OMIX

Protein profiling of hemocyte subpopulations in crayfish Cherax quadricarinatus

OMIX929

1Summary
Title Protein profiling of hemocyte subpopulations in crayfish Cherax quadricarinatus
Description Semigranular cells (SGCs) and granular cells (GCs) are two dominant groups of circulating hemocytes in crayfish Cherax quadricarinatus. In this study, the protein profiles of different hemocyte subpopulations and their progenitors were analyzed by two work-flows. Firstly, the cytoplasmic fractions and membrane fractions were prepared from SGCs and GCs. The protein fractions were analyzed by one-dimensional gel electrophoresis, and the cell type-specific proteins were identified by LC-MS/MS. In addition, the cytoplasmic fractions and membrane fractions were prepared from hematopoietic tissue cells, SGCs, and GCs. These protein fractions were analyzed by in-solution digestion followed by LC-MS/MS. The cell type-specific proteins/differentially expressed proteins were also screened out by this method.
Organism Cherax quadricarinatus
Data Type -
Data Accessibility Open-access
BioProject PRJCA008179
Release Date 2022-02-09
Submitter Fang Li (lifang@tio.org.cn)
Organization Third Institute of Oceanography, Ministry of Natural Resources
Submission Date 2022-02-09
2Files & Download

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File ID File Title Number/Samples File Type File Size File Suffix Download Times Download
OMIX929-10-01 LC-MS/MS results 1 Proteomic 7.8 MB rar 0

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