Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Col-0 | dyw1-1 | EMS Mutant | The EMS mutant causeds a deletion of a guanidine at the position + 262 resulting a frameshift in the ORF | Knockout; Homozygous | A macroscopic phenotype identical to wild-type Col-0 plants; impaired in NDH Activity | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | 0.00% | Unedited | Absent | 23001034 |
| Col-0 | WT | Control | Control | Control | Normal | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | NA | High | None | 23001034 |
| NA | dyw1-1 | NA | NA | NA | Exhibit a superficially WT phenotype | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | 0.00% | Unedited | Absent | 25041347 |
| NA | dyw1-10 | EMS Mutant | The EMS mutant causes an amino acid change (R136K) in the short remnant E domain | Homozygous | Exhibit a superficially WT phenotype | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | NA | NA | Similar | 25041347 |
| NA | dyw1-11 | EMS Mutant | The EMS mutant causes an amino acid change (A138T) in the DYW domain | Homozygous | Exhibit a superficially WT phenotype | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | 37.70% | Low | Decreased | 25041347 |
| NA | dyw1-11 com1 | Complementation | NA | NA | NA | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | 60.00% | High | Decreased | 25041347 |
| NA | dyw1-11 com2 | Complementation | NA | NA | NA | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | 61.00% | High | Decreased | 25041347 |
| NA | dyw1-12 | EMS Mutant | The EMS mutant causes an amino acid change (E143K) in the DYW domain | Homozygous | Exhibit a superficially WT phenotype | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | 43.40% | Medium | Decreased | 25041347 |
| NA | dyw1-12 comp1 | Complementation | NA | NA | NA | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | 60.00% | High | Decreased | 25041347 |
| NA | dyw1-12 comp2 | Complementation | NA | NA | NA | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | 63.00% | High | Decreased | 25041347 |
| NA | dyw1-13 | EMS Mutant | The EMS mutant causes an amino acid change (P178S) in the DYW domain | Homozygous | Exhibit a superficially WT phenotype | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | NA | NA | Similar | 25041347 |
| NA | dyw1-14 | EMS Mutant | The EMS mutant causes an amino acid change (H219Y) in the DYW domain | Homozygous | Exhibit a superficially WT phenotype | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | NA | NA | Similar | 25041347 |
| NA | dyw1-15 | EMS Mutant | The EMS mutant causes an amino acid change (D222N) in the DYW domain | Homozygous | Exhibit a superficially WT phenotype | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | NA | NA | Similar | 25041347 |
| NA | dyw1-16 | EMS Mutant | The EMS mutant causes an amino acid change (D229N) in the DYW domain | Homozygous | Exhibit a superficially WT phenotype | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | 39.30% | Low | Decreased | 25041347 |
| NA | dyw1-16 comp1 | Complementation | NA | NA | NA | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | 62.00% | High | Increased | 25041347 |
| NA | dyw1-16 comp1 | Complementation | NA | NA | NA | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | 62.00% | High | Increased | 25041347 |
| NA | dyw1-2 | EMS Mutant | The EMS mutant causes an amino acid change (A11T) In The Putative Organelle Targeting Sequence | Homozygous | Exhibit a superficially WT phenotype | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | 66.50% | High | Decreased | 25041347 |
| NA | dyw1-2 comp1 | Complementation | NA | NA | NA | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | 63.00% | High | Decreased | 25041347 |
| NA | dyw1-2 comp2 | Complementation | NA | NA | NA | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | 72.00% | High | Decreased | 25041347 |
| NA | dyw1-3 | EMS Mutant | The EMS mutant causes an amino acid change (G30R) In The N-Terminal Of The E And DYW Domains | Homozygous | Exhibit a superficially WT phenotype | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | NA | NA | Similar | 25041347 |
| NA | dyw1-4 | EMS Mutant | The EMS mutant causes an amino acid change (T50I) In The N-Terminal Of The E And DYW Domains | Homozygous | Exhibit a superficially WT phenotype | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | 43.70% | Medium | Decreased | 25041347 |
| NA | dyw1-4 comp1 | Complementation | NA | NA | NA | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | 57.00% | Medium | Increased | 25041347 |
| NA | dyw1-5 | EMS Mutant | The EMS mutant causes an amino acid change (R61K) In The N-Terminal Of The E And DYW Domains | Homozygous | Exhibit a superficially WT phenotype | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | NA | NA | Similar | 25041347 |
| NA | dyw1-6 | EMS Mutant | The EMS mutant causes an amino acid change (R70K) In The N-Terminal Of The E And DYW Domains | Homozygous | Exhibit a superficially WT phenotype | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | NA | NA | Similar | 25041347 |
| NA | dyw1-7 | EMS Mutant | The EMS mutant causes an amino acid change (G112E) In The N-Terminal Of The E And DYW Domains | Homozygous | Exhibit a superficially WT phenotype | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | NA | NA | Similar | 25041347 |
| NA | dyw1-8 | EMS Mutant | The EMS mutant causes an amino acid change (G132E) In The Short Remnant E Domain | Homozygous | Exhibit a superficially WT phenotype | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | NA | NA | Similar | 25041347 |
| NA | dyw1-9 | EMS Mutant | The EMS mutant causes an amino acid change (E134K) In The Short Remnant E Domain | Homozygous | Exhibit a superficially WT phenotype | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | NA | NA | Similar | 25041347 |
| NA | M1 | Targeted PCR Mutant,Constructs Were Expressed Under Control Of The Native Dyw1Promoter And Used To Complementation The Dyw1-1 Mutant | The mutant causes (E165A) in the CDA signature sequence | NA | NA | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | 0.00% | Unedited | Absent | 25041347 |
| NA | M2 | Targeted PCR Mutant,Constructs Were Expressed Under Control Of The Native Dyw1Promoter And Used To Complementation The Dyw1-1 Mutant | The mutant causes (H163A+E165A) in the CDA signature sequence | NA | NA | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | 0.00% | Unedited | Absent | 25041347 |
| NA | M3 | Targeted PCR Mutant,Constructs Were Expressed Under Control Of The Native Dyw1Promoter And Used To Complementation The Dyw1-1 Mutant | The mutant causes (C191A+C194A) in the CDA signature sequence | NA | NA | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | 0.00% | Unedited | Absent | 25041347 |
| NA | M4 | Targeted PCR Mutant,Constructs Were Expressed Under Control Of The Native Dyw1Promoter And Used To Complementation The Dyw1-1 Mutant | The mutant causes (D229A) in the DYW triplet | NA | NA | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | 26.70% | Low | Absent | 25041347 |
| NA | M5 | Targeted PCR Mutant,Constructs Were Expressed Under Control Of The Native Dyw1Promoter And Used To Complementation The Dyw1-1 Mutant | The mutant causes (Y230A) in the DYW triplet | NA | NA | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | 51.70% | Medium | Absent | 25041347 |
| NA | M6 | Targeted PCR Mutant,Constructs Were Expressed Under Control Of The Native Dyw1Promoter And Used To Complementation The Dyw1-1 Mutant | The mutant causes (W231A) in the DYW triplet | NA | NA | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | 0.00% | Unedited | Absent | 25041347 |
| NA | M7 | Targeted PCR Mutant,Constructs Were Expressed Under Control Of The Native Dyw1Promoter And Used To Complementation The Dyw1-1 Mutant | The mutant causes (△DYW Triplet) in the DYW triplet | NA | NA | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | 0.00% | Unedited | Absent | 25041347 |
| Col-0 | WT | Control | Control | Control | Normal | Leaf | 18 days | Poisoned Primer Extension (PPE) Assay | 56.00% | Medium | None | 25041347 |
| Col-0 | WT | Control | Control | Control | Normal | Leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 26.00% | Low | None | 28761003 |
| Col-0 | DYW2-GFP-OE1 | Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene | Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen | NA | Slow growth | Leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 37.00% | Low | Increased | 28761003 |
| Col-0 | DYW2-GFP-OE2 | Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene | Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen | NA | Slow growth | Leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 46.00% | Medium | Increased | 28761003 |