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The DNA libraries for PacBio SMRT sequencing were prepared following the PacBio standard protocols and sequenced on a Sequel platform (carried out by Biomarker Technologies Co; LTD, Beijing, China). Briefly, genomic DNA from a single Xiaomi plant was randomly sheared into an average size of 20 kb, using a gTube (Covaris Inc., Woburn, MA, USA) according to the manufacturer’s instructions. The sheared gDNA was end-repaired using polishing enzymes. After purification, a 20-kb insert SMRTbell library was constructed according to the PacBio standard protocol with the BluePippin size selection system (Sage Science, Beverly, USA) and sequences were generated on a PacBio Sequel (9 Cells) and PacBio RSII (1 cell) platform by Biomarker Technologies Co; LTD (Beijing, China). |
WGS |
GENOMIC |
size fractionation |
SINGLE
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