IC4R006-Proteomic-2007-17132620

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Project Title

  • Proteomics Identification of Differentially Expressed Proteins Associated with Pollen Germination and Tube Growth Reveals Characteristics of Germinated Oryza sativa Pollen

The Background of This Project

  • Mature pollen from most plant species is metabolically quiescent; however, after pollination, it germinates quickly and gives rise to a pollen tube to transport sperms into the embryo sac. Because methods for collecting a large amount of in vitro germinated pollen grains for transcriptomics and proteomics studies from model plants of Arabidopsis and rice are not available, molecular information about the germination developmental process is lacking. In this project, the researchers describe a method for obtaining a largequantity of in vitro germinating rice pollen for proteomics study.

Plant Culture & Treatment

Protein Extraction and 2-D PAGE

Research Findings

Labs working on this Project

  • Research Center for Molecular and Developmental Biology, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
  • Graduate School of Chinese Academy of Sciences, Beijing 100049, China
  • Key Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
  • �Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China

Corresponding Author

  • twang@ibcas.ac.cn