OBJECTIVES: Hygroryza aristata, an aquatic plant native to Southeast Asia, shows a high degree of adaptability to aquatic environments. H. aristata, which belongs to the Oryzeae tribe and is closely related to rice (Oryza sativa), holds potential for crop improvement, particularly in flood tolerance. This study aimed to sequence and assemble the genome of H. aristata.
DATA DESCRIPTION: We assembled the genome of H. aristata using 31.91 Gb of Pacific Biosciences (PacBio) High-fidelity (HiFi) data and 22.36 Gb of ultra long Oxford Nanopore Technology (ONT) data, resulting in two gap-free haplotype genomes, hap1 (349.74 Mb) and hap2 (347.98 Mb), each with 12 chromosomes and 23 telomeres. The continuity of chromosomes was supported by High-throughput chromosome conformation capture (Hi-C) data. The assemblies demonstrated high completeness, with > 99.8% of coverage rates, 98.4% of Benchmarking Universal Single-Copy Orthologs (BUSCO) scores, and > 11.0 of Long Terminal Repeat Assembly Index (LAI) scores per haplotype. RNA sequencing (RNA-seq) data (176.06 Gb) of six tissues was generated for genome annotation, identifying 39,139 and 38,746 protein-coding genes in hap1 and hap2, respectively.
Nucleic Acids Res. 2018 Nov 30;46(21):e126
[PMID:
30107434]
Nat Commun. 2024 Apr 17;15(1):3305
[PMID:
38632270]
BMC Genom Data. 2025 Mar 28;26(1):23
[PMID:
40148752]
Cell Syst. 2016 Jul;3(1):95-8
[PMID:
27467249]
Bioinformatics. 2015 Oct 1;31(19):3210-2
[PMID:
26059717]
Nat Methods. 2021 Feb;18(2):170-175
[PMID:
33526886]
Curr Opin Plant Biol. 2003 Apr;6(2):139-46
[PMID:
12667870]
Mol Plant. 2023 Aug 7;16(8):1232-1236
[PMID:
37553831]
Bioinformatics. 2010 Mar 1;26(5):589-95
[PMID:
20080505]
Mitochondrial DNA B Resour. 2021 Jun 22;6(7):1949-1950
[PMID:
34212082]
Bioinformatics. 2021 Dec 7;37(23):4572-4574
[PMID:
34623391]
BMC Plant Biol. 2024 Jul 8;24(1):644
[PMID:
38973002]
Genome Biol. 2019 Dec 16;20(1):275
[PMID:
31843001]
Genome Res. 2024 Jun 25;34(5):769-777
[PMID:
38866550]