MEF31 - Plant Editosome Database - BIG Data Center

MEF31 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor:	MEF31
Synonym:	MITOCHONDRIAL EDITING FACTOR 31
Description:	E-PPR protein involved in mitochondrial RNA editing.It is involved in editing of the mitochondrial tatC transcript at site 581
Protein Family:	PPR
Subclass:	E
Construct Structure:	PLS-E
Gene ID & Species:	AT2G46050 (Arabidopsis thaliana)
Edited Gene(s):	tatC mttB
Editing Type(s):	C-to-U (5)
Publication(s):	[1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170] [2] The Pentatricopeptide Repeat Protein MEF31 is Required for Editing at Site 581 of the Mitochondrial tatC Transcript and Indirectly Influences Editing at Site 586 of the Same Transcript., Plant & cell physiology, 2018. [PMID=29216369]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT2G46050

Mitochondrion

581

CDS

C-to-U

NA=>NA

NA=>NA

NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	74.08%	High	None	32098170

Arabidopsis thaliana

AT2G46050

Mitochondrion

581

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	Control	Normal	Leaf	4 weeks	Direct sequence analysis of RT-PCR products	100.00%	Complete	None	29216369
SAIL_451_B04;CS820747	mef31-1	T-DNA insertion	The insertion in the mutant is located in the sixth PPR motif	Homozygous	No phenotypic alterations were observed during growth of mef31 mutants when compared with wild-type plants, at least under standard growth conditions	Leaf	4 weeks	Direct sequence analysis of RT-PCR products	0.00%	Unedited	Absent	29216369
SALK_146433	mef31-2	T-DNA insertion	The insertion in the mutant is located in the ninth PPR motif	Homozygous	No phenotypic alterations were observed during growth of mef32 mutants when compared with wild-type plants, at least under standard growth conditions	Leaf	4 weeks	Direct sequence analysis of RT-PCR products	0.00%	Unedited	Absent	29216369
SAIL_451_B04;CS820747	mef31-1/35S::MEF31	T-DNA insertion;Complemented	The insertion in the mutant is located in the sixth PPR motif;mef31-1 plants transformed with the wild-type MEF31 gene	Homozygous	No phenotypic alterations were observed during growth of mef33 mutants when compared with wild-type plants, at least under standard growth conditions	Leaf	4 weeks	Direct sequence analysis of RT-PCR products	80.00%	High	Restored	29216369

Last update: Jul 2021 (version 1.0)