CWM2 - Plant Editosome Database

Editing Factor:	CWM2
Synonym:	RRD2; CELL WALL MAINTAINER 2
Description:	In addition to mutations in CELLULOSE SYNTHASE1 (CESA1) and CESA3, a forward genetic screen identified two independent defective genes encoding pentatricopeptide repeat (PPR)-like proteins (CELL WALL MAINTAINER1 [CWM1] and CWM2) as conferring tolerance to C17; Functional analysis revealed that mutations in these PPR proteins resulted in defective cytochrome c maturation and activation of mitochondrial retrograde signaling, as evidenced by the induction of an alternative oxidase; These mitochondrial perturbations increased tolerance to cell wall damage induced by cellulose deficiency; The C17 tolerance of cwm2 was partially lost upon depletion of the mitochondrial retrograde regulator ANAC017, demonstrating that ANAC017 links mitochondrial dysfunction with the cell wall
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	E+
Physical Interaction:	NA
Construct Structure:	PLS-E1-E2-E+
Gene ID & Species:	AT1G32415 (Arabidopsis thaliana)
Edited Gene(s):	ccmC
Editing Type(s):	C-to-U (3)
Publication(s):	[1] Mitochondrial Defects Confer Tolerance against Cellulose Deficiency, Plant Cell, 2016. [doi:10.1105/tpc.16.00540]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT1G32415

Mitochondrion

575

CDS

Arabidopsis Genome Initiative

C-to-U

CCC=>CUC

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Root	5-day-old	RT-PCR and direct sequencing	93.33%	High	None	27543091
SALK_020569C	cwm2-1	T-DNA insertion	The cwm2-1 locus was mapped into the 171.8-kb region between SSLP markers CH1_11687 and CH1_11589	NA	Higher respiration than the wild type;C17 tolerance	Root	5-day-old	RT-PCR and direct sequencing	28.57%	Low	Decreased	27543091
SALK_027874	cwm2-2	T-DNA insertion	The cwm2-2 locus was mapped into the 171.8-kb region between SSLP markers CH1_11687 and CH1_11589	Knockout	Higher respiration than the wild type;C17 tolerance	Root	5-day-old	RT-PCR and direct sequencing	39.39%	Low	Decreased	27543091