MEF47 - Plant Editosome Database

Editing Factor:	MEF47
Synonym:	MITOCHONDRIAL EDITING FACTOR 47
Description:	The defective editing site in mef46, nad5-1958, overlaps with the affected sites in dyw2 mutants, while that in mef47, nad3-64 and ccmC-614 do not, further supporting the specific functional connection between E+ subclass PPR proteins and DYW2
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	E
Physical Interaction:	NA
Construct Structure:	PLS-E1-E2
Gene ID & Species:	AT3G16610 (Arabidopsis thaliana)
Edited Gene(s):	nad3 ccmC
Editing Type(s):	C-to-U (6)
Publication(s):	[1] MEF46 and MEF47 are novel specificity factors for RNA editing sites in mitochondrial nad transcripts, Mitochondrion, 2020. [doi:10.1016/j.mito.2020.05.002]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT3G16610

Mitochondrion

614

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	Col-0	Control	Control	No mutant	Normal	Leaf	3-week-old plantlets	Direct cDNA sequencing and RT-PCR	100.00%	Complete	None	32439621
SALK_059706 (L)	mef47-1	A T-DNA Insertion	Location of the T-DNA insertions in the mutant and its respective left borders(LB) is at the fourth P motif from ATG.	Homozygous	The mutant line of MEF47 displayed a slightly slower growth phenotype.	Leaf	3-week-old plantlets	Direct cDNA sequencing and RT-PCR	0.00%	Unedited	Absent	32439621
SALK_059706 (L)	mef47-1+35S:MEF47	Complemented	Location of the T-DNA insertions in the mutant and its respective left borders(LB) is at the second S motif from ATG. Overexpression of the MEF46 locus under the 35S promoter in the mef46-1 mutant lin	Homozygous	Growth rate at the wild type level	Leaf	3-week-old plantlets	Direct cDNA sequencing and RT-PCR	100.00%	Complete	Restored	32439621

Arabidopsis thaliana

AT3G16610

Mitochondrion

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Columbia	Col-0	Control	Control	No mutant	Normal	Leaf	3-week-old plantlets	Direct cDNA sequencing and RT-PCR	71.43%	High	None	32439621
SALK_059706 (L)	mef47-1	A T-DNA Insertion	Location of the T-DNA insertions in the mutant and its respective left borders(LB) is at the fourth P motif from ATG.	Homozygous	The mutant line of MEF47 displayed a slightly slower growth phenotype.	Leaf	3-week-old plantlets	Direct cDNA sequencing and RT-PCR	0.00%	Unedited	Absent	32439621
SALK_059706 (L)	mef47-1+35S:MEF47	Complemented	Location of the T-DNA insertions in the mutant and its respective left borders(LB) is at the second S motif from ATG. Overexpression of the MEF46 locus under the 35S promoter in the mef46-1 mutant lin	Homozygous	Growth rate at the wild type level	Leaf	3-week-old plantlets	Direct cDNA sequencing and RT-PCR	76.92%	High	Restored	32439621