ELI1 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor: ELI1
Synonym: EDITING LACKING INSERTIONAL MUTANT 1
Description: The ELI1 DYW deaminase domain was expressed as a recombinant protein in Escherichia coli and was shown to bind two zinc atoms per polypeptide; Thus, the DYW deaminase domain binds a zinc metal ion, as expected for a cytidine deaminase, and is potentially the catalytic component of an editing complex; Genetic complementation experiments demonstrate that large portions of the DYW deaminase domain of ELI1 may be eliminated, but the truncated genes retain the ability to restore editing site conversion in a mutant plant
Protein Family: Pentatricopeptide repeat (PPR) protein
Subclass: DYW
Physical Interaction: NA
Construct Structure: PLS-E-E+-DYW
Gene ID & Species: AT4G37380 (Arabidopsis thaliana)
Edited Gene(s): ndhB
Editing Type(s): C-to-U (6)
Publication(s): [1] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [doi:10.1073/pnas.1705815114]
[2] Identification of Two Pentatricopeptide Repeat Genes Required for RNA Editing and Zinc Binding by C-terminal Cytidine Deaminase-like Domains, The Journal of Biological Chemistry, 2013. [doi:10.1074/jbc.M113.485755]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Nuclear Genome Organelle Genome Other Region Other Position Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT4G37380 Chloroplast ndhB 830 CDS NA NA NA NA C-to-U UCA=>UUA
NA=>NA
S=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
CS877857eli1-1T-DNA InsertionNANAMutant plants do not display a dramatic phenotype under growth chamber conditions NANABulk sequencingNAUneditedAbsent24194514
CS100280eli1-2T-DNA InsertionT-DNA is inserted in repeat 3NAMutant plants do not display a dramatic phenotype under growth chamber conditions NANABulk sequencingNAUneditedAbsent24194514
Col-0WTControlControlNo mutantNormalNANABulk sequencingNACompleteNone24194514
Col-0WTControlControlNo mutantNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract99.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract99.00%HighSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Last update: Sep 2026 (version 2.0)