Plant Editosome Database
a curated database of RNA editosome in plants

CWM2 - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: CWM2
Synonym: RRD2
Description: In addition to mutations in CELLULOSE SYNTHASE1 (CESA1) and CESA3, a forward genetic screen identified two independent defective genes encoding pentatricopeptide repeat (PPR)-like proteins (CELL WALL MAINTAINER1 [CWM1] and CWM2) as conferring tolerance to C17; Functional analysis revealed that mutations in these PPR proteins resulted in defective cytochrome c maturation and activation of mitochondrial retrograde signaling, as evidenced by the induction of an alternative oxidase; These mitochondrial perturbations increased tolerance to cell wall damage induced by cellulose deficiency; The C17 tolerance of cwm2 was partially lost upon depletion of the mitochondrial retrograde regulator ANAC017, demonstrating that ANAC017 links mitochondrial dysfunction with the cell wall
Protein Family: PPR
Subclass: NA
Construct Structure: NA
Gene ID & Species: AT1G32415 (Arabidopsis thaliana)
Edited Gene(s): ccmC
Editing Type(s): C-to-U (3)
Publication(s): [1] Mitochondrial Defects Confer Tolerance against Cellulose Deficiency., The Plant cell, 2016. [PMID=27543091]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT1G32415 Mitochondrion ccmC 575 CDS C-to-U CCC=>CUC P=>L Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
Col-0WTControlControlControlNormalRoot5-day-oldRT-PCR and direct sequencing93.33%HighNone27543091
SALK_020569Ccwm2-1T-DNA insertionThe cwm2-1 locus was mapped into the 171.8-kb region between SSLP markers CH1_11687 and CH1_11589NAHigher respiration than the wild type;C17 toleranceRoot5-day-oldRT-PCR and direct sequencing28.57%LowDecreased27543091
SALK_027874cwm2-2T-DNA insertionThe cwm2-2 locus was mapped into the 171.8-kb region between SSLP markers CH1_11687 and CH1_11589KnockoutHigher respiration than the wild type;C17 toleranceRoot5-day-oldRT-PCR and direct sequencing39.39%LowDecreased27543091
Last update: Jul 2021 (version 1.0)