| HRA002688
(Controlled Access)
|
In this study, by combining clinical data from 8 individuals, integrating single-cell RNA sequencing (scRNA-seq) and cell surface protein sequencing from 23,990 cells, we not only show four distinct immune phenotypes, but also demonstrate changes in cell population abundance, gene expression, developmental trajectory, transcriptomic regulation, and cell-cell signaling, following infection. MTB immune response was mediated primarily through host T cells, myeloid cells, and natural killer cells, rather than B cells. Specifically, MTB suppressed naive, cytotoxicity and memory functions of T cells, rather than the immunoregulation function. The results allow us to define MTB-host immune cell interactions and propose novel immunotherapeutic treatment strategies to restore T cells from dysfunctional or exhausted states. |
