Plant Editosome Database
a curated database of RNA editosome in plants

CWM1 - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: CWM1
Synonym: CELL WALL MAINTAINER 1
Description: Encoding a PPR protein involved in mitochondrial functioning; Mutants suppress cell wall defects caused by C17 chemical inhibitor; Mutants are defective in cytochrome c maturation and activation of mitochondrial retrograde signalling
Protein Family: PPR
Subclass: E
Construct Structure: PLS-E1-E2
Gene ID & Species: AT1G17630 (Arabidopsis thaliana)
NA (Macadamia integrifolia)
Edited Gene(s): ccmB    nad5    ccmC
Editing Type(s): C-to-U (65)
Publication(s): [1] One C-to-U RNA Editing Site and Two Independently Evolved Editing Factors: Testing Reciprocal Complementation with DYW-Type PPR Proteins from the Moss () and the Flowering Plants and Arabidopsis., The Plant cell, 2020. [PMID=32616665]
[2] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[3] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003]
[4] Mitochondrial Defects Confer Tolerance against Cellulose Deficiency., The Plant cell, 2016. [PMID=27543091]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT1G17630 Mitochondrion ccmB 428 CDS C-to-U UCG=>UUG
NA=>NA		 S=>L
NA=>NA		 Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalRoot5-day-oldRT-PCR and direct sequencing89.29%HighNone27543091
SALK_017325Ccwm1-1T-DNA insertionA T-DNA insertion is approximately at +448bp in the exon in AT1G17630NAHigher respiration than the wild type;C17 toleranceRoot5-day-oldRT-PCR and direct sequencing29.63%LowDecreased27543091
SALK_124160cwm1-2T-DNA insertionA T-DNA insertion is approximately at +624bp in the exon in AT1G17630NAHigher respiration than the wild type;C17 toleranceRoot5-day-oldRT-PCR and direct sequencing37.50%LowDecreased27543091
Col-0WTControlControlControlNANANARNA-seq95.55%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract68.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract30.00%LowDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract15.00%PoorDecreased28761003
Col-0WTControlControlControlNormalLeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat100.00%CompleteNone32616665
SALK_017325Ccwm1-1A T-DNA InsertionNAHomozygousA mild root growth phenotype and reduced seed germination rates on soil.Leaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat0.00%UneditedAbsent32616665
SALK_124160cwm1-2A T-DNA InsertionNAHomozygousA mild root growth phenotype and reduced seed germination rates on soil.Leaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat14.00%PoorDecreased32616665
Col-0A. thaliana CWM1Transformation and ComplementationThe Arabidopsis cwm1 mutants were transformed with the Arabidopsis CWM1 gene.HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat100.00%CompleteRestored32616665
Col-0P. patens PPR79Transformation and ComplementationExpressing the original PPR79 coding sequence in the cwm1 background.HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat73.00%HighDecreased32616665
Col-0P. patens PPR79△YWTransformation and ComplementationExpressing the original PPR79 coding sequence lacking the C-terminal DYW domain in the cwm1 background.HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat85.00%HighDecreased32616665
Col-0CWM1-DYWPPR79Transformation and ComplementationAdding the DYW domain from PPR79 after the PG-Box of CWM1 (CWM1-DYWPPR79), expressed in the cwm1 background.HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat100.00%CompleteRestored32616665
Col-0CWM1PLS-E1E2DYWPPR79Transformation and ComplementationReplacing the complete set of C-terminal domains from CWM1 with those from PPR79 (CWM1PLS-E1E2DYWPPR79), expressed in the cwm1 background.HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat100.00%CompleteRestored32616665
NAAt cwm1 ko complementation Line 1Transformation and ComplementationCWM1 targets transgenic lines in the Arabidopsis cwm1 mutant expressing Macadamia CWM1,HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat0.00%UneditedAbsent32616665
NAAt cwm1 ko complementation Line 2Transformation and ComplementationCWM1 targets transgenic lines in the Arabidopsis cwm1 mutant expressing Macadamia CWM1,HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat27.00%LowDecreased32616665
Arabidopsis thaliana AT1G17630 Mitochondrion ccmC 463 CDS C-to-U CGU=>UGU
NA=>NA		 R=>C
NA=>NA		 Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalRoot5-day-oldRT-PCR and direct sequencing100.00%CompleteNone27543091
SALK_017325Ccwm1-1T-DNA insertionA T-DNA insertion is approximately at +448bp in the exon in AT1G17630NAHigher respiration than the wild type;C17 toleranceRoot5-day-oldRT-PCR and direct sequencing10.71%PoorDecreased27543091
SALK_124160cwm1-2T-DNA insertionA T-DNA insertion is approximately at +624bp in the exon in AT1G17630NAHigher respiration than the wild type;C17 toleranceRoot5-day-oldRT-PCR and direct sequencing17.86%PoorDecreased27543091
Col-0WTControlControlControlNANANARNA-seq94.11%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract94.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract0.00%UneditedAbsent28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract5.00%PoorDecreased28761003
Col-0WTControlControlControlNormalLeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat100.00%CompleteNone32616665
SALK_017325Ccwm1-1A T-DNA InsertionNAHomozygousA mild root growth phenotype and reduced seed germination rates on soil.Leaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat0.00%UneditedAbsent32616665
SALK_124160cwm1-2A T-DNA InsertionNAHomozygousA mild root growth phenotype and reduced seed germination rates on soil.Leaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat0.00%UneditedAbsent32616665
Col-0A. thaliana CWM1Transformation and ComplementationThe Arabidopsis cwm1 mutants were transformed with the Arabidopsis CWM1 gene.HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat93.00%HighRestored32616665
Col-0P. patens PPR79Transformation and ComplementationExpressing the original PPR79 coding sequence in the cwm1 background.HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat34.00%LowDecreased32616665
Col-0P. patens PPR79△YWTransformation and ComplementationExpressing the original PPR79 coding sequence lacking the C-terminal DYW domain in the cwm1 background.HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat62.00%HighDecreased32616665
Col-0CWM1-DYWPPR79Transformation and ComplementationAdding the DYW domain from PPR79 after the PG-Box of CWM1 (CWM1-DYWPPR79), expressed in the cwm1 background.HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat100.00%CompleteRestored32616665
Col-0CWM1PLS-E1E2DYWPPR79Transformation and ComplementationReplacing the complete set of C-terminal domains from CWM1 with those from PPR79 (CWM1PLS-E1E2DYWPPR79), expressed in the cwm1 background.HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat100.00%CompleteRestored32616665
NAAt cwm1 ko complementation Line 1Transformation and ComplementationCWM1 targets transgenic lines in the Arabidopsis cwm1 mutant expressing Macadamia CWM1,HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat100.00%CompleteRestored32616665
NAAt cwm1 ko complementation Line 2Transformation and ComplementationCWM1 targets transgenic lines in the Arabidopsis cwm1 mutant expressing Macadamia CWM1,HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat100.00%CompleteRestored32616665
Arabidopsis thaliana AT1G17630 Mitochondrion nad5 598 CDS C-to-U CGU=>UGU
NA=>NA		 R=>C
NA=>NA		 Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalRoot5-day-oldRT-PCR and direct sequencing100.00%CompleteNone27543091
SALK_017325Ccwm1-1T-DNA insertionA T-DNA insertion is approximately at +448bp in the exon in AT1G17630NAHigher respiration than the wild type;C17 toleranceRoot5-day-oldRT-PCR and direct sequencing7.89%PoorDecreased27543091
SALK_124160cwm1-2T-DNA insertionA T-DNA insertion is approximately at +624bp in the exon in AT1G17630NAHigher respiration than the wild type;C17 toleranceRoot5-day-oldRT-PCR and direct sequencing5.41%PoorDecreased27543091
Col-0WTControlControlControlNANANARNA-seq99.09%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract99.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract22.00%LowDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract9.00%PoorDecreased28761003
Col-0WTControlControlControlNormalLeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat100.00%CompleteNone32616665
SALK_017325Ccwm1-1A T-DNA InsertionNAHomozygousA mild root growth phenotype and reduced seed germination rates on soil.Leaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat0.00%UneditedAbsent32616665
SALK_124160cwm1-2A T-DNA InsertionNAHomozygousA mild root growth phenotype and reduced seed germination rates on soil.Leaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat0.00%UneditedAbsent32616665
Col-0A. thaliana CWM1Transformation and ComplementationThe Arabidopsis cwm1 mutants were transformed with the Arabidopsis CWM1 gene.HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat100.00%CompleteRestored32616665
Col-0P. patens PPR79Transformation and ComplementationExpressing the original PPR79 coding sequence in the cwm1 background.HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat51.00%MediumDecreased32616665
Col-0P. patens PPR79△YWTransformation and ComplementationExpressing the original PPR79 coding sequence lacking the C-terminal DYW domain in the cwm1 background.HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat70.00%HighDecreased32616665
Col-0CWM1-DYWPPR79Transformation and ComplementationAdding the DYW domain from PPR79 after the PG-Box of CWM1 (CWM1-DYWPPR79), expressed in the cwm1 background.HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat100.00%CompleteRestored32616665
Col-0CWM1PLS-E1E2DYWPPR79Transformation and ComplementationReplacing the complete set of C-terminal domains from CWM1 with those from PPR79 (CWM1PLS-E1E2DYWPPR79), expressed in the cwm1 background.HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat100.00%CompleteRestored32616665
NAAt cwm1 ko complementation Line 1Transformation and ComplementationCWM1 targets transgenic lines in the Arabidopsis cwm1 mutant expressing Macadamia CWM1,HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat100.00%CompleteRestored32616665
NAAt cwm1 ko complementation Line 2Transformation and ComplementationCWM1 targets transgenic lines in the Arabidopsis cwm1 mutant expressing Macadamia CWM1,HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat71.00%HighDecreased32616665
Arabidopsis thaliana AT1G17630 Mitochondrion nad5 609 CDS C-to-U GUC=>GUU V=>V Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalLeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat86.00%HighNone32616665
SALK_017325Ccwm1-1A T-DNA InsertionNAHomozygousA mild root growth phenotype and reduced seed germination rates on soil.Leaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat27.00%LowDecreased32616665
SALK_124160cwm1-2A T-DNA InsertionNAHomozygousA mild root growth phenotype and reduced seed germination rates on soil.Leaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat25.00%LowDecreased32616665
Col-0A. thaliana CWM1Transformation and ComplementationThe Arabidopsis cwm1 mutants were transformed with the Arabidopsis CWM1 gene.HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat73.00%HighDecreased32616665
Col-0P. patens PPR79Transformation and ComplementationExpressing the original PPR79 coding sequence in the cwm1 background.HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat44.00%MediumDecreased32616665
Col-0P. patens PPR79△YWTransformation and ComplementationExpressing the original PPR79 coding sequence lacking the C-terminal DYW domain in the cwm1 background.HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat29.00%LowDecreased32616665
Col-0CWM1-DYWPPR79Transformation and ComplementationAdding the DYW domain from PPR79 after the PG-Box of CWM1 (CWM1-DYWPPR79), expressed in the cwm1 background.HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat73.00%HighDecreased32616665
Col-0CWM1PLS-E1E2DYWPPR79Transformation and ComplementationReplacing the complete set of C-terminal domains from CWM1 with those from PPR79 (CWM1PLS-E1E2DYWPPR79), expressed in the cwm1 background.HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat57.00%MediumDecreased32616665
NAAt cwm1 ko complementation Line 1Transformation and ComplementationCWM1 targets transgenic lines in the Arabidopsis cwm1 mutant expressing Macadamia CWM1,HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat100.00%CompleteRestored32616665
NAAt cwm1 ko complementation Line 2Transformation and ComplementationCWM1 targets transgenic lines in the Arabidopsis cwm1 mutant expressing Macadamia CWM1,HomozygousNALeaf2- to 3-week oldcDNA sequencing by Macrogen Europe, and peak height calculat64.00%HighDecreased32616665
Macadamia integrifolia NA Mitochondrion ccmB 428 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAM. integrifolia CWM1ControlControlControlNANANAcDNA sequencing by Macrogen Europe, and peak height calculat41.00%MediumNone32616665
Macadamia integrifolia NA Mitochondrion ccmC 463 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAM. integrifolia CWM1ControlControlControlNANANAcDNA sequencing by Macrogen Europe, and peak height calculat100.00%CompleteNone32616665
Macadamia integrifolia NA Mitochondrion nad5 598 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAM. integrifolia CWM1ControlControlControlNANANAcDNA sequencing by Macrogen Europe, and peak height calculat100.00%CompleteNone32616665
Macadamia integrifolia NA Mitochondrion nad5 609 CDS C-to-U GUC=>GUU V=>V Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAM. integrifolia CWM1ControlControlControlNANANAcDNA sequencing by Macrogen Europe, and peak height calculat0.00%UneditedNone32616665
Last update: Jul 2021 (version 1.0)