| Title | Spatial deep isotope tracing highlights inter-tissue metabolic crosstalk |
|---|---|
| Description | Organs collaborate to maintain metabolic homeostasis in mammals. Spatial metabolomics makes strides in profiling the metabolic landscape, yet can not directly inspect the metabolic crosstalk between tissues. Here, we introduce an approach to comprehensively trace the metabolic fate of 13C-nutrients within the body and present a robust computational tool, MSIFluxer, to deep-probe metabolic activity in a spatial manner. Using ambient mass spectrometry imaging and isotope tracing, MSIFluxer empowers us to depict organ-specific metabolic characteristics by discerning the spatial distribution differences between newly synthesized labeled metabolites and native unlabeled ones. Importantly, we reveal that fatty acid metabolic crosstalk occurs between the liver and heart. Furthermore, we disclose metabolic coordination is lost system-wide in tumor-bearing mice and the host hexosamine biosynthesis pathway is considerably impacted by tumor burden. |
| Organism | Mus musculus |
| Data Type | Metabolome Data by Mass Spectrometry (MS) |
| Data Accessibility | Open-access |
| BioProject | PRJCA025174 |
| Release Date | 2025-06-30 |
| Submitter | Xinzhu Li (stxinzhu@outlook.com) |
| Organization | Institute of Materia Medica, Chinese Academy of Medical Sciences |
| Submission Date | 2024-04-11 |
HTTP download speed may be slow. It is highly recommended that you download the dataset using a dedicated FTP tool (such as FileZilla Client).
| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download |
|---|---|---|---|---|---|---|
| OMIX006225-01 | AMIDE-POS-Normal | 157 | Metabolome Data by Mass Spectrometry (MS) | 9.39 GB | zip | |
| OMIX006225-02 | C8-NEG-Normal | 156 | Metabolome Data by Mass Spectrometry (MS) | 23.26 GB | zip | |
| OMIX006225-03 | AMIDE-NEG-Normal | 162 | Metabolome Data by Mass Spectrometry (MS) | 8.28 GB | zip | |
| OMIX006225-04 | T3-NEG-Normal | 157 | Metabolome Data by Mass Spectrometry (MS) | 17.15 GB | zip | |
| OMIX006225-05 | C8-POS-Normal | 157 | Metabolome Data by Mass Spectrometry (MS) | 31.15 GB | zip | |
| OMIX006225-07 | T3-POS-Normal | 157 | Metabolome Data by Mass Spectrometry (MS) | 25.68 GB | zip |
| Paper Title | Journal Name | Publish Time | Accession | Citing Type |
|---|---|---|---|---|
| Spatial isotope deep tracing deciphers inter-tissue metabolic crosstalk | Nature Communications | 2025-08 | OMIX006225 | Deposit |