CRR28 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT1G59720

Chloroplast
Plastid

ndhB

467

CDS

C-to-U

CCA=>CUA
NA=>NA

P=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	Col-0	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	100.00%	Complete	None	19182104
Ler	crr28-1	Ds Insertion	NA	Recessive mutation	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
Ler	crr28-1+CRR28DYW2	Complementation	crr28-1 is transformed with CRR28, in which the DYW motif is replaced by that of CRR2	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19182104
Ler	crr28-1+CRR28DYW22	Complementation	crr28-1 is transformed with CRR28, in which the DYW motif is replaced by that of CRR22	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	100.00%	Complete	Restored	19182104
Ler	crr28-1+CRR28△DYW	Complementation	crr28-1 is transformed with CRR28 lacking the DYW motif	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	100.00%	Complete	Restored	19182104
Ler	crr28-1+CRR28△E/DYW	Complementation	crr28-1 is transformed with CRR28 lacking the E and DYW motifs	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19182104
SALK_115133	crr28-2	T-DNA Insertion	NA	NA	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
SALK_012455	crr28-3	T-DNA Insertion	NA	NA	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
Ler	Ler	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	100.00%	Complete	None	19182104
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	87.52%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	82.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	99.00%	High	Increased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	99.00%	High	Increased	28761003

Arabidopsis thaliana

AT1G59720

Chloroplast
Plastid

ndhD

878

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	Col-0	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	100.00%	Complete	None	19182104
Ler	crr28-1	Ds Insertion	NA	Recessive mutation	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
Ler	crr28-1+CRR28DYW2	Complementation	crr28-1 is transformed with CRR28, in which the DYW motif is replaced by that of CRR2	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19182104
Ler	crr28-1+CRR28DYW22	Complementation	crr28-1 is transformed with CRR28, in which the DYW motif is replaced by that of CRR22	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	100.00%	Complete	Restored	19182104
Ler	crr28-1+CRR28△DYW	Complementation	crr28-1 is transformed with CRR28 lacking the DYW motif	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	100.00%	Complete	Restored	19182104
Ler	crr28-1+CRR28△E/DYW	Complementation	crr28-1 is transformed with CRR28 lacking the E and DYW motifs	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	19182104
SALK_115133	crr28-2	T-DNA Insertion	NA	NA	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
SALK_012455	crr28-3	T-DNA Insertion	NA	NA	Impaired NDH activity	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19182104
Ler	Ler	Control	Control	Control	Normal	Leaf	4 weeks	Poisoned Primer Extension (PPE) Assay	100.00%	Complete	None	19182104
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	83.91%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	88.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Increased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Increased	28761003

Editing Factor:	CRR28
Synonym:	CHLORORESPIRATORY REDUCTION28
Description:	Encoding a PPR-DYW protein with endonuclease activity required for editing of multiple plastid transcripts
Protein Family:	PPR
Subclass:	DYW
Construct Structure:	PLS-E-DYW
Gene ID & Species:	AT1G59720 (Arabidopsis thaliana)
Edited Gene(s):	ndhB ndhD
Editing Type(s):	C-to-U (26)
Publication(s):	[1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170] [2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003] [3] Pentatricopeptide repeat proteins with the DYW motif have distinct molecular functions in RNA editing and RNA cleavage in Arabidopsis chloroplasts., The Plant cell, 2009. [PMID=19182104]

Plant Editosome Database
a curated database of RNA editosome in plants

Summary

Editing Details