CRR28 - Plant Editosome Database

Summary

Editing Factor:	CRR28
Synonym:	CHLORORESPIRATORY REDUCTION 28
Description:	The DYW proteins CHLORORESPIRATORY REDUCTION22 (CRR22) and CRR28 are required for editing of multiple plastid transcripts but that their DYW motifs are dispensable for editing activity in vivo; Replacement of the DYW motifs of CRR22 and CRR28 by that of CRR2, which has been shown to be capable of endonucleolytic cleavage, blocks the editing activity of both proteins; In return, the DYW motifs of neither CRR22 nor CRR28 can functionally replace that of CRR2; Different DYW family members have acquired distinct functions in the divergent processes of RNA maturation, including RNA cleavage and RNA editing
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	DYW
Physical Interaction:	NA
Construct Structure:	PLS-E-DYW
Gene ID & Species:	AT1G59720 (Arabidopsis thaliana)
Edited Gene(s):	ndhB ndhD
Editing Type(s):	C-to-U (24)
Publication(s):	[1] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [doi:10.1073/pnas.1705815114] [2] Pentatricopeptide repeat proteins with the DYW motif have distinct molecular functions in RNA editing and RNA cleavage in Arabidopsis chloroplasts, The Plant Cell, 2009. [doi:10.1105/tpc.108.064667]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT1G59720

Chloroplast

ndhB

467

CDS

Arabidopsis Genome Initiative
NA

AP000423
NA

C-to-U

CCA=>CUA
NA=>NA

P=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	Col-0	Control	Control	No mutant	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	100.00%	Complete	None	19182104
Ler	crr28-1	Ds Insertion	NA	Recessive	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
Ler	crr28-1+CRR28DYW2	Complementation	crr28-1 is transformed with CRR28, in which the DYW motif is replaced by that of CRR2	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19182104
Ler	crr28-1+CRR28DYW22	Complementation	crr28-1 is transformed with CRR28, in which the DYW motif is replaced by that of CRR22	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	100.00%	Complete	Restored	19182104
Ler	crr28-1+CRR28△DYW	Complementation	crr28-1 is transformed with CRR28 lacking the DYW motif	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	100.00%	Complete	Restored	19182104
Ler	crr28-1+CRR28△E/DYW	Complementation	crr28-1 is transformed with CRR28 lacking the E and DYW motifs	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19182104
SALK_115133	crr28-2	T-DNA Insertion	NA	NA	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
SALK_012455	crr28-3	T-DNA Insertion	NA	NA	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
Ler	Ler	Control	Control	No mutant	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	100.00%	Complete	None	19182104
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	82.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	99.00%	High	Increased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	99.00%	High	Increased	28761003