| HRA006501
(Controlled Access)
|
We established organoid lines from two surgical tumor specimens of a BBC patient, with one line undergoing detailed pathological and genomic analysis. The derived BBC organoid mirrored the pathological features and breast cancer biomarkers of the original tumor (TNBC from the right breast). Whole Exome Sequencing (WES) revealed that the organoids' copy number variations (CNVs), single nucleotide polymorphisms (SNPs), and insertions/deletions (indels) were more closely aligned with their source tissue (TNBC) than other tumor tissues. Evolutionary analysis suggested that different tumor regions might evolve from a common ancestral layer. In this case, the development of BBC organoids indicated that simultaneous lesions with diverse molecular profiles shared a high degree of consistency in key tumor-driving mutations. |
