| Ye478; Zheng58 | dek605-ds | A spontaneous mutation | Sequence analysis of the 11 candidate genes revealed a 414-bp Dissociation (Ds) element inserted at 1,011 bp downstream (from start codon) of gene10 (G10: GRMZM2G114241) in dek605-ds. | Recessive; Heterozygous | At maturity, dek605-ds seeds were thinner, shrunken and floury compared to corresponding WT, with decreased kernel weight; the 100-seed weight of dek605-ds was reduced by 30% compared to WT. dek605-ds exhibited a retarded embryo development during the whole growth stages observed between 14 to 20 DAP. While embryo structures, such as scutellum (SC), shoot apical meristem (SAM), root apical meristem (RAM) and leaf primordia (LP) were well established in WT, dek605-ds was delayed in development at the differentiation stage of plantule and radical at 14 DAP, longitudinal sections of dek605-ds of embryo and endosperm at 14 DAP showed structurally irregular cells in the basal endosperm transfer layer (BETL), compared to well-developed BETL-cells in WT. In addition, dek605-ds endosperm texture was softer, and starch granules were loosely arranged compared to WT. | Seed | 12 DAP | RT-PCR and chromatograms of cDNA sequencing | 0.00% | Unedited | Absent | 32818255 |
| B73; Chang7-2 (C7-2) | dek605-de | Deletion | Sequence analysis of the 0.78-Mb interval (between InDel140 and InDelM3) in dek605-de showed a large deletion from 171.5 Mb to 172.3 Mb (approximately 0.78 Mb), including the candidate gene G10. | Recessive; Heterozygous | Seeds and seedling development of another allele dek605-de, which is similar to dek605-ds, except that embryo development is less affected and more opaque endosperm compared to WT. | Seed | 12 DAP | RT-PCR and chromatograms of cDNA sequencing | 0.00% | Unedited | Absent | 32818255 |