HiCapR Raw script for HiCapR paper

# Background

HiCapR is a new RNA proximity ligation based method to detect RNA-RNA interactions, integrating a hybridization-based capture of HIV sequence after library construction, resulting in a new method called **Hi**gh throughput **Cap**turing **R**NA interaction analysis (HiCapR). This advancement enabled the comprehensive capture and analysis of the complete HIV RNA genome using HiCapR.

The major bioinformatic pipeline is adopted from previous established tools:

data preprocessing and chimeric reads identification:https://github.com/gkudla/hyb and https://github.com/zany1983/simplifed_SPLASH

calculating base pairing score: https://github.com/gkudla/comrades


Here in this repostitary, we provide scripts regarding to our manuscript "Mapping HIV-1 RNA Structure, Homodimers, and Long-Range Interactions by HiCapR"


# Prerequisites

Linux operating system
Perl 5
R(3.5 and above)

R packages: data.table, dplyr, ggplot2,ggrepel, ggbreak, pheatmap,


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