MPR25 - Plant Editosome Database

Editing Factor:	MPR25
Synonym:	MITOCHONDRIAL PPR25
Description:	Encodes a pentatricopeptide repeat protein essential for RNA editing of nad5 transcripts in mitochondria.
Protein Family:	PPR
Subclass:	E,E+
Construct Structure:	PLS-E, PLS-E-E+, NA
Gene ID & Species:	NA (Arabidopsis thaliana) Os04g51350 (Oryza sativa) LOC_OS04G51350 (Oryza sativa)
Edited Gene(s):	nad5
Editing Type(s):	C-to-U (9)
Publication(s):	[1] The dual-targeted RNA editing factor AEF1 is universally conserved among angiosperms and reveals only minor adaptations upon loss of its chloroplast or its mitochondrial target, Plant Mol Biol, 2019. [PMID=31797248] [2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003] [3] Rice MPR25 encodes a pentatricopeptide repeat protein and is essential for RNA editing of nad5 transcripts in mitochondria, The Plant Journal, 2012. [PMID=22747551]

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

Mitochondrion

1580

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Col-0	WT	No treatment	No treatment	No treatment	Normal	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	76.00%	High	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	60.00%	High	Decreased	28761003

Oryza sativa

LOC_OS04G51350

Mitochondrion

1580

CDS

C-to-U

UCA=>UUA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NC0057, O. sativa L. cv. Nipponbare	mpr25	Tos17 insertion	Tos17 is inserted at nucleotide 1327 from the initiation codon in the ninth PPR motif of MPR25	Homozygous	Growth retardation and pale-green leaves	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	22747551
NC0057, O. sativa L. cv. Nipponbare	mpr25	Tos17 insertion	Tos17 is inserted at nucleotide 1327 from the initiation codon in the ninth PPR motif of MPR25	Homozygous	Growth retardation and pale-green leaves	Leaf	4 weeks	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	22747551
NC0057, O. sativa L. cv. Nipponbare	WT	No treatment	No treatment	No treatment	No treatment	Leaf	4 weeks	Direct Sequencing of PCR Products	100.00%	Complete	None	22747551
NC0057, O. sativa L. cv. Nipponbare	WT	No treatment	No treatment	No treatment	No treatment	Leaf	4 weeks	Direct Sequencing of PCR Products	100.00%	Complete	None	22747551
NC0057, O. sativa L. cv. Nipponbare	Complemented	Complementation	NA	NA	NA	Leaf	4 weeks	Direct Sequencing of PCR Products	100.00%	Complete	Restored	22747551

Oryza sativa

Os04g51350

Mitochondrion

1580

CDS

C-to-U

NA=>NA

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	31797248