Os08g0445700

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Annotated Information

Function

• Trehalose-6-phosphate synthase (TPS) is a key enzyme for trehalose biosynthesis.
• Trehalose plays an important role in metabolic regulation and abiotic stress tolerance in a variety of organisms.
• In plants, its biosynthesis is catalyzed by two key enzymes: trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP). The genome of rice (Oryza sativa) contains 11 OsTPS genes, and only OsTPS1 shows TPS activity.

Phenotypic analysis

• Leaves of OsTPS1 knockout (KO) plants were more sensitive to drought or cold stress than were the wild types.
• Furthermore, transgenic rice of UBI::CBF1 had high expression OsTPS1 mRNA, suggesting that OsTPS1 is regulated by the CBF/DREB transcription factor.
• OsTPS1 overexpression improved the tolerance of rice seedling to cold, high salinity and drought treatments without other significant phenotypic changes

Expression

• Reverse transcriptase-PCR analysis of OsTPS1 showed that OsTPS1 is inducible by drought, salt, cold, and ABA.

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Labs working on this gene

To understand the function of the TPS gene, we identified the T-DNA insertion in a TPSgene of rice.<fil.1>

To demonstrate the physiological function of OsTPS1, we introduced it into rice and found that OsTPS1 overexpression improved the tolerance of rice seedling to cold, high salinity and drought treatments without other significant phenotypic changes.<fil.2>

References

1. Baisheng Zang;Haowen Li;Wenjun Li;Xing Wang Deng;Xiping Wang

 Analysis of trehalose-6-phosphate synthase (TPS) gene family suggests the formation of TPS complexes in rice
 Plant Molecular Biology, 2011, 76(6): 507-522

2. Hao-Wen Li;Bai-Sheng Zang;Xing-Wang Deng;Xi-Ping Wang

 Overexpression of the trehalose-6-phosphate synthase gene OsTPS1 enhances abiotic stress tolerance in rice
 Planta, 2011, 234(5): 1007-1018

3. Richard A. Wilson;Robert P. Gibson;Cristian F. Quispe;Jennifer A. Littlechild;Nicholas J. Talbot

 An NADPH-dependent genetic switch regulates plant infection by the rice blast fungus
 Proceedings of the National Academy of Sciences, 2010, 107(50): 21902-21907

4. Soo-Jin Kim;Dong-Hoon Jeong;Gynheung An;Seong-Ryong Kim

 Characterization of a drought-responsive gene, OsTPS1 , identified by the T-DNA Gene-Trap system in rice
 Journal of Plant Biology, 2005, 48(4): 371-379