Plant Editosome Database
a curated database of RNA editosome in plants

EMP17 - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: EMP17
Synonym: Empty Pericarp 17
Description: As CcmFC functions in cytochrome c (Cytc) maturation, the amount of Cytc and Cytc 1 protein is drastically reduced in emp17, suggesting that the CcmFC-267 (Ser to Pro) change impairs the CcmFC function; As a result, the assembly of complex III is strikingly decreased in emp17; In contrast, the assembly of complex I appears less affected, suggesting that the Nad2-226 (Phe to Ser) change may have less impact on Nad2 function
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E1-E2-DYW
Gene ID & Species: GRMZM2G019689 (Zea mays)
Edited Gene(s): ccmFc    nad2
Editing Type(s): C-to-U (16)
Publication(s): [1] PPR-DYW Protein EMP17 Is Required for Mitochondrial RNA Editing, Complex III Biogenesis, and Seed Development in Maize., Frontiers in plant science, 2021. [PMID=34394147]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Zea mays GRMZM2G019689 Mitochondrion ccmFc 799 CDS C-to-U CCC=>UCC P=>S Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernelNADirect sequencing of RT-PCR amplicons100.00%CompleteNone34394147
W22emp17An insertion mutantA Mu3 element was confirmed to be inserted at +660 bp from the translation start codon of Emp17.HeterozygousLoss of the EMP17 function severely arrests embryogenesis and endosperm development in maize.KernelNADirect sequencing of RT-PCR amplicons0.00%UneditedAbsent34394147
B73WTControlControlNo mutantNormalSeedlingNADirect sequencing of RT-PCR amplicons100.00%CompleteNone34394147
W22Emp17-OE1:emp17 (-/-)Over-expressing Emp17Transgenic plants over-expressing Emp17 (Emp17-OE) are created in the inbred line KN5585 by placing Emp17 under the Ubi-1 promoter.HomozygousSeedlings show normal growth and development compared with the wild typeSeedlingNADirect sequencing of RT-PCR amplicons100.00%CompleteRestored34394147
Zea mays GRMZM2G019689 Mitochondrion ccmFc 906 CDS C-to-U UCC=>UCU S=>S Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernelNADirect sequencing of RT-PCR amplicons77.19%HighNone34394147
W22emp17An insertion mutantA Mu3 element was confirmed to be inserted at +660 bp from the translation start codon of Emp17.HeterozygousLoss of the EMP17 function severely arrests embryogenesis and endosperm development in maize.KernelNADirect sequencing of RT-PCR amplicons50.00%MediumDecreased34394147
B73WTControlControlNo mutantNormalSeedlingNADirect sequencing of RT-PCR amplicons100.00%CompleteNone34394147
W22Emp17-OE1:emp17 (-/-)Over-expressing Emp17Transgenic plants over-expressing Emp17 (Emp17-OE) are created in the inbred line KN5585 by placing Emp17 under the Ubi-1 promoter.HomozygousSeedlings show normal growth and development compared with the wild typeSeedlingNADirect sequencing of RT-PCR amplicons100.00%CompleteRestored34394147
Zea mays GRMZM2G019689 Mitochondrion ccmFc 966 CDS C-to-U CCC=>CCU P=>P Synonymous
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernelNADirect sequencing of RT-PCR amplicons77.78%HighNone34394147
W22emp17An insertion mutantA Mu3 element was confirmed to be inserted at +660 bp from the translation start codon of Emp17.HeterozygousLoss of the EMP17 function severely arrests embryogenesis and endosperm development in maize.KernelNADirect sequencing of RT-PCR amplicons58.46%MediumDecreased34394147
B73WTControlControlNo mutantNormalSeedlingNADirect sequencing of RT-PCR amplicons100.00%CompleteNone34394147
W22Emp17-OE1:emp17 (-/-)Over-expressing Emp17Transgenic plants over-expressing Emp17 (Emp17-OE) are created in the inbred line KN5585 by placing Emp17 under the Ubi-1 promoter.HomozygousSeedlings show normal growth and development compared with the wild typeSeedlingNADirect sequencing of RT-PCR amplicons100.00%CompleteRestored34394147
Zea mays GRMZM2G019689 Mitochondrion nad2 677 CDS C-to-U UCC=>UUC S=>F Recoding
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
B73WTControlControlNo mutantNormalKernelNADirect sequencing of RT-PCR amplicons87.88%HighNone34394147
W22emp17An insertion mutantA Mu3 element was confirmed to be inserted at +660 bp from the translation start codon of Emp17.HeterozygousLoss of the EMP17 function severely arrests embryogenesis and endosperm development in maize.KernelNADirect sequencing of RT-PCR amplicons0.00%UneditedDecreased34394147
B73WTControlControlNo mutantNormalSeedlingNADirect sequencing of RT-PCR amplicons100.00%CompleteNone34394147
W22Emp17-OE1:emp17 (-/-)Over-expressing Emp17Transgenic plants over-expressing Emp17 (Emp17-OE) are created in the inbred line KN5585 by placing Emp17 under the Ubi-1 promoter.HomozygousSeedlings show normal growth and development compared with the wild typeSeedlingNADirect sequencing of RT-PCR amplicons100.00%CompleteRestored34394147
Last update: Jul 2021 (version 1.0)