As an essential part of the ongoing Farm animal Genotype-Tissue Expression (FarmGTEx) project, the ChickenGTEx aims to build a comprehensive public catalogue of genetic regulatory variants associated with multiple types of transcriptomic phenotypes in distinct biological contexts (e.g., tissues, cell types, development periods, gender, environmental exposures, and genetic backgrounds) in chickens. This resource will serve as a valuable reference for fundamental biology (e.g., genetic regulations related to development, immune response, and tissue/cell type specificity), bird or vertebrate evolution, comparative transcriptomes, applied genetics (e.g., chicken precision breeding, and food security), and human biomedicine.