| HRA009507
(Controlled Access)
|
A total of 193 patients with CaOx kidney stones and 99 healthy volunteers were recruited from the First People's Hospital of Yulin and the Second Nanning People's Hospital between April and September 2022. The eligibility criteria for CaOx stone formers (CSF) included ages 20-70 years, a diagnosis of upper urinary tract stones confirmed by imaging (X-ray, ultrasound, or CT), and post-surgical stone composition analysis confirming CaOx as the primary component through infrared spectroscopy. Healthy controls (HC) were aged 20-70 years, with no history of urolithiasis, confirmed by urinary ultrasound. Stool samples were collected from all subjects in the morning while fasting. Metagenomic Sequencing: Fecal DNA from 291 stool samples (192 CaOx stone patients and 99 healthy controls) was extracted using a Magnetic Soil and Stool DNA Kit (TIANGEN, DP712) and sequenced on the MGI-tech DNBSEQ-T7 platform (150bp paired-end) |
