qPCR data of a human embryonic limb cell atlas resolved in space and time project
| Title | qPCR data of a human embryonic limb cell atlas resolved in space and time project |
|---|---|
| Description | Our single-cell transcriptomic data of human embryonic limb have revealed that MSC, a transcriptional repressor, is expressed in PAX7+ muscle stem cells. To test whether MSC plays a role in repressing PAX7+ muscle stem cell differentiation, we knocked down MSC in human embryonic limb primary myoblasts. Our qPCR results showed profound up regulation of myocyte differentiation genes, suggesting that MSC is ia a key transcriptional repressor in maintaining the stemness of PAX7+ muscle stem cell. |
| Organism | Homo sapiens |
| Data Type | Other Type of Genomic Data |
| Data Accessibility | Controlled-access |
| BioProject | PRJCA012474 |
| Release Date | 2024-10-11 |
| Submitter | Hongbo Zhang (zhanghongbo@mail.sysu.edu.cn) |
| Organization | Sun Yat-sen University |
| Submission Date | 2022-10-17 |
The data cannot be downloaded as it has not yet been registered in the Human Genetic Resource Management Platform of MOST.
| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX002076-01 | qPCR detection of gene expression in primary myoblasts upon knockdown of MSC | 1 | Other Type of Genomic Data | 24.8 KB | xlsx | 0 | Unavailable |