Plant Editosome Database
a curated database of RNA editosome in plants

MEF47 - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: MEF47
Synonym: NA
Description: pentatricopeptide (PPR) repeat-containing protein;(source:Araport11)
Protein Family: PPR
Subclass: E
Construct Structure: PLS-E1-E2
Gene ID & Species: At3g16610 (Arabidopsis thaliana)
Edited Gene(s): nad3    ccmC
Editing Type(s): C-to-U (6)
Publication(s): [1] MEF46 and MEF47 are novel specificity factors for RNA editing sites in mitochondrial nad transcripts, Mitochondrion, 2020. [PMID=32439621]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana At3g16610 Mitochondrion ccmC 614 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColumbiaCol-0No treatmentNo treatmentNo treatmentNormalLeaf3-week-old plantletsDirect cDNA sequencing and RT-PCR. RNA editing levels were e100.00%CompleteNone32439621
SALK_059706 (L)mef47-1A T-DNA InsertionLocation of the T-DNA insertions in the mutant and its respective left borders(LB) is at the fourth P motif from ATG.HomozygousThe mutant line of MEF47 displayed a slightly slower growth phenotype.Leaf3-week-old plantletsDirect cDNA sequencing and RT-PCR. RNA editing levels were e0.00%UneditedAbsent32439621
SALK_059706 (L)mef47-1+35S:MEF47ComplementedLocation of the T-DNA insertions in the mutant and its respective left borders(LB) is at the second S motif from ATG. Overexpression of the MEF47 locus under the 35S promoter in the mef47-1 mutant linHomozygousGrowth rate at the wild type levelLeaf3-week-old plantletsDirect cDNA sequencing and RT-PCR. RNA editing levels were e100.00%CompleteRestored32439621
Arabidopsis thaliana At3g16610 Mitochondrion nad3 64 CDS C-to-U NA=>NA NA=>NA NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
ColumbiaCol-0No treatmentNo treatmentNo treatmentNormalLeaf3-week-old plantletsDirect cDNA sequencing and RT-PCR. RNA editing levels were e71.43%HighNone32439621
SALK_059706 (L)mef47-1A T-DNA InsertionLocation of the T-DNA insertions in the mutant and its respective left borders(LB) is at the fourth P motif from ATG.HomozygousThe mutant line of MEF47 displayed a slightly slower growth phenotype.Leaf3-week-old plantletsDirect cDNA sequencing and RT-PCR. RNA editing levels were e0.00%UneditedAbsent32439621
SALK_059706 (L)mef47-1+35S:MEF47ComplementedLocation of the T-DNA insertions in the mutant and its respective left borders(LB) is at the second S motif from ATG. Overexpression of the MEF47 locus under the 35S promoter in the mef47-1 mutant linHomozygousGrowth rate at the wild type levelLeaf3-week-old plantletsDirect cDNA sequencing and RT-PCR. RNA editing levels were e76.92%HighRestored32439621
Last update: Jul 2021 (version 1.0)