be an important factor in LPA1-mediated lamina inclination in rice.
Evolution
Please input evolution information here.
You can also add sub-section(s) at will.
Labs working on this gene
Please input related labs here.
References
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Structured Information
- ↑ Cite error: Invalid
<ref>tag; no text was provided for refs namedref1
