Tibetan-Yi Corridor has long served as a vital agro-pastoral transitional zone, connecting the high-altitude Qinghai-Tibet Plateau with the lowland Central Plain, facilitating human migration for millennia. Despite its importance, the TYC population remains underrepresented in human genomic studies, limiting our understanding of their genetic origins and health. Here, we present a genomic resource from Austroasiatic and Sino-Tibetan individuals.