Os10g0558900
Please input one-sentence summary here.
Contents
Annotated Information
Function
Expression
Histochemistry, as well as qRT-PCR analysis, showed that RL14 was highly transcribed in leaves, leaf sheaths and roots. It was also detected in stems and glumes, although its expression in these organs was not as intense as that in the mature leaves, leaf sheaths and roots (Figure 1b). Expression of RL14 mRNA was detected in the sclerenchymal cell layer of underemerged leaves and sheaths. In the mature leaf, RL14 mRNA accumulated mainly in the mesophyll cells that surrounded the vasculature (Figure 1c).
Fig 1.Analysis of the expression pattern for the RL14 gene
Evolution
The RL14 gene was mapped first to an interval between the simple sequence repeat (SSR) markers RM3123 and RM1162 on the long arm of chromosome 10. Finally, the RL14 locus was mapped to a 24.5-kb DNA region between the InDel marker SID2 and SSR marker SW24 on a single bacterial artificial chromosome, AC079874. Four annotated candidate genes, which encoded a polyol transporter (Os10g40950.1), a 2OG-Fe (II) oxygenase family protein (Os10g40960.1), a flavonol synthase (Os10g40990.1) and a retrotransposon protein (Os10g41000), were located in the 24.5-kb DNA region. RL14 was related most closely to members of the 2OG-Fe (II) oxygenase family from rice and sorghum (Figure 2b). Orthologous of RL14 was identified in other species, such as maize, Arabidopsis, Papaver somniferum, Vitis spp and Ricinus communis (Figure 2b).
Fig 2.Phylogenetic analysis and subcellular localization of RL14 protein
Labs working on this gene
Rice Research Institute, Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Southwest University, Chongqing, China.
References
Likui Fang,Fangming Zhao,Yunfei Cong,et al.Rolling-leaf14 is a 2OG-Fe (II) oxygenase family protein that modulates rice leaf rolling by affecting secondary cell wall formation in leaves.Plant Biotechnology Journal, 2012, 10(5): 524-532.
Structured Information
| Gene Name |
Os10g0558900 |
|---|---|
| Description |
2OG-Fe(II) oxygenase domain containing protein |
| Version |
NM_001071899.1 GI:115483393 GeneID:4349365 |
| Length |
2021 bp |
| Definition |
Oryza sativa Japonica Group Os10g0558900, complete gene. |
| Source |
Oryza sativa Japonica Group ORGANISM Oryza sativa Japonica Group
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
clade; Ehrhartoideae; Oryzeae; Oryza.
|
| Chromosome | |
| Location |
Chromosome 10:22450841..22452861 |
| Sequence Coding Region |
22451155..22451400,22452093..22452404 |
| Expression | |
| Genome Context |
<gbrowseImage1> name=NC_008403:22450841..22452861 source=RiceChromosome10 preset=GeneLocation </gbrowseImage1> |
| Gene Structure |
<gbrowseImage2> name=NC_008403:22450841..22452861 source=RiceChromosome10 preset=GeneLocation </gbrowseImage2> |
| Coding Sequence |
<cdnaseq>tgcatactcatcagagacagaaaatattggactctgcctgctgcaattcatgccaaggccgttggtgttgagccgaagtcgttgttaagtgtatttgaagggcaagctaggggtttgaggatgaactactatccaccgtgcttgaaagctgacaaggtgttgggtttgtcaccgcacactgatccaggtggcttgacactgcttctccaggtgaatgatgtacagggcctgcagatcaacaaggatggcaaatggttctccgtgaatgctctgaatggtgcactcatcgttaacattggcgacacacttgagatcctgagtaatggaaagttcagaagtgttgagcacagggccgtggtacatccaagcagagagaggatttcagcggcactgttctactatccgtgccaggatatggtgatcagccctctgccggactttgtgaaagatggtaaagtgaaatacaaaacaataagctaccaggatttgctgacagagtacttcacagcagagcttgatggaaggaaccggctaaagaaaatgaagctggaaccgtag</cdnaseq> |
| Protein Sequence |
<aaseq>CILIRDRKYWTLPAAIHAKAVGVEPKSLLSVFEGQARGLRMNYY PPCLKADKVLGLSPHTDPGGLTLLLQVNDVQGLQINKDGKWFSVNALNGALIVNIGDT LEILSNGKFRSVEHRAVVHPSRERISAALFYYPCQDMVISPLPDFVKDGKVKYKTISY QDLLTEYFTAELDGRNRLKKMKLEP</aaseq> |
| Gene Sequence |
<dnaseqindica>1462..1707#458..769#atgtactgacagtagctctagctcagtttgagatgtgcaaatagtataacaaaagcattacttatgcaacaaaaactacagctcatcaaccatggagtgccagatgaagtgattgcaaatctcaagaacgacttggttgagtttttcagtcagccactggatgccaagaaggaatactcacagctgcccaataaccttgaaggttatggacaggccttcgttgtgtccgacaaccagaaactagactgggcagacatgctataccttcaagtctgtcctaccgattcaagggacttgagattctggcccaattaccctgcctctttcaggtgatactaccgacaaattattatgcaactaattactccattgataagcatatgttcattataacttttaaagcatacatatgtccatttattaacttgaaatatctgaaaattaggcattccattgatgcatactcatcagagacagaaaatattggactctgcctgctgcaattcatgccaaggccgttggtgttgagccgaagtcgttgttaagtgtatttgaagggcaagctaggggtttgaggatgaactactatccaccgtgcttgaaagctgacaaggtgttgggtttgtcaccgcacactgatccaggtggcttgacactgcttctccaggtgaatgatgtacagggcctgcagatcaacaaggatggcaaatggttctccgtgaatgctctgaatggtgcactcatcgttaacattggcgacacacttgaggtaatgactaatcccgttctcggggcaattgtatcgtatcgatgtaatttcatgagttaaataaagcttttatctttatactattataaaagttgaagatatttttcccggtattttggtgcgtcatccatgtatgagtcagtttctaagttcgttcgcttttggaaatacatatccgtatttgagtcggtttttaagatagttcacttttgataatacagaaggaataatataagaaatctgtttaaaaaactcgcatgctaacatgaggtgatcggactcctaactgcagctcatgattttctaaatatatatatatatatatatatatatatatatatatccaaacgaactcccacaataaatttcatcttaactaaaccatataacaataataatattaaaatagactccaccagttgcaacacacgagcattttttctagttatttaaaaaaaaagaactaatcccgtttgatataggatattaggcagattattttattgtgaatcaaaatatttttaagaaataaatttaacaaatagcttaaaacaaatgatcagtacaaaatcattttggttgtggccgataagccaatgagtagacaatcaaagccgctgatgctcaacttttggatcaacatgtttttctgatgtacaagtacggtcgatgtgcgctgttgatgtgtgcagatcctgagtaatggaaagttcagaagtgttgagcacagggccgtggtacatccaagcagagagaggatttcagcggcactgttctactatccgtgccaggatatggtgatcagccctctgccggactttgtgaaagatggtaaagtgaaatacaaaacaataagctaccaggatttgctgacagagtacttcacagcagagcttgatggaaggaaccggctaaagaaaatgaagctggaaccgtagccatcgaccatgatgcatgtaacaattttataagaagaacctgctttcaggttgtgtcctacttactgtatctgctatctactctcttcatcctaaaataaataaacatagtacatatatgatacatcatagtaccataaattagaaaatgttacatcgtgtactatgtttttttaaaaaatttgggatataggaaatatatgtcgtgaagaataatgtattcaggcatcaagctttatgcataattagcttatgcctgtaaactgcgttgaacatgttcagtttgatgaaacagacatggaagtttgcctctt</dnaseqindica> |
| External Link(s) |
