HRA000963
(Controlled Access)
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To systematically explore the complete tumor ecosystem heterogeneity at a single-cell level in primary tumor and paired tumor thrombus. A total of 22 surgically resected tissue samples were collected from patients with advanced renal cell carcinoma, which including primary tumors (n=9), paired tumor thrombus (n=9) and adjacent renal tissues (n=4). Cell capture and cDNA synthesis were carried out in 10X Genomics platform, and then transcriptome sequencing was performed in NovaSeq 6000 platform after meeting the all quality control. |