| Title |
Histone lactylation drives liver cancer metastasis by facilitating NSF1 mediated ferroptosis resistance after microwave ablation |
| Description |
The separation was performed on an 1100 HPLC System (Agilent) using a Nano Chrom-C18 column. Mobile phases A (2% acetonitrile in HPLC water) and B (98% acetonitrile in HPLC water) were used for RP gradient. Tryptic peptides were separated at an fluent flow rate of 300 μL/min and monitored at 210 nm. Samples were collected for 8-53 minutes, and eluent was collected in centrifugal tube 1-15 every minute in turn.Samples were recycled in this order until the end of gradient. The separated peptides were lyophilized for mass spectrometry. All analyses were performed by a Fusion mass spectrometer equipped with a Easyspray source. Samples were loaded and separated by a capillary analytical column on an EASY-nLCTM 1200 system.The flow rate was 300 nL/min. Full MS scans were acquired in the mass range of 350- 1800 m/z with a mass resolution of 60000 and the AGC target value was set at 4e5. |
| Organism |
Homo sapiens |
| Data Type |
Proteomic Data by Mass Spectrometry (MS) |
| Data Accessibility |
Open-access |
| BioProject |
PRJCA031427 |
| Release Date |
2025-12-01 |
| Submitter |
Jiayan Huang (Sue66joo@163.com) |
| Organization |
West China Hospital of Sichuan University |
| Submission Date |
2024-10-21 |