MEF31 - Plant Editosome Database - CNCB-NGDC

MEF31 - Plant Editosome Database - CNCB-NGDC

Summary

Editing Factor:	MEF31
Synonym:	MITOCHONDRIAL EDITING FACTOR 31
Description:	Mitochondrial editing factor 31 (MEF31), an E-PPR protein involved in editing at two close sites in the same transcript encoding subunit C of the twin-arginine translocation (tat) pathway; MEF31 is essential for editing at site tatC-581 and application of the recently proposed amino acid code for RNA recognition by PPR proteins supports the view that MEF31 directly targets this site by recognizing its cis sequence; In contrast, editing at site tatC-586 five nucleotides downstream is only partially affected in plants lacking MEF31, being restored to wild-type levels in complemented plants; Application of the amino acid code and analysis of individual RNA molecules for editing at sites 581 and 586 suggest that MEF31 does not directly target site tatC-586, and only indirectly influences editing at this site
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	E
Physical Interaction:	NA
Construct Structure:	PLS-E
Gene ID & Species:	AT2G46050 (Arabidopsis thaliana)
Edited Gene(s):	tatC
Editing Type(s):	C-to-U (4)
Publication(s):	[1] The Pentatricopeptide Repeat Protein MEF31 is Required for Editing at Site 581 of the Mitochondrial tatC Transcript and Indirectly Influences Editing at Site 586 of the Same Transcript, Plant Cell Physiol, 2018. [doi:10.1093/pcp/pcx190]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT2G46050

Mitochondrion

581

CDS

NA

NA

NA

NA

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	No mutant	Normal	Leaf	4 weeks	Direct sequence analysis of RT-PCR products	100.00%	Complete	None	29216369
SAIL_451_B04;CS820747	mef31-1	T-DNA insertion	The insertion in the mutant is located in the sixth PPR motif	Homozygous	No phenotypic alterations were observed during growth of mef31 mutants when compared with wild-type plants, at least under standard growth conditions	Leaf	4 weeks	Direct sequence analysis of RT-PCR products	0.00%	Unedited	Absent	29216369
SALK_146433	mef31-2	T-DNA insertion	The insertion in the mutant is located in the ninth PPR motif	Homozygous	No phenotypic alterations were observed during growth of mef32 mutants when compared with wild-type plants, at least under standard growth conditions	Leaf	4 weeks	Direct sequence analysis of RT-PCR products	0.00%	Unedited	Absent	29216369
SAIL_451_B04;CS820747	mef31-1/35S::MEF31	T-DNA insertion;Complemented	The insertion in the mutant is located in the sixth PPR motif;mef31-1 plants transformed with the wild-type MEF31 gene	Homozygous	No phenotypic alterations were observed during growth of mef33 mutants when compared with wild-type plants, at least under standard growth conditions	Leaf	4 weeks	Direct sequence analysis of RT-PCR products	80.00%	High	Restored	29216369

Last update: Sep 2026 (version 2.0)