MEF12 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: MEF12
Synonym: MITOCHONDRIAL RNA EDITING FACTOR 12
Description: Encoding a site-specifc mitochondrial RNA editing factor with a long stretch of 25 pentatricopeptide repeats extended by an E domain in Arabidopsis
Protein Family: PPR
Subclass: E,E+
Construct Structure: PLS-E, NA
Gene ID & Species: AT3G09040 (Arabidopsis thaliana)
Edited Gene(s): nad5
Editing Type(s): C-to-U (7)
Publication(s): [1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003]
[3] The longest mitochondrial RNA editing PPR protein MEF12 in Arabidopsis thaliana requires the full-length E domain., RNA biology, 2013. [PMID=23845994]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT3G09040 Mitochondrion nad5 374 CDS C-to-U CCA=>CUA
NA=>NA
P=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColWTControlControlControlNormalLeafNASpecifc cDNA sequence analysis100.00%CompleteNone23845994
Colmef12-1EMS MutantThe G to A transition at nucleotide 2925 causes the change of a UGG trytophan codon to a UGA translational stop in the mef12-1 mutant plant line.HomozygousNo phenotypic defectLeafNASpecifc cDNA sequence analysis0.00%UneditedAbsent23845994
FLAG_394H02mef12-2T-DNA InsertionT-DNA is inserted in the E domain regionHomozygousNo phenotypic defectLeafNASpecifc cDNA sequence analysis20.00%LowDecreased23845994
Col-0WTControlControlControlNANANARNA-seq98.89%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract99.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract95.00%HighSimilar28761003
Last update: Jul 2021 (version 1.0)