Difference between revisions of "Os04g0607600"
Xiaonan liu (talk | contribs) |
Xiaonan liu (talk | contribs) |
||
| Line 6: | Line 6: | ||
===Function=== | ===Function=== | ||
* OsHKT1;4-mediated Na+ transport in stems contributes to Na+ exclusion from leaf blades of rice at the reproductive growth stage upon salt stress. | * OsHKT1;4-mediated Na+ transport in stems contributes to Na+ exclusion from leaf blades of rice at the reproductive growth stage upon salt stress. | ||
| + | |||
===Expression=== | ===Expression=== | ||
* Heterologous expression analysis was performed using a salt hypersensitive mutant of S. cerevisiae (strain G19). | * Heterologous expression analysis was performed using a salt hypersensitive mutant of S. cerevisiae (strain G19). | ||
| Line 12: | Line 13: | ||
===Phenotypic analysis=== | ===Phenotypic analysis=== | ||
| − | * phenotypic analysis of OsHKT1;4 RNAi plants in the vegetative growth stage revealed no profound influence on the growth and ion accumulation in comparison with WT plants upon salinity stress. | + | * phenotypic analysis of OsHKT1;4 RNAi plants in the vegetative growth stage revealed no profound influence on the growth and ion accumulation in comparison with WT plants upon salinity stress. |
| − | === | + | |
| + | ===Subcellular localization=== | ||
* To determine the localization of the OsHKT1;4 protein in plant cells, we fused EGFP at the N-terminus end of OsHKT1;4 (EGFP-OsHKT1;4 was shown to be functional in X. laevis oocytes) and placed under the control of the CaMV35S promoter.The result strongly indicated that EGFP-OsHKT1;4 localizes to the PM of rice protoplasts. However, by repeating the transformation experiments several times, we often observed that EGFP-OsHKT1;4 was also present inside the cells and clustered in punctate-like structures. | * To determine the localization of the OsHKT1;4 protein in plant cells, we fused EGFP at the N-terminus end of OsHKT1;4 (EGFP-OsHKT1;4 was shown to be functional in X. laevis oocytes) and placed under the control of the CaMV35S promoter.The result strongly indicated that EGFP-OsHKT1;4 localizes to the PM of rice protoplasts. However, by repeating the transformation experiments several times, we often observed that EGFP-OsHKT1;4 was also present inside the cells and clustered in punctate-like structures. | ||
Latest revision as of 03:12, 19 January 2018
The rice Os04g0607600 was reported as HKT1;4 in 2016 [1] by researchers from Japan, Italy and USA.
Contents
Annotated Information
Gene Symbol
- Os04g0459000 <=> OsHKT7,HKT7
Function
- OsHKT1;4-mediated Na+ transport in stems contributes to Na+ exclusion from leaf blades of rice at the reproductive growth stage upon salt stress.
Expression
- Heterologous expression analysis was performed using a salt hypersensitive mutant of S. cerevisiae (strain G19).
- OsHKT1;4 expression increases Na+ hypersensitivity of yeast cells promoting Na+ over-accumulation. The OsHKT1;4 cDNA from rice cultivar Nipponbare was constitutively expressed in strain G19 of S. cerevisiae under the control of the GAP promoter.
- G19 cells harboring the empty vector or expressing OsHKT1;4 were grown on the arginine phosphate (AP) medium containing 1 mM KCl with or without the addition of 50 mM NaCl.
Phenotypic analysis
- phenotypic analysis of OsHKT1;4 RNAi plants in the vegetative growth stage revealed no profound influence on the growth and ion accumulation in comparison with WT plants upon salinity stress.
Subcellular localization
- To determine the localization of the OsHKT1;4 protein in plant cells, we fused EGFP at the N-terminus end of OsHKT1;4 (EGFP-OsHKT1;4 was shown to be functional in X. laevis oocytes) and placed under the control of the CaMV35S promoter.The result strongly indicated that EGFP-OsHKT1;4 localizes to the PM of rice protoplasts. However, by repeating the transformation experiments several times, we often observed that EGFP-OsHKT1;4 was also present inside the cells and clustered in punctate-like structures.
You can also add sub-section(s) at will.
Labs working on this gene
- State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China;
- Department of Biology - Plant Biology, University of Fribourg, Rue Albert-Gockel 3, CH-1700 Fribourg, Switzerland
- State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
- State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China
- State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
References
- ↑ Suzuki K, Yamaji N, Costa A, Okuma E, Kobayashi NI, Kashiwagi T, Katsuhara M, Wang C, Tanoi K, Murata Y, Schroeder JI, Ma JF, Horie T. OsHKT1;4-mediated Na(+) transport in stems contributes to Na(+) exclusion from leaf blades of rice at the reproductive growth stage upon salt stress. BMC Plant Biol. 2016 Jan 19;16:22. doi: 10.1186/s12870-016-0709-4. PubMed PMID: 26786707; PubMed Central PMCID: PMC4719677.
