| 实验编号 | CRX062571 |
| 物种名称 | Setaria italica |
| 标题 | PacBio Sequel sequencing of Xiaomi |
| 项目编号 | PRJCA001730 |
| 样本编号 | SAMC108142 |
| 测序平台 | PacBio Sequel |
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文库构建方法 |
建库策略 |
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The DNA libraries for PacBio SMRT sequencing were prepared following the PacBio standard protocols and sequenced on a Sequel platform (carried out by Biomarker Technologies Co; LTD, Beijing, China). Briefly, genomic DNA from a single Xiaomi plant was randomly sheared into an average size of 20 kb, using a gTube (Covaris Inc., Woburn, MA, USA) according to the manufacturer’s instructions. The sheared gDNA was end-repaired using polishing enzymes. After purification, a 20-kb insert SMRTbell library was constructed according to the PacBio standard protocol with the BluePippin size selection system (Sage Science, Beverly, USA) and sequences were generated on a PacBio Sequel (9 Cells) and PacBio RSII (1 cell) platform by Biomarker Technologies Co; LTD (Beijing, China). |
WGS |
GENOMIC |
size fractionation |
SINGLE
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| 发布日期 | 2020-06-24 |
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| 提交者 | Xingchun Wang (wxingchun@163.com) |
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| 所属单位 | Shanxi Agricultural University |
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| 提交日期 | 2019-09-17 |
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