CWM1 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: CWM1
Synonym: CELL WALL MAINTAINER 1
Description: Encodes a PPR protein involved in mitochondrial functioning. Mutants suppress cell wall defects caused by C17 chemical inhibitor. Mutants are defective in cytochrome c maturation and activation of mitochondrial retrograde signalling.
Protein Family: PPR
Subclass: E
Construct Structure: PLS-E1-E2
Gene ID & Species: AT1G17630 (Arabidopsis thaliana)
NA (Macadamia integrifolia)
Edited Gene(s): ccmB    nad5    ccmC
Editing Type(s): C-to-U (65)
Publication(s): [1] One C-to-U RNA Editing Site and Two Independently Evolved Editing Factors: Testing Reciprocal Complementation with DYW-Type PPR Proteins from the Moss Physcomitrium (Physcomitrella) patens and the Flowering Plants Macadamia integrifolia and Arabidopsis, Plant Cell, 2020. [PMID=32616665]
[2] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[3] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003]
[4] Mitochondrial Defects Confer Tolerance against Cellulose Deficiency, Plant Cell, 2016. [PMID=27543091]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT1G17630 Mitochondrion ccmB 428 CDS C-to-U UCG=>UUG
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatment No treatmentNo treatmentNormalRoot5-day-oldRT-PCR and direct sequencing89.29%HighNone27543091
SALK_017325Ccwm1-1T-DNA insertionA T-DNA insertion is approximately at +448bp in the exon in AT1G17630NAHigher respiration than the wild type;C17 toleranceRoot5-day-oldRT-PCR and direct sequencing29.63%LowDecreased27543091
SALK_124160cwm1-2T-DNA insertionA T-DNA insertion is approximately at +624bp in the exon in AT1G17630NAHigher respiration than the wild type;C17 toleranceRoot5-day-oldRT-PCR and direct sequencing37.50%LowDecreased27543091
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq95.55%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract68.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract30.00%LowDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract15.00%PoorDecreased28761003
Col-0WTNo treatmentNo treatmentNo treatmentNormalLeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with100.00%CompleteNone32616665
SALK_017325Ccwm1-1A T-DNA InsertionNAHomozygousA mild root growth phenotype and reduced seed germination rates on soil.Leaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with0.00%UneditedAbsent32616665
SALK_124160cwm1-2A T-DNA InsertionNAHomozygousA mild root growth phenotype and reduced seed germination rates on soil.Leaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with14.00%PoorDecreased32616665
Col-0A. thaliana CWM1Transformation and ComplementationThe Arabidopsis cwm1 mutants were transformed with the Arabidopsis CWM1 gene.HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with100.00%CompleteRestored32616665
Col-0P. patens PPR79Transformation and ComplementationExpressing the original PPR79 coding sequence in the cwm1 background.HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with73.00%HighDecreased32616665
Col-0P. patens PPR79△DYWTransformation and ComplementationExpressing the original PPR79 coding sequence lacking the C-terminal DYW domain in the cwm1 background.HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with85.00%HighDecreased32616665
Col-0CWM1-DYWPPR79Transformation and ComplementationAdding the DYW domain from PPR79 after the PG-Box of CWM1 (CWM1-DYWPPR79), expressed in the cwm1 background.HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with100.00%CompleteRestored32616665
Col-0CWM1PLS-E1E2DYWPPR79Transformation and ComplementationReplacing the complete set of C-terminal domains from CWM1 with those from PPR79 (CWM1PLS-E1E2DYWPPR79), expressed in the cwm1 background.HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with100.00%CompleteRestored32616665
NAAt cwm1 ko complementation Line 1Transformation and ComplementationCWM1 targets transgenic lines in the Arabidopsis cwm1 mutant expressing Macadamia CWM1,HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with0.00%UneditedAbsent32616665
NAAt cwm1 ko complementation Line 2Transformation and ComplementationCWM1 targets transgenic lines in the Arabidopsis cwm1 mutant expressing Macadamia CWM1,HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with27.00%LowDecreased32616665
Arabidopsis thaliana AT1G17630 Mitochondrion ccmC 463 CDS C-to-U CGU=>UGU
NA=>NA
R=>C
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatment No treatmentNo treatmentNormalRoot5-day-oldRT-PCR and direct sequencing100.00%CompleteNone27543091
SALK_017325Ccwm1-1T-DNA insertionA T-DNA insertion is approximately at +448bp in the exon in AT1G17630NAHigher respiration than the wild type;C17 toleranceRoot5-day-oldRT-PCR and direct sequencing10.71%PoorDecreased27543091
SALK_124160cwm1-2T-DNA insertionA T-DNA insertion is approximately at +624bp in the exon in AT1G17630NAHigher respiration than the wild type;C17 toleranceRoot5-day-oldRT-PCR and direct sequencing17.86%PoorDecreased27543091
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq94.11%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract94.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract0.00%UneditedAbsent28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract5.00%PoorDecreased28761003
Col-0WTNo treatmentNo treatmentNo treatmentNormalLeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with100.00%CompleteNone32616665
SALK_017325Ccwm1-1A T-DNA InsertionNAHomozygousA mild root growth phenotype and reduced seed germination rates on soil.Leaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with0.00%UneditedAbsent32616665
SALK_124160cwm1-2A T-DNA InsertionNAHomozygousA mild root growth phenotype and reduced seed germination rates on soil.Leaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with0.00%UneditedAbsent32616665
Col-0A. thaliana CWM1Transformation and ComplementationThe Arabidopsis cwm1 mutants were transformed with the Arabidopsis CWM1 gene.HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with93.00%HighRestored32616665
Col-0P. patens PPR79Transformation and ComplementationExpressing the original PPR79 coding sequence in the cwm1 background.HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with34.00%LowDecreased32616665
Col-0P. patens PPR79△DYWTransformation and ComplementationExpressing the original PPR79 coding sequence lacking the C-terminal DYW domain in the cwm1 background.HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with62.00%HighDecreased32616665
Col-0CWM1-DYWPPR79Transformation and ComplementationAdding the DYW domain from PPR79 after the PG-Box of CWM1 (CWM1-DYWPPR79), expressed in the cwm1 background.HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with100.00%CompleteRestored32616665
Col-0CWM1PLS-E1E2DYWPPR79Transformation and ComplementationReplacing the complete set of C-terminal domains from CWM1 with those from PPR79 (CWM1PLS-E1E2DYWPPR79), expressed in the cwm1 background.HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with100.00%CompleteRestored32616665
NAAt cwm1 ko complementation Line 1Transformation and ComplementationCWM1 targets transgenic lines in the Arabidopsis cwm1 mutant expressing Macadamia CWM1,HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with100.00%CompleteRestored32616665
NAAt cwm1 ko complementation Line 2Transformation and ComplementationCWM1 targets transgenic lines in the Arabidopsis cwm1 mutant expressing Macadamia CWM1,HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with100.00%CompleteRestored32616665
Arabidopsis thaliana AT1G17630 Mitochondrion nad5 598 CDS C-to-U CGU=>UGU
NA=>NA
R=>C
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatment No treatmentNo treatmentNormalRoot5-day-oldRT-PCR and direct sequencing100.00%CompleteNone27543091
SALK_017325Ccwm1-1T-DNA insertionA T-DNA insertion is approximately at +448bp in the exon in AT1G17630NAHigher respiration than the wild type;C17 toleranceRoot5-day-oldRT-PCR and direct sequencing7.89%PoorDecreased27543091
SALK_124160cwm1-2T-DNA insertionA T-DNA insertion is approximately at +624bp in the exon in AT1G17630NAHigher respiration than the wild type;C17 toleranceRoot5-day-oldRT-PCR and direct sequencing5.41%PoorDecreased27543091
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq99.09%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract99.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract22.00%LowDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract9.00%PoorDecreased28761003
Col-0WTNo treatmentNo treatmentNo treatmentNormalLeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with100.00%CompleteNone32616665
SALK_017325Ccwm1-1A T-DNA InsertionNAHomozygousA mild root growth phenotype and reduced seed germination rates on soil.Leaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with0.00%UneditedAbsent32616665
SALK_124160cwm1-2A T-DNA InsertionNAHomozygousA mild root growth phenotype and reduced seed germination rates on soil.Leaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with0.00%UneditedAbsent32616665
Col-0A. thaliana CWM1Transformation and ComplementationThe Arabidopsis cwm1 mutants were transformed with the Arabidopsis CWM1 gene.HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with100.00%CompleteRestored32616665
Col-0P. patens PPR79Transformation and ComplementationExpressing the original PPR79 coding sequence in the cwm1 background.HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with51.00%MediumDecreased32616665
Col-0P. patens PPR79△DYWTransformation and ComplementationExpressing the original PPR79 coding sequence lacking the C-terminal DYW domain in the cwm1 background.HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with70.00%HighDecreased32616665
Col-0CWM1-DYWPPR79Transformation and ComplementationAdding the DYW domain from PPR79 after the PG-Box of CWM1 (CWM1-DYWPPR79), expressed in the cwm1 background.HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with100.00%CompleteRestored32616665
Col-0CWM1PLS-E1E2DYWPPR79Transformation and ComplementationReplacing the complete set of C-terminal domains from CWM1 with those from PPR79 (CWM1PLS-E1E2DYWPPR79), expressed in the cwm1 background.HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with100.00%CompleteRestored32616665
NAAt cwm1 ko complementation Line 1Transformation and ComplementationCWM1 targets transgenic lines in the Arabidopsis cwm1 mutant expressing Macadamia CWM1,HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with100.00%CompleteRestored32616665
NAAt cwm1 ko complementation Line 2Transformation and ComplementationCWM1 targets transgenic lines in the Arabidopsis cwm1 mutant expressing Macadamia CWM1,HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with71.00%HighDecreased32616665
Arabidopsis thaliana AT1G17630 Mitochondrion nad5 609 CDS C-to-U GUC=>GUU V=>V Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTNo treatmentNo treatmentNo treatmentNormalLeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with86.00%HighNone32616665
SALK_017325Ccwm1-1A T-DNA InsertionNAHomozygousA mild root growth phenotype and reduced seed germination rates on soil.Leaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with27.00%LowDecreased32616665
SALK_124160cwm1-2A T-DNA InsertionNAHomozygousA mild root growth phenotype and reduced seed germination rates on soil.Leaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with25.00%LowDecreased32616665
Col-0A. thaliana CWM1Transformation and ComplementationThe Arabidopsis cwm1 mutants were transformed with the Arabidopsis CWM1 gene.HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with73.00%HighDecreased32616665
Col-0P. patens PPR79Transformation and ComplementationExpressing the original PPR79 coding sequence in the cwm1 background.HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with44.00%MediumDecreased32616665
Col-0P. patens PPR79△DYWTransformation and ComplementationExpressing the original PPR79 coding sequence lacking the C-terminal DYW domain in the cwm1 background.HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with29.00%LowDecreased32616665
Col-0CWM1-DYWPPR79Transformation and ComplementationAdding the DYW domain from PPR79 after the PG-Box of CWM1 (CWM1-DYWPPR79), expressed in the cwm1 background.HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with73.00%HighDecreased32616665
Col-0CWM1PLS-E1E2DYWPPR79Transformation and ComplementationReplacing the complete set of C-terminal domains from CWM1 with those from PPR79 (CWM1PLS-E1E2DYWPPR79), expressed in the cwm1 background.HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with57.00%MediumDecreased32616665
NAAt cwm1 ko complementation Line 1Transformation and ComplementationCWM1 targets transgenic lines in the Arabidopsis cwm1 mutant expressing Macadamia CWM1,HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with100.00%CompleteRestored32616665
NAAt cwm1 ko complementation Line 2Transformation and ComplementationCWM1 targets transgenic lines in the Arabidopsis cwm1 mutant expressing Macadamia CWM1,HomozygousNALeaf from the rosette2- to 3-week oldcDNA sequencing and performing peak height calculations with64.00%HighDecreased32616665
Macadamia integrifolia NA Mitochondrion ccmB 428 CDS C-to-U UCG=>UUG S=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAM. integrifolia CWM1No treatmentNo treatmentNo treatmentNANANAcDNA sequencing and performing peak height calculations with41.00%MediumNone32616665
Macadamia integrifolia NA Mitochondrion ccmC 463 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAM. integrifolia CWM1No treatmentNo treatmentNo treatmentNANANAcDNA sequencing and performing peak height calculations with100.00%CompleteNone32616665
Macadamia integrifolia NA Mitochondrion nad5 598 CDS C-to-U CGU=>UGU R=>C Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAM. integrifolia CWM1No treatmentNo treatmentNo treatmentNANANAcDNA sequencing and performing peak height calculations with100.00%CompleteNone32616665
Macadamia integrifolia NA Mitochondrion nad5 609 CDS C-to-U GUC=>GUU V=>V Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
NAM. integrifolia CWM1No treatmentNo treatmentNo treatmentNANANAcDNA sequencing and performing peak height calculations with0.00%UneditedNone32616665
Last update: Jul 2021 (version 1.0)