EMP17 - Plant Editosome Database

Summary

Editing Factor:	EMP17
Synonym:	Empty Pericarp 17; PPR22; Pentatricopeptide Repeat Protein 22
Description:	As CcmFC functions in cytochrome c (Cytc) maturation, the amount of Cytc and Cytc 1 protein is drastically reduced in emp17, suggesting that the CcmFC-267 (Ser to Pro) change impairs the CcmFC function; As a result, the assembly of complex III is strikingly decreased in emp17; In contrast, the assembly of complex I appears less affected, suggesting that the Nad2-226 (Phe to Ser) change may have less impact on Nad2 function
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	DYW
Physical Interaction:	NA
Construct Structure:	PLS-E1-E2-DYW
Gene ID & Species:	GRMZM2G019689 (Zea mays)
Edited Gene(s):	ccmFc nad2
Editing Type(s):	C-to-U (16)
Publication(s):	[1] PPR-DYW Protein EMP17 Is Required for Mitochondrial RNA Editing, Complex III Biogenesis, and Seed Development in Maize, Front Plant Sci, 2021. [doi:10.3389/fpls.2021.693272]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Zea mays

GRMZM2G019689

Mitochondrion

ccmFc

799

CDS

B73

C-to-U

CCC=>UCC

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT	Control	Control	No mutant	Normal	Kernel	NA	Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	34394147
W22	emp17	An insertion mutant	A Mu3 element was confirmed to be inserted at +660 bp from the translation start codon of Emp17.	Heterozygous	Loss of the EMP17 function severely arrests embryogenesis and endosperm development in maize.	Kernel	NA	Direct sequencing of RT-PCR amplicons	0.00%	Unedited	Absent	34394147
B73	WT	Control	Control	No mutant	Normal	Seedling	NA	Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	34394147
W22	Emp17-OE1:emp17 (-/-)	Over-expressing Emp17	Transgenic plants over-expressing Emp17 (Emp17-OE) are created in the inbred line KN5585 by placing Emp17 under the Ubi-1 promoter.	Homozygous	Seedlings show normal growth and development compared with the wild type	Seedling	NA	Direct sequencing of RT-PCR amplicons	100.00%	Complete	Restored	34394147

Zea mays

GRMZM2G019689

Mitochondrion

ccmFc

906

CDS

B73

C-to-U

UCC=>UCU

S=>S

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT	Control	Control	No mutant	Normal	Kernel	NA	Direct sequencing of RT-PCR amplicons	77.19%	High	None	34394147
W22	emp17	An insertion mutant	A Mu3 element was confirmed to be inserted at +660 bp from the translation start codon of Emp17.	Heterozygous	Loss of the EMP17 function severely arrests embryogenesis and endosperm development in maize.	Kernel	NA	Direct sequencing of RT-PCR amplicons	50.00%	Medium	Decreased	34394147
B73	WT	Control	Control	No mutant	Normal	Seedling	NA	Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	34394147
W22	Emp17-OE1:emp17 (-/-)	Over-expressing Emp17	Transgenic plants over-expressing Emp17 (Emp17-OE) are created in the inbred line KN5585 by placing Emp17 under the Ubi-1 promoter.	Homozygous	Seedlings show normal growth and development compared with the wild type	Seedling	NA	Direct sequencing of RT-PCR amplicons	100.00%	Complete	Restored	34394147

Zea mays

GRMZM2G019689

Mitochondrion

ccmFc

966

CDS

B73

C-to-U

CCC=>CCU

P=>P

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT	Control	Control	No mutant	Normal	Kernel	NA	Direct sequencing of RT-PCR amplicons	77.78%	High	None	34394147
W22	emp17	An insertion mutant	A Mu3 element was confirmed to be inserted at +660 bp from the translation start codon of Emp17.	Heterozygous	Loss of the EMP17 function severely arrests embryogenesis and endosperm development in maize.	Kernel	NA	Direct sequencing of RT-PCR amplicons	58.46%	Medium	Decreased	34394147
B73	WT	Control	Control	No mutant	Normal	Seedling	NA	Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	34394147
W22	Emp17-OE1:emp17 (-/-)	Over-expressing Emp17	Transgenic plants over-expressing Emp17 (Emp17-OE) are created in the inbred line KN5585 by placing Emp17 under the Ubi-1 promoter.	Homozygous	Seedlings show normal growth and development compared with the wild type	Seedling	NA	Direct sequencing of RT-PCR amplicons	100.00%	Complete	Restored	34394147

Zea mays

GRMZM2G019689

Mitochondrion

nad2

677

CDS

B73

C-to-U

UCC=>UUC

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
B73	WT	Control	Control	No mutant	Normal	Kernel	NA	Direct sequencing of RT-PCR amplicons	87.88%	High	None	34394147
W22	emp17	An insertion mutant	A Mu3 element was confirmed to be inserted at +660 bp from the translation start codon of Emp17.	Heterozygous	Loss of the EMP17 function severely arrests embryogenesis and endosperm development in maize.	Kernel	NA	Direct sequencing of RT-PCR amplicons	0.00%	Unedited	Decreased	34394147
B73	WT	Control	Control	No mutant	Normal	Seedling	NA	Direct sequencing of RT-PCR amplicons	100.00%	Complete	None	34394147
W22	Emp17-OE1:emp17 (-/-)	Over-expressing Emp17	Transgenic plants over-expressing Emp17 (Emp17-OE) are created in the inbred line KN5585 by placing Emp17 under the Ubi-1 promoter.	Homozygous	Seedlings show normal growth and development compared with the wild type	Seedling	NA	Direct sequencing of RT-PCR amplicons	100.00%	Complete	Restored	34394147