MEF10 - Plant Editosome Database

Summary

Editing Factor:	MEF10
Synonym:	MITOCHONDRIAL RNA EDITING FACTOR 10
Description:	Encoding a protein required for RNA editing at nad2-842 in mitochondria of Arabidopsis thaliana and interacting with MORF8
Protein Family:	PPR
Subclass:	DYW
Physical Interaction:	MORF8
Construct Structure:	PLS-E-E+-DYW
Gene ID & Species:	AT3G11460 (Arabidopsis thaliana)
Edited Gene(s):	nad2
Editing Type(s):	C-to-U (7)
Publication(s):	[1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170] [2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003] [3] MEF10 is required for RNA editing at nad2-842 in mitochondria of Arabidopsis thaliana and interacts with MORF8., Plant molecular biology, 2013. [PMID=23288601]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT3G11460

Mitochondrion

nad2

842

CDS

NA
EnsemblGenomes (release 39)

NA
BK010421

C-to-U

UCU=>UUU
NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col	WT	Control	Control	Control	Normal	Leaf	NA	Direct Sequencing of PCR Products	100.00%	Complete	NA	23288601
Col	mef10-1	EMS Mutant	C to T (G to A on the other strand) transition at nucleotide 1333 causes an Ala to Thr amino acid change at residue 445	Homozygous	Grow slower	Leaf	NA	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	23288601
SAIL_599_BO1	mef10-2	T-DNA Insertion	The T-DNA is inserted in the 5'-transcribed but untranslated region of MEF10(a T-DNA insert in the 50-UTR of the MEF10 gene)	NA	Grow normally	Leaf	NA	Direct Sequencing of PCR Products	60.00%	High	Decreased	23288601
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	98.47%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003