MEF9 - Plant Editosome Database

Summary

Editing Factor:	MEF9
Synonym:	MITOCHONDRIAL EDITING FACTOR 9
Description:	Encoding an E-subclass pentatricopeptide repeat protein required for an RNA editing event in the nad7 transcript in mitochondria of Arabidopsis
Protein Family:	PPR
Subclass:	E
Physical Interaction:	NA
Construct Structure:	PLS-E
Gene ID & Species:	AT1G62260 (Arabidopsis thaliana)
Edited Gene(s):	nad7
Editing Type(s):	C-to-U (10)
Publication(s):	[1] The Analysis of the Editing Defects in the Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins., Plants (Basel, Switzerland), 2020. [PMID=32098170] [2] Multiple PPR protein interactions are involved in the RNA editing system in mitochondria and plastids., Proceedings of the National Academy of Sciences of the United States of America, 2017. [PMID=28761003] [3] MEF9, an E-subclass pentatricopeptide repeat protein, is required for an RNA editing event in the nad7 transcript in mitochondria of Arabidopsis., Plant physiology, 2010. [PMID=20018598]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Position Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT1G62260

Mitochondrion

nad7

200

CDS

NA
EnsemblGenomes (release 39)

NA
BK010421

C-to-U

UCU=>UUU
NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	mef9-1	EMS Mutant	The EMS mutant alters a Trp codon to a termination signal (W346Stop) in the center of the open reading frame	NA	Normal growth phenotype under standard growth chamber conditions comparable to the wild-type plant	Leaf	NA	cDNA sequence analysis	0.00%	Unedited	Absent	20018598
Col-0	mef9-1	Complementation	The wild-type MEF9 gene is stably transformed into the mef9-1 mutant plants	NA	NA	Leaf	NA	cDNA sequence analysis	100.00%	Complete	Restored	20018598
Col-0	mef9-1	Complementation	Col wild-type MEF9 gene is transfected into the mef9-1 mutant protoplasts	NA	NA	Protoplast	NA	cDNA sequence analysis	50.00%	Medium	Decreased	20018598
Col-0	mef9-2	EMS Mutant	The EMS mutant causes a single nucleotide alteration (W581stop) in a location further downstream at the beginning of the E domain	NA	Plant mef9-2 displays a somewhat slower growth with bolting and flower set delayed by about 2 weeks	Leaf	NA	cDNA sequence analysis	0.00%	Unedited	Absent	20018598
Col-0	mef9-2	Complementation	Col Wild-Type Mef9 Gene Was Transfected Into The Mef9-2 Mutant Protoplasts	NA	NA	Protoplast	NA	cDNA sequence analysis	50.00%	Medium	Decreased	20018598
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	cDNA sequence analysis	100.00%	Complete	None	20018598
Col-0	WT	Control	Control	Control	NA	NA	NA	RNA-seq	95.60%	High	None	32098170
Col-0	WT	Control	Control	Control	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003