MEF25 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor: MEF25
Synonym: MITOCHONDRIAL RNA EDITING FACTOR 25
Description: Encoding a pentatricopeptide repeat protein essential for RNA editing at the nad1-308 site in Arabidopsis mitochondria
Protein Family: PPR
Subclass: E+
Construct Structure: PLS-E-E+
Gene ID & Species: AT3G25060 (Arabidopsis thaliana)
Edited Gene(s): nad1
Editing Type(s): C-to-U (7)
Publication(s): [1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170]
[2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003]
[3] Contiguous RNA editing sites in the mitochondrial nad1 transcript of Arabidopsis thaliana are recognized by different proteins., FEBS letters, 2013. [PMID=23416303]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT3G25060 Mitochondrion nad1 308 CDS C-to-U CCG/CCG=>CUG/UUG
NA=>NA
P=>L/L
NA=>NA
Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColWTControlControlControlNormalLeafNADirect Sequencing of PCR Products97.60%HighNA23416303
SAIL 672mef25-1T-DNA InsertionThe T-DNA inserstion causes a 135 bp deletion (between +641 and +775) and a 9 bp insertionHomozygousNo changes were seenLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent23416303
SALK_091381Cmef25-2T-DNA InsertionThe T-DNA inserstion causes a 49 bp deletion (between +936 and +984) and two insertions (8 and 5 bp)HomozygousNo changes were seenLeafNADirect Sequencing of PCR Products0.00%UneditedAbsent23416303
Col-0WTControlControlControlNANANARNA-seq95.21%HighNone32098170
Col-0WTControlControlControlNormalLeafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract20.00%LowDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthLeafNART-PCR and calculated with the DNADynamo software (BlueTract19.00%PoorDecreased28761003
Last update: Jul 2021 (version 1.0)