Introduction
We provide two ways for users to use the CCLHunter webserver, as follows:
  1. Users do not need to install any software from CCLHunter. Only Samtools, which is a fundamental tool widely used in NGS data analysis, is used in this way to extract hundreds of SNP-barcodes from the user's BAM file. CCLHunter accepts the extracted smaller BAM file as input to run the identification program.
  2. If users have already downloaded the CCLHunter command-line program, they can upload the output from the command-line and view additional information.Only the second way requires the user to download and run the CCLHunter command-line program on their local machine. This way is more useful for advanced users.
If you have not downloaded the command line program of CCLHunter, please run the following command to extract the key information in your BAM file and upload the output. We will run the authentication program of CCLHunter for you in our task queue.
We provide a small wrapper called CCLHunter_preprocess(usage can be found at documentation to help users align their raw data to reference, generating the BAM file needed by CCLHunter.
Step 1
Select the reference version and the prefix option.
Step 2
  1. the command line below and paste it to your command line.
  2. Change the input and output name and run the command.
echo 5:89820984-89820984 8:144239859-144239859 9:95284982-95284982 14:75574087-75574087 22:43206950-43206950 7:21640361-21640361 22:17630486-17630486 15:31342744-31342744 6:39041502-39041502 20:56137807-56137807 1:19415304-19415304 20:44506418-44506418 8:27255263-27255263 4:6303955-6303955 12:5021228-5021228 2:215820013-215820013 16:15811062-15811062 17:55183716-55183716 8:132982824-132982824 12:6926363-6926363 19:4852137-4852137 17:47000251-47000251 8:21862551-21862551 12:129278864-129278864 14:77926011-77926011 11:33065394-33065394 17:5284770-5284770 9:107457532-107457532 12:96057387-96057387 1:186050417-186050417 1:241907817-241907817 9:125289521-125289521 11:48286256-48286256 12:29648275-29648275 8:25708267-25708267 1:62713246-62713246 1:118583408-118583408 2:207169731-207169731 7:48081095-48081095 7:98782750-98782750 11:5364450-5364450 11:6520015-6520015 15:91543761-91543761 19:43519362-43519362 8:145059425-145059425 9:90503451-90503451 10:95542677-95542677 10:118620459-118620459 1:17657616-17657616 5:118861713-118861713 3:53125922-53125922 9:134351343-134351343 17:6902760-6902760 11:64882789-64882789 17:37054772-37054772 16:66420885-66420885 4:8616096-8616096 7:37953840-37953840 10:50901938-50901938 10:13653653-13653653 2:231042276-231042276 7:73280020-73280020 9:37974743-37974743 3:50153356-50153356 1:207074905-207074905 2:153405594-153405594 15:64962630-64962630 17:64025331-64025331 2:103095404-103095404 4:140810700-140810700 11:63072310-63072310 8:18258316-18258316 1:158533140-158533140 11:47701528-47701528 11:4869649-4869649 15:101109818-101109818 7:25194753-25194753 7:22202117-22202117 1:227172290-227172290 17:10450816-10450816 8:95690579-95690579 1:117656088-117656088 19:53349850-53349850 20:31466534-31466534 5:64273018-64273018 4:164466824-164466824 22:42416056-42416056 1:147119273-147119273 1:205801872-205801872 3:66419956-66419956 15:27772676-27772676 13:28239970-28239970 6:161137779-161137779 18:28898294-28898294 5:148408101-148408101 1:202304868-202304868 15:42149472-42149472 7:129664312-129664312 2:235950187-235950187 6:54735122-54735122 3:183211906-183211906 20:36841756-36841756 9:15784631-15784631 2:113309473-113309473 17:66364804-66364804 12:58010163-58010163 12:53091566-53091566 5:141336264-141336264 3:124351424-124351424 19:37482151-37482151 8:110455321-110455321 16:72156842-72156842 8:53586706-53586706 10:45430462-45430462 7:48450157-48450157 1:228412308-228412308 3:122056432-122056432 5:169310213-169310213 4:71396989-71396989 20:4880308-4880308 14:51237701-51237701 11:72946020-72946020 16:84203612-84203612 12:123200768-123200768 1:53676448-53676448 4:3039150-3039150 18:60027241-60027241 1:92177938-92177938 12:13716638-13716638 9:107360922-107360922 5:81549216-81549216 14:23549319-23549319 5:150723806-150723806 16:51173559-51173559 16:24202615-24202615 7:23296533-23296533 22:36591380-36591380 17:42398052-42398052 3:35779750-35779750 12:53242440-53242440 8:101586133-101586133 5:38955796-38955796 16:27974487-27974487 1:183085755-183085755 20:31622083-31622083 9:139839904-139839904 4:2341194-2341194 1:167825485-167825485 22:29755888-29755888 16:15818141-15818141 17:18855611-18855611 11:33631423-33631423 17:14005439-14005439 2:25377176-25377176 1:234529552-234529552 9:4564432-4564432 2:75113657-75113657 15:40590134-40590134 15:99478225-99478225 1:103352451-103352451 5:112162854-112162854 12:26784850-26784850 6:56484758-56484758 4:25005193-25005193 7:43917013-43917013 20:36989381-36989381 22:24898310-24898310 20:48259034-48259034 14:64908845-64908845 6:36260858-36260858 19:5455611-5455611 2:208989037-208989037 21:31709691-31709691 3:100617680-100617680 9:130206762-130206762 18:28734749-28734749 4:187534375-187534375 5:147286054-147286054 3:53700550-53700550 20:44184375-44184375 6:152464839-152464839 4:2744087-2744087 10:50184949-50184949 5:149907533-149907533 14:31582526-31582526 1:45973928-45973928 11:76917220-76917220 18:31320229-31320229 15:78390414-78390414 17:6515454-6515454 2:33447202-33447202 12:21331625-21331625 17:80863857-80863857 2:48921375-48921375 8:124696867-124696867 14:23374435-23374435 1:207851611-207851611 20:52786219-52786219 1:201060865-201060865 10:91352966-91352966 9:129854199-129854199 18:43316538-43316538 4:17818885-17818885 5:31317529-31317529 19:48525507-48525507 15:99653800-99653800 17:38062217-38062217 3:47043538-47043538 12:69646914-69646914 3:122288210-122288210 2:205829991-205829991 17:4856580-4856580 22:43830996-43830996 10:124089036-124089036 13:32776603-32776603 1:62594593-62594593 10:128974621-128974621 5:180582604-180582604 20:23017082-23017082 9:123780005-123780005 6:147136244-147136244 5:169267784-169267784 3:4403837-4403837 10:75632760-75632760 19:53959343-53959343 7:17854532-17854532 12:119662572-119662572 8:6690276-6690276 12:102079418-102079418 20:48524827-48524827 1:157557701-157557701 10:101912064-101912064 10:124457906-124457906 2:30976058-30976058 21:47608580-47608580 15:90623052-90623052 8:61488824-61488824 1:13802325-13802325 13:46170609-46170609 10:91178000-91178000 19:56477710-56477710 8:142152442-142152442 7:150893629-150893629 9:136291063-136291063 19:39914748-39914748 19:19023853-19023853 11:133805585-133805585 19:6466482-6466482 3:89521693-89521693 3:191100561-191100561 19:54664752-54664752 4:121958091-121958091 7:138732497-138732497 8:72966002-72966002 11:10786142-10786142 9:73151970-73151970 12:49048738-49048738 14:75538217-75538217 17:76817090-76817090 19:12692001-12692001 20:744450-744450 21:43161877-43161877 14:21785790-21785790 2:48808594-48808594 3:44948479-44948479 1:229738170-229738170 3:109052732-109052732 3:132218623-132218623 1:180905448-180905448 2:218682771-218682771 2:70188676-70188676 7:18066569-18066569 8:3253768-3253768 16:68344696-68344696 4:154624656-154624656 3:44884647-44884647 8:104337096-104337096 17:3628362-3628362 18:56204932-56204932 1:248039713-248039713 8:125498547-125498547 8:2054171-2054171 20:20033380-20033380 7:99757612-99757612 16:79245820-79245820 3:69126495-69126495 16:30018500-30018500 17:3436080-3436080 12:52822258-52822258 8:130760850-130760850 19:44417575-44417575 18:6889973-6889973 9:139289825-139289825 11:73785326-73785326 2:158443889-158443889 7:6063283-6063283 3:40286017-40286017 20:44452697-44452697 1:24406647-24406647 22:19951271-19951271 3:14555216-14555216 22:51009953-51009953 8:17500223-17500223 6:51491884-51491884 10:8006798-8006798 17:8161149-8161149 5:179260213-179260213 5:137708434-137708434 15:84582124-84582124 3:108867128-108867128 4:78987157-78987157 5:176011889-176011889 8:121228679-121228679 16:74670458-74670458 1:201166383-201166383 3:196674749-196674749 17:17179481-17179481 11:104877927-104877927 11:75160575-75160575 9:130475011-130475011 5:78340286-78340286 13:78475313-78475313 9:79324741-79324741 7:24329133-24329133 11:55340379-55340379 11:66331458-66331458 22:30857448-30857448 22:43289473-43289473 22:45749983-45749983 17:72613589-72613589 12:125284748-125284748 19:11230881-11230881 20:44141331-44141331 20:23345844-23345844 20:371972-371972 20:25398790-25398790 20:14306953-14306953 11:65546857-65546857 20:25288632-25288632 11:120989335-120989335 2:49189921-49189921 18:6999628-6999628 13:47469940-47469940 2:234703136-234703136 7:91779971-91779971 15:42434254-42434254 14:65031534-65031534 20:61040951-61040951 1:225533684-225533684 1:156354367-156354367 1:182369233-182369233 1:158450314-158450314 2:234601669-234601669 3:14755572-14755572 11:65733393-65733393 5:34807928-34807928 5:35641582-35641582 2:21232195-21232195 15:89402596-89402596 1:211846876-211846876 9:21816758-21816758 1:181759657-181759657 19:17025292-17025292 10:29822159-29822159 10:116719543-116719543 8:95877787-95877787 12:3749874-3749874 14:23598952-23598952 14:31858209-31858209 14:21623648-21623648 15:78565478-78565478 16:89866043-89866043 17:1704296-1704296 19:57647015-57647015 12:109848567-109848567 13:32360547-32360547 20:43723627-43723627 1:242030151-242030151 22:44489896-44489896 2:71058835-71058835 8:124987478-124987478 1:110998854-110998854 1:205554095-205554095 1:229790048-229790048 1:157668390-157668390 1:160136350-160136350 20:39797465-39797465 2:241979550-241979550 17:3846802-3846802 17:15903602-15903602 2:113089267-113089267 3:134670524-134670524 17:3119767-3119767 17:3324255-3324255 5:73072354-73072354 5:118484804-118484804 12:56825311-56825311 6:42689755-42689755 8:143959250-143959250 4:98893476-98893476 10:97748040-97748040 10:16547058-16547058 10:69933969-69933969 12:124179417-124179417 12:56031273-56031273 12:4737042-4737042 13:31231806-31231806 17:11786951-11786951 3:138329862-138329862 1:156902259-156902259 22:44379838-44379838 3:158388780-158388780 1:158612236-158612236 16:4751045-4751045 14:59939727-59939727 8:27324822-27324822 4:154216710-154216710 17:37922259-37922259 8:25159885-25159885 5:121518309-121518309 1:170521376-170521376 9:7174673-7174673 9:112900199-112900199 12:93150102-93150102 3:44284584-44284584 2:26804247-26804247 15:75131959-75131959 2:191301368-191301368 21:27852724-27852724 17:38935812-38935812 3:193374964-193374964 3:171338237-171338237 17:48603503-48603503 17:45885756-45885756 18:43246156-43246156 1:117617705-117617705 | xargs samtools view -@ 4 -hb [input.bam] | samtools sort -@ 4 -o [output.bam]
Step 3
Upload the output bam
Step 4
Check the task status
Messages