AHG11 - Plant Editosome Database

Summary

Editing Factor:	AHG11
Synonym:	ABA HYPERSENSITIVE GERMINATION 11
Description:	The geminating ahg11 seeds have higher levels of reactive-oxygen-species-responsive genes; Presumably, partial impairment of mitochondrial function caused by an amino acid conversion in one of the complex I components induces redox imbalance which, in turn, confers an abnormal response to the plant hormone; Encodes an E+ PLS family protein that plays a role in mRNA processing in mitochondria, particularly in RNA editing; It is required for germination or early seedling growth stages; Mutant of AHG11 show a weak ABA hypersensitive phenotype
Protein Family:	PPR
Subclass:	E+
Physical Interaction:	NA
Construct Structure:	PLS-E+, PLS-E-E+
Gene ID & Species:	AT2G44880 (Arabidopsis thaliana)
Edited Gene(s):	nad4
Editing Type(s):	C-to-U (7)
Publication(s):	[1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170] [2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003] [3] Isolation of Arabidopsis ahg11, a weak ABA hypersensitive mutant defective in nad4 RNA editing., Journal of experimental botany, 2012. [PMID=22821940]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT2G44880

Mitochondrion

nad4

376

CDS

EnsemblGenomes (release 39)
NA

BK010421
NA

C-to-U

NA=>NA

NA=>NA
R=>C

NA
Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	99.58%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	46.00%	Medium	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	31.00%	Low	Decreased	28761003
Columbia (Col-0)	WT	Control	Control	No mutant	A normal ABA response at germination. Normal sensitivities to glucose and methyl jasmonic acid at germination.	Seedling	NA	Sequencing PCR products	100.00%	Complete	None	22821940
NA	ahg11	ABA hypersensitive germination	NA	Recessive	A weak ABA hypersensitive phenotype at the seed or young seedling stages	Seedling	NA	Sequencing PCR products	8.77%	Poor	Decreased	22821940
NA	ahg11+AHG11	Complementation	The genomic AHG11 DNA fragment was obtained by PCR using high-fidelity DNA polymerase (KOD-Plus; Toyobo, Osaka, Japan) with primers, F-BamHpro2 and R-terXbaI, and subcloned into pBluescript SK(-). Aft	Recessive	A normal ABA response at germination. Normal sensitivities to glucose and methyl jasmonic acid at germination.	Seedling	NA	Sequencing PCR products	100.00%	Complete	Restored	22821940