Estrogen oppositely regulates pulmonary hypertension via METTL3/PFKFB3 under normoxia and hypoxia
| Title | Estrogen oppositely regulates pulmonary hypertension via METTL3/PFKFB3 under normoxia and hypoxia |
|---|---|
| Description | PASMCs cultured under normoxia and hypoxia for 48 h were collected for the TMT-labeled quantitative proteomics analysis. The analysis was performed using aQ-Exactive mass spectrometer (Thermofisher, USA) by Shanghai Lu-Ming Biotech Co.,Ltd. (Shanghai, China). Briefly, PASMCs were lysed with 300 µL lysis buffer, followed by protein concentration measurement and quality control of the protein samples. Then the lyophilized samples were labeled with TMT. The mass spectrometry analysis using Proteome Discover 2.4 (Thermofisher, USA). Based on the criteria of Score Sequest HT more than 0 and unique peptide more than 0, the credible proteins were screened and the blank value was removed. The differentially expressed proteins were screened according to the criteria of fold change more than 1.5 or less than 0.67 and p-value less than 0.05, followed by GO enrichment analysis. |
| Organism | Rattus norvegicus |
| Data Type | Proteomic Data by Mass Spectrometry (MS) |
| Data Accessibility | Open-access |
| BioProject | PRJCA027246 |
| Release Date | 2024-10-14 |
| Submitter | Xiaosa Li (xiaosali@gzhmu.edu.cn) |
| Organization | Guangzhou Medical University |
| Submission Date | 2024-06-20 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX006712-01 | Raw data | 15 | Proteomic Data by Mass Spectrometry (MS) | 5.67 GB | zip | 0 |