MEF10 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: MEF10
Synonym: MITOCHONDRIAL RNA EDITING FACTOR 10
Description: Encodes a protein required for RNA editing at nad2-842 in mitochondria of Arabidopsis thaliana and interacting with MORF8.
Protein Family: PPR
Subclass: DYW
Construct Structure: PLS-E-E+-DYW
Gene ID & Species: AT3G11460 (Arabidopsis thaliana)
Edited Gene(s): nad2
Editing Type(s): C-to-U (7)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003]
[3] MEF10 is required for RNA editing at nad2-842 in mitochondria of Arabidopsis thaliana and interacts with MORF8, Plant Molecular Biology, 2013. [PMID=23288601]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT3G11460 Mitochondrion nad2 842 CDS C-to-U UCU=>UUU
NA=>NA
S=>F
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNADirect Sequencing of PCR Products100.00%CompleteNA23288601
Colmef10-1EMS MutantC to T(G to A on the other strand) transition at nucleotide 1333 causes an Ala to Thr amino acid change at residue 445HomozygousGrow slowerLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent23288601
SAIL_599_BO1mef10-2T-DNA InsertionThe T-DNA is inserted in the 5'-transcribed but untranslated region of MEF10(a T-DNA insert in the 50-UTR of the MEF10 gene)NAGrow normallyLeafNADirect Sequencing of PCR Products60.00%MediumDecreased23288601
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq98.47%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Last update: Jul 2021 (version 1.0)