A novel protein NSD1-916aa encoded by CircNSD1 contributes to AKI-to-CKD transition through inducing ferroptosis in renal tubular epithelial cells-3
| Title | A novel protein NSD1-916aa encoded by CircNSD1 contributes to AKI-to-CKD transition through inducing ferroptosis in renal tubular epithelial cells-3 |
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| Description | Proteins immunoprecipitated from SDS-PAGE gels were digested as previously described. The gel pieces were washed with 50% ACN for 30 min and then with 100% ACN for 30 min. Following reduction and alkylation, the proteins were digested with trypsin at 37 °C for 12 h. The supernatant containing the digested peptides was collected and processed for desalination using an HPLC buffer. The samples were analyzed by LC-MS/MS using an Easy-nLC1200 chromatograph (Thermo Scientific) and a Q Exactive plus mass spectrometer (Thermo Scientific). Furthermore, data analysis was performed using the predicted sequence of the NSD1-916aa protein and by searching against the UniProt-reviewed Homo sapiens database with Proteome Discoverer 2.4. |
| Organism | Homo sapiens |
| Data Type | Metabolome Data by Mass Spectrometry (MS) |
| Data Accessibility | Open-access |
| BioProject | PRJCA030078 |
| Release Date | 2024-09-13 |
| Submitter | Li Gao (18056052029@163.com) |
| Organization | The First Affiliated Hospital of Anhui Medical University |
| Submission Date | 2024-09-11 |
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| File ID | File Title | Number/Samples | File Type | File Size | File Suffix | Download Times | Download |
|---|---|---|---|---|---|---|---|
| OMIX007357-01 | DZLM2023120804 | 2 | Metabolome Data by Mass Spectrometry (MS) | 1.45 GB | zip | 0 |