Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| C24 | C24 | Control | Control | No mutant | Normal | NA | 3-4 weeks | Direct Sequencing of PCR Products | 30.00% | Low | Decreased | 19252080 |
| C24 | C24 protoplasts | Control | Control | No mutant | Normal | Protoplast | 3-4 weeks | Direct Sequencing of PCR Products | 15.00% | Poor | Decreased | 19252080 |
| C24 | C24 protoplasts + GFP | Transformation | 35S:GFP is introduced into C24 protoplasts | NA | NA | Protoplast | 3-4 weeks | Direct Sequencing of PCR Products | 20.00% | Low | Restored | 19252080 |
| C24 | C24 protoplasts + Col MEF1 | Transformation | Col version of gene MEF1 (35S:MEF1 sequence traces) is introduced into C24 protoplasts | NA | NA | Protoplast | 3-4 weeks | Direct Sequencing of PCR Products | 100.00% | Complete | Restored | 19252080 |
| Col-0 | Col-0 | Control | Control | No mutant | Normal | NA | 3-4 weeks | Direct Sequencing of PCR Products | 100.00% | Complete | None | 19252080 |
| Col-0 | Col protoplasts | Control | Control | No mutant | Normal | Protoplast | 3-4 weeks | Direct Sequencing of PCR Products | 100.00% | Complete | None | 19252080 |
| Ler | Ler | Control | Control | No mutant | Normal | NA | 3-4 weeks | Direct Sequencing of PCR Products | 100.00% | Complete | None | 19252080 |
| Col-0 | mef1-1 | The Single Mutation | Gly To Glu in the C-terminal L motif in one of the 35 amino acids repeats | Homozygous | Normal growth phenotype | Protoplast | 3-4 weeks | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 19252080 |
| Col-0 | mef1-1 + MEF1 | Complementation | The Col wild-type MEF1 gene is transfected into mef1-1 | NA | NA | Protoplast | 3-4 weeks | Direct Sequencing of PCR Products | 55.00% | Medium | Increased | 19252080 |
| Col-0 | mef1-2 | The Single Mutation | Ser to Phe in the DYW domain | NA | Normal growth phenotype | Protoplast | 3-4 weeks | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 19252080 |
| Col-0 | mef1-2 + MEF1 | Complementation | The Col wild-type MEF1 gene is transfected into mef1-2 | NA | NA | Protoplast | 3-4 weeks | Direct Sequencing of PCR Products | 65.00% | High | Increased | 19252080 |
| Col-0 | WT | Control | Control | No mutant | Normal | Leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 99.00% | High | None | 28761003 |
| Col-0 | DYW2-GFP-OE1 | Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene | Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen | NA | Slow growth | Leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 100.00% | Complete | Similar | 28761003 |
| Col-0 | DYW2-GFP-OE2 | Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene | Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen | NA | Slow growth | Leaf | NA | RT-PCR and calculated with the DNADynamo software (BlueTract | 100.00% | Complete | Similar | 28761003 |
| Col-0 | Untransfected mef1-1 protoplasts | Mutation | An polymorphism in an P motif (nucleotide position 1009) changes G to A (amino acid G to E) | NA | NA | Protoplast | 3-4-week-old plantlet | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 20828567 |
| Col-0 | (+ MEF1 Col) | Mutation and transformation | Transfecting mutant protoplasts with the Col wild-type MEF1 | NA | NA | Protoplast | 3-4-week-old plantlet | Direct Sequencing of PCR Products | 50.00% | Medium | Restored | 20828567 |
| C24 | (+ MEF1 C24-1) | Mutation and transformation | Transfecting mutant protoplasts with the construct of the Col-MEF1 gene mutated at the variant C24 nucleotide positions 214 | NA | NA | Protoplast | 3-4-week-old plantlet | Direct Sequencing of PCR Products | 55.00% | Medium | Restored | 20828567 |
| C24 | (+ MEF1 C24-2) | Mutation and transformation | Transfecting mutant protoplasts with the construct of the Col-MEF1 gene mutated at the variant C24 nucleotide positions 314 | NA | NA | Protoplast | 3-4-week-old plantlet | Direct Sequencing of PCR Products | 50.00% | Medium | Restored | 20828567 |
| C24 | (+ MEF1 C24-3) | Mutation and transformation | Transfecting mutant protoplasts with the construct of the Col-MEF1 gene mutated at the variant C24 nucleotide positions 1297 | NA | NA | Protoplast | 3-4-week-old plantlet | Direct Sequencing of PCR Products | 45.00% | Medium | Restored | 20828567 |
| Col-0 | Untransfected mef1-2 protoplasts | Mutation | An polymorphism in the DYW domain (nucleotide position 1676) changes C to T (amino acid S to F) | NA | NA | Protoplast | 3-4-week-old plantlet | Direct Sequencing of PCR Products | 0.00% | Unedited | Absent | 20828567 |
| Col-0 | (+ MEF1 Col) | Mutation and transformation | Transfecting mutant protoplasts with the Col wild-type MEF1 | NA | NA | Protoplast | 3-4-week-old plantlet | Direct Sequencing of PCR Products | 50.00% | Medium | Restored | 20828567 |
| C24 | (+ MEF1 C24-1) | Mutation and transformation | Transfecting mutant protoplasts with the construct of the Col-MEF1 gene mutated at the variant C24 nucleotide positions 214 | NA | NA | Protoplast | 3-4-week-old plantlet | Direct Sequencing of PCR Products | 60.00% | High | Restored | 20828567 |
| C24 | (+ MEF1 C24-2) | Mutation and transformation | Transfecting mutant protoplasts with the construct of the Col-MEF1 gene mutated at the variant C24 nucleotide positions 314 | NA | NA | Protoplast | 3-4-week-old plantlet | Direct Sequencing of PCR Products | 50.00% | Medium | Restored | 20828567 |
| C24 | (+ MEF1 C24-3) | Mutation and transformation | Transfecting mutant protoplasts with the construct of the Col-MEF1 gene mutated at the variant C24 nucleotide positions 1297 | NA | NA | Protoplast | 3-4-week-old plantlet | Direct Sequencing of PCR Products | 50.00% | Medium | Restored | 20828567 |
| C24 | (+ MEF1 C24-1+2) | Mutation and transformation | An polymorphism in an P motif (nucleotide position 1009) changes G to A (amino acid G to E); Transfecting mutant protoplasts with the construct of the Col-MEF1 gene mutated at the variant C24 nucleoti | NA | NA | Protoplast | 3-4-week-old plantlet | Direct Sequencing of PCR Products | 50.00% | Medium | Restored | 20828567 |
| C24 | (+ MEF1 C24-1+3) | Mutation and transformation | An polymorphism in an P motif (nucleotide position 1009) changes G to A (amino acid G to E); Transfecting mutant protoplasts with the construct of the Col-MEF1 gene mutated at the variant C24 nucleoti | NA | NA | Protoplast | 3-4-week-old plantlet | Direct Sequencing of PCR Products | 50.00% | Medium | Restored | 20828567 |
| C24 | (+ MEF1 C24-2+3) | Mutation and transformation | An polymorphism in an P motif (nucleotide position 1009) changes G to A (amino acid G to E); Transfecting mutant protoplasts with the construct of the Col-MEF1 gene mutated at the variant C24 nucleoti | NA | NA | Protoplast | 3-4-week-old plantlet | Direct Sequencing of PCR Products | 35.00% | Low | Decreased | 20828567 |
| C24 | (+ MEF1 C24-1+2+3) | Mutation and transformation | An polymorphism in an P motif (nucleotide position 1009) changes G to A (amino acid G to E); Transfecting mutant protoplasts with the construct of the Col-MEF1 gene mutated at the variant C24 nucleoti | NA | NA | Protoplast | 3-4-week-old plantlet | Direct Sequencing of PCR Products | 55.00% | Medium | Restored | 20828567 |
| C24 | (+ MEF1 C24-1+2) | Mutation and transformation | An polymorphism in the DYW domain (nucleotide position 1676) changes C to T (amino acid S to F); Transfecting mutant protoplasts with the construct of the Col-MEF1 gene mutated at the variant C24 nucl | NA | NA | Protoplast | 3-4-week-old plantlet | Direct Sequencing of PCR Products | 50.00% | Medium | Restored | 20828567 |
| C24 | (+ MEF1 C24-1+3) | Mutation and transformation | An polymorphism in the DYW domain (nucleotide position 1676) changes C to T (amino acid S to F); Transfecting mutant protoplasts with the construct of the Col-MEF1 gene mutated at the variant C24 nucl | NA | NA | Protoplast | 3-4-week-old plantlet | Direct Sequencing of PCR Products | 50.00% | Medium | Restored | 20828567 |
| C24 | (+ MEF1 C24-2+3) | Mutation and transformation | An polymorphism in the DYW domain (nucleotide position 1676) changes C to T (amino acid S to F); Transfecting mutant protoplasts with the construct of the Col-MEF1 gene mutated at the variant C24 nucl | NA | NA | Protoplast | 3-4-week-old plantlet | Direct Sequencing of PCR Products | 30.00% | Low | Decreased | 20828567 |
| C24 | (+ MEF1 C24-1+2+3) | Mutation and transformation | An polymorphism in the DYW domain (nucleotide position 1676) changes C to T (amino acid S to F); Transfecting mutant protoplasts with the construct of the Col-MEF1 gene mutated at the variant C24 nucl | NA | NA | Protoplast | 3-4-week-old plantlet | Direct Sequencing of PCR Products | 40.00% | Medium | Decreased | 20828567 |
