MEF12 - Plant Editosome Database

Summary

Editing Factor:	MEF12
Synonym:	MITOCHONDRIAL RNA EDITING FACTOR 12
Description:	The MEF12 polypeptide is characterized by an exceptionally long stretch of 25 pentatricopeptide repeats (PPR) and a C-terminal extension domain; Editing is lost in mutant plants with a stop codon in the extending element; A T-DNA insertion substituting the 10 C-terminal amino acids of the extension domain reduces RNA editing to about 20% at the target site in a mutant plant; These results support the importance of the full-length extension module for functional RNA editing in plant mitochondria
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	E
Physical Interaction:	NA
Construct Structure:	PLS-E
Gene ID & Species:	AT3G09040 (Arabidopsis thaliana)
Edited Gene(s):	nad5
Editing Type(s):	C-to-U (6)
Publication(s):	[1] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [doi:10.1073/pnas.1705815114] [2] The longest mitochondrial RNA editing PPR protein MEF12 in Arabidopsis thaliana requires the full-length E domain, RNA Biology, 2013. [doi:10.4161/rna.25484]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT3G09040

Mitochondrion

nad5

374

CDS

C-to-U

CCA=>CUA
NA=>NA

P=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col	WT	Control	Control	No mutant	Normal	Leaf	NA	Specifc cDNA sequence analysis	100.00%	Complete	None	23845994
Col	mef12-1	EMS Mutant	The G to A transition at nucleotide 2925 causes the change of a UGG trytophan codon to a UGA translational stop in the mef12-1 mutant plant line.	Homozygous	No phenotypic defect	Leaf	NA	Specifc cDNA sequence analysis	0.00%	Unedited	Absent	23845994
FLAG_394H02	mef12-2	T-DNA Insertion	T-DNA is inserted in the E domain region	Homozygous	No phenotypic defect	Leaf	NA	Specifc cDNA sequence analysis	20.00%	Low	Decreased	23845994
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	99.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	95.00%	High	Similar	28761003