MEF31 - Plant Editosome Database

Editing Factor:	MEF31
Synonym:	MITOCHONDRIAL EDITING FACTOR 31
Description:	E-PPR protein involved in mitochondrial RNA editing.It is involved in editing of the mitochondrial tatC transcript at site 581
Protein Family:	PPR
Subclass:	E
Physical Interaction:	NA
Construct Structure:	PLS-E
Gene ID & Species:	AT2G46050 (Arabidopsis thaliana)
Edited Gene(s):	tatC mttB
Editing Type(s):	C-to-U (5)
Publication(s):	[1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170] [2] The Pentatricopeptide Repeat Protein MEF31 is Required for Editing at Site 581 of the Mitochondrial tatC Transcript and Indirectly Influences Editing at Site 586 of the Same Transcript., Plant & cell physiology, 2018. [PMID=29216369]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT2G46050

Mitochondrion

581

CDS

EnsemblGenomes (release 39)

BK010421

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	74.08%	High	None	32098170

Arabidopsis thaliana

AT2G46050

Mitochondrion

581

CDS

C-to-U

CCA=>CUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	Control	Control	Control	Normal	Leaf	4 weeks	Direct sequence analysis of RT-PCR products	100.00%	Complete	None	29216369
SAIL_451_B04;CS820747	mef31-1	T-DNA insertion	The insertion in the mutant is located in the sixth PPR motif	Homozygous	No phenotypic alterations were observed during growth of mef31 mutants when compared with wild-type plants, at least under standard growth conditions	Leaf	4 weeks	Direct sequence analysis of RT-PCR products	0.00%	Unedited	Absent	29216369
SALK_146433	mef31-2	T-DNA insertion	The insertion in the mutant is located in the ninth PPR motif	Homozygous	No phenotypic alterations were observed during growth of mef32 mutants when compared with wild-type plants, at least under standard growth conditions	Leaf	4 weeks	Direct sequence analysis of RT-PCR products	0.00%	Unedited	Absent	29216369
SAIL_451_B04;CS820747	mef31-1/35S::MEF31	T-DNA insertion;Complemented	The insertion in the mutant is located in the sixth PPR motif;mef31-1 plants transformed with the wild-type MEF31 gene	Homozygous	No phenotypic alterations were observed during growth of mef33 mutants when compared with wild-type plants, at least under standard growth conditions	Leaf	4 weeks	Direct sequence analysis of RT-PCR products	80.00%	High	Restored	29216369