MEF9 - Plant Editosome Database - BIG Data Center

MEF9 - Plant Editosome Database - BIG Data Center

Summary

Editing Factor:	MEF9
Synonym:	MITOCHONDRIAL EDITING FACTOR 9
Description:	Encoding an E-subclass pentatricopeptide repeat protein required for an RNA editing event in the nad7 transcript in mitochondria of Arabidopsis
Protein Family:	PPR
Subclass:	E
Construct Structure:	PLS-E
Gene ID & Species:	AT1G62260 (Arabidopsis thaliana)
Edited Gene(s):	nad7
Editing Type(s):	C-to-U (10)
Publication(s):	[1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170] [2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003] [3] MEF9, an E-subclass pentatricopeptide repeat protein, is required for an RNA editing event in the nad7 transcript in mitochondria of Arabidopsis., Plant physiology, 2010. [PMID=20018598]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT1G62260

Mitochondrion

200

CDS

C-to-U

UCU=>UUU
NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	mef9-1	EMS Mutant	The EMS mutant alters a Trp codon to a termination signal (W346Stop) in the center of the open reading frame	NA	Normal growth phenotype under standard growth chamber conditions comparable to the wild-type plant	Leaf	NA	cDNA sequence analysis	0.00%	Unedited	Absent	20018598
Col-0	mef9-1	Complementation	The wild-type MEF9 gene is stably transformed into the mef9-1 mutant plants	NA	NA	Leaf	NA	cDNA sequence analysis	100.00%	Complete	Restored	20018598
Col-0	mef9-1	Complementation	Col wild-type MEF9 gene is transfected into the mef9-1 mutant protoplasts	NA	NA	Protoplast	NA	cDNA sequence analysis	50.00%	Medium	Decreased	20018598
Col-0	mef9-2	EMS Mutant	The EMS mutant causes a single nucleotide alteration (W581stop) in a location further downstream at the beginning of the E domain	NA	Plant mef9-2 displays a somewhat slower growth with bolting and flower set delayed by about 2 weeks	Leaf	NA	cDNA sequence analysis	0.00%	Unedited	Absent	20018598
Col-0	mef9-2	Complementation	Col Wild-Type Mef9 Gene Was Transfected Into The Mef9-2 Mutant Protoplasts	NA	NA	Protoplast	NA	cDNA sequence analysis	50.00%	Medium	Decreased	20018598
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	cDNA sequence analysis	100.00%	Complete	None	20018598
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	95.60%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003

Last update: Jul 2021 (version 1.0)