MEF10 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: MEF10
Synonym: MITOCHONDRIAL RNA EDITING FACTOR 10
Description: Encoding a protein required for RNA editing at nad2-842 in mitochondria of Arabidopsis thaliana and interacting with MORF8
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E-E+-DYW
Gene ID & Species: AT3G11460 (Arabidopsis thaliana)
Edited Gene(s): nad2
Editing Type(s): C-to-U (7)
Publication(s): [1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003]
[3] MEF10 is required for RNA editing at nad2-842 in mitochondria of Arabidopsis thaliana and interacts with MORF8., Plant molecular biology, 2013. [PMID=23288601]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT3G11460 Mitochondrion nad2 842 CDS C-to-U UCU=>UUU
NA=>NA
S=>F
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products100.00%CompleteNA23288601
Colmef10-1EMS MutantC to T (G to A on the other strand) transition at nucleotide 1333 causes an Ala to Thr amino acid change at residue 445HomozygousGrow slowerLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent23288601
SAIL_599_BO1mef10-2T-DNA InsertionThe T-DNA is inserted in the 5'-transcribed but untranslated region of MEF10(a T-DNA insert in the 50-UTR of the MEF10 gene)NAGrow normallyLeafNADirect Sequencing of PCR Products60.00%HighDecreased23288601
Col-0WTControlControlControlNANANARNA-seq98.47%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Last update: Jul 2021 (version 1.0)