AHG11 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: AHG11
Synonym: ABA HYPERSENSITIVE GERMINATION 11
Description: The geminating ahg11 seeds have higher levels of reactive-oxygen-species-responsive genes; Presumably, partial impairment of mitochondrial function caused by an amino acid conversion in one of the complex I components induces redox imbalance which, in turn, confers an abnormal response to the plant hormone; Encodes an E+ PLS family protein that plays a role in mRNA processing in mitochondria, particularly in RNA editing; It is required for germination or early seedling growth stages; Mutant of AHG11 show a weak ABA hypersensitive phenotype
Protein Family: PPR
Subclass: E+
Construct Structure: PLS-E+, PLS-E-E+
Gene ID & Species: AT2G44880 (Arabidopsis thaliana)
Edited Gene(s): nad4
Editing Type(s): C-to-U (7)
Publication(s): [1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003]
[3] Isolation of Arabidopsis ahg11, a weak ABA hypersensitive mutant defective in nad4 RNA editing., Journal of experimental botany, 2012. [PMID=22821940]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT2G44880 Mitochondrion nad4 376 CDS C-to-U NA=>NA NA=>NA
R=>C
NA
Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNANANARNA-seq99.58%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract46.00%MediumDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract31.00%LowDecreased28761003
Columbia (Col-0)WTControlControlNo mutantA normal ABA response at germination. Normal sensitivities to glucose and methyl jasmonic acid at germination.SeedlingNASequencing PCR products100.00%CompleteNone22821940
NAahg11ABA hypersensitive germinationNARecessiveA weak ABA hypersensitive phenotype at the seed or young seedling stagesSeedlingNASequencing PCR products8.77%PoorDecreased22821940
NAahg11+AHG11ComplementationThe genomic AHG11 DNA fragment was obtained by PCR using high-fidelity DNA polymerase (KOD-Plus; Toyobo, Osaka, Japan) with primers, F-BamHpro2 and R-terXbaI, and subcloned into pBluescript SK(-). AftRecessiveA normal ABA response at germination. Normal sensitivities to glucose and methyl jasmonic acid at germination.SeedlingNASequencing PCR products100.00%CompleteRestored22821940
Last update: Jul 2021 (version 1.0)