MEF12 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: MEF12
Synonym: MITOCHONDRIAL RNA EDITING FACTOR 12
Description: Encodes a site-specifc mitochondrial RNA editing factor with a long stretch of 25 pentatricopeptide repeats (PPR) extended by an E domain in Arabidopsis.
Protein Family: PPR
Subclass: E,E+
Construct Structure: PLS-E, NA
Gene ID & Species: AT3G09040 (Arabidopsis thaliana)
Edited Gene(s): nad5
Editing Type(s): C-to-U (7)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003]
[3] The longest mitochondrial RNA editing PPR protein MEF12 in Arabidopsis thaliana requires the full-length E domain, RNA Biology, 2013. [PMID=23845994]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT3G09040 Mitochondrion nad5 374 CDS C-to-U CCA=>CUA
NA=>NA
P=>L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColWTNo treatmentNo treatmentNo treatmentNo treatmentLeafNASpecifc cDNA sequence analysis100.00%CompleteNone23845994
Colmef12-1EMS MutantThe G to A transition at nucleotide 2925 (arrow) causes the change of a UGG trytophan codon to a UGA translational stop in the mef12-1 mutant plant line.HomozygousNo phenotypic defectLeafNASpecifc cDNA sequence analysis0.00%UneditedAbsent23845994
FLAG_394H02mef12-2T-DNA InsertionT-DNA is inserted in the E domain regionHomozygousNo phenotypic defectLeafNASpecifc cDNA sequence analysis20.00%LowDecreased23845994
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq98.89%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract99.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract95.00%HighSimilar28761003
Last update: Jul 2021 (version 1.0)