| Title |
GCTOF-MS Combined LC-QTRAP-MS/MS Reveals Metabolic Difference Between Osteoarthritis and Osteoporotic Osteoarthritis and the Intervention Effect of Erxian Decoction |
| Description |
The purpose of this study was to analyze the effect of OP on OA metabolites by using metabolomic methods and to explore the intervention mechanism of EXD on osteoporotic OA.Thirty-two SD rats were randomly divided into normal group, OA group, OP-OA group, and EXD group. EXD was administered by gavage. Histopathological evaluation of cartilage tissue was performed using Saffron fast green and HE staining. Western blot and qRT-PCR were used to detect the expression levels of chondrogenesis genes SOX9, COL2A1, and COMP in cartilage tissue. GC-TOFMS and LC-QTRAP-MS/MS metabolomics methods were used to analyze the changes of metabolites in serum samples of rats in each group. |
| Organism |
Rattus |
| Data Type |
Other Type of Metabolome Data |
| Data Accessibility |
Open-access |
| BioProject |
PRJCA010637 |
| Release Date |
2022-07-22 |
| Submitter |
Zhenyuan Ma (906459075@qq.com) |
| Organization |
Affiliated Hospital of Nanjing University of Chinese Medicine |
| Submission Date |
2022-07-20 |