OTP82 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Aethionema cordifolium

JF794522

Chloroplast

ndhB

836

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Aethionema cordifolium

JF794522

Chloroplast

ndhG

CDS

C-to-U

NA=>NA

S=>F/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	90.00%	High	NA	22583633

Aethionema grandiflorum

Chloroplast

ndhB

836

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Aethionema grandiflorum

Chloroplast

ndhG

CDS

C-to-U

NA=>NA

S=>F/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	60.00%	High	NA	22583633

Arabidopsis thaliana

AT1G08070

Chloroplast
Plastid

ndhB

null

CDS
NA

C-to-U

NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_851_G04	NA	T-DNA insertion	The T-DNA insertion is in CDS	Homozygous	NA	NA	NA	RT-PCR and poisoned primer extension of RT-PCR products is s	NA	NA	None	19934379
Col-0	Col	No treatment	No treatment	No treatment	NA	NA	3 weeks	Poisoned primer extension (PPE) analysis	72.00%	High	None	25649437
SALK_027812	OTP82wt	Complemented	otp82 plants transformed with a construct that includes a wild-type copy of OTP82 driven by the same promoter as used to drive expression of the S3[TD] variant.	NA	NA	NA	3 weeks	Poisoned primer extension (PPE) analysis	88.83%	High	Restored	25649437
SALK_027812	otp82	A T-DNA insertion	A T-DNA insertion (SALK_027812) in At1g08070	NA	NA	NA	3 weeks	Poisoned primer extension (PPE) analysis	1.50%	Poor	Decreased	25649437
SALK_027812	S3[TD]	A T-DNA insertion	A T-DNA insertion (SALK_027812) in At1g08070	NA	NA	NA	3 weeks	Poisoned primer extension (PPE) analysis	51.00%	Medium	Decreased	25649437

Arabidopsis thaliana

AT1G08070

Chloroplast
Plastid

ndhB

836

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Nössen	otp82-1	Ds Insertion	Ds is inserted at site +465 bp	Homozygous	No obvious visible phenotypes	Leaf	15 days	Poisoned Primer Extension (PPE) Assay	0.80%	Poor	Decreased	19845878
Nössen	otp82-1+OTP82	Complementation	Introduction of the wild-type genomic sequence of OTP82	NA	NA	Leaf	15 days	Direct Sequencing of PCR Products	NA	High	Restored	19845878
Nössen	otp82-1+OTP82ΔDYW	Complementation	A truncated version of OTP82 lacking its DYW motif was introduced into otp82-1	NA	NA	Leaf	15 days	Direct Sequencing of PCR Products	NA	High	Restored	19845878
Nössen	otp82-2	Ds Insertion	Ds is inserted site +793 bp	Homozygous	No obvious visible phenotypes	Leaf	15 days	Poisoned Primer Extension (PPE) Assay	0.10%	Poor	Absent	19845878
SALK_027812	otp82-3	T-DNA Insertion	NA	Homozygous	No obvious visible phenotypes	Leaf	15 days	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19845878
SAIL_851_G04	otp82-4	T-DNA Insertion	NA	Homozygous	No obvious visible phenotypes	Leaf	15 days	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19845878
Nössen	WT	No treatment	No treatment	No treatment	No treatment	Leaf	15 days	Poisoned Primer Extension (PPE) Assay	71.10%	High	None	19845878
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	15 days	Poisoned Primer Extension (PPE) Assay	86.30%	High	None	19845878
NA	NA	Gel mobility shift (GMS) assays	NA	NA	NA	NA	NA	NA	NA	NA	NA	22362750
Col-0	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633
Col-0	WT	No treatment	No treatment	No treatment	NA	NA	NA	RNA-seq	87.20%	High	None	32098170
Col-0	WT	No treatment	No treatment	No treatment	Normal	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	96.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	97.00%	High	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	Similar	28761003

Arabidopsis thaliana

AT1G08070

Chloroplast
Plastid

ndhG

null

CDS
NA

C-to-U

NA=>NA

S=>F
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SAIL_851_G04	NA	T-DNA insertion	The T-DNA insertion is in CDS	Homozygous	NA	NA	NA	RT-PCR and poisoned primer extension of RT-PCR products is s	NA	NA	None	19934379
Col-0	Col	No treatment	No treatment	No treatment	NA	NA	3 weeks	Poisoned primer extension (PPE) analysis	69.00%	High	None	25649437
SALK_027812	OTP82wt	Complemented	otp82 plants transformed with a construct that includes a wild-type copy of OTP82 driven by the same promoter as used to drive expression of the S3[TD] variant.	NA	NA	NA	3 weeks	Poisoned primer extension (PPE) analysis	79.67%	High	Restored	25649437
SALK_027812	otp82	A T-DNA insertion	A T-DNA insertion (SALK_027812) in At1g08070	NA	NA	NA	3 weeks	Poisoned primer extension (PPE) analysis	0.00%	Unedited	Absent	25649437
SALK_027812	S3[TD]	A T-DNA insertion	A T-DNA insertion (SALK_027812) in At1g08070	NA	NA	NA	3 weeks	Poisoned primer extension (PPE) analysis	73.83%	Medium	Similar	25649437

Arabidopsis thaliana

AT1G08070

Chloroplast
Plastid

ndhG

CDS

C-to-U

UCC=>UUC
NA=>NA

S=>F
S=>F/L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Nössen	otp82-1	Ds Insertion	Ds is inserted at site +465 bp	Homozygous	No obvious visible phenotypes	Leaf	15 days	Poisoned Primer Extension (PPE) Assay	2.40%	Poor	Decreased	19845878
Nössen	otp82-1+OTP82	Complementation	Introduction of the wild-type genomic sequence of OTP82	NA	NA	Leaf	15 days	Direct Sequencing of PCR Products	NA	High	Restored	19845878
Nössen	otp82-1+OTP82ΔDYW	Complementation	A truncated version of OTP82 lacking its DYW motif was introduced into otp82-1	NA	NA	Leaf	15 days	Direct Sequencing of PCR Products	NA	High	Restored	19845878
Nössen	otp82-2	Ds Insertion	Ds is inserted at site +793 bp	Homozygous	No obvious visible phenotypes	Leaf	15 days	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19845878
SALK_027812	otp82-3	T-DNA Insertion	NA	Homozygous	No obvious visible phenotypes	Leaf	15 days	Poisoned Primer Extension (PPE) Assay	0.00%	Unedited	Absent	19845878
SAIL_851_G04	otp82-4	T-DNA Insertion	NA	Homozygous	No obvious visible phenotypes	Leaf	15 days	Poisoned Primer Extension (PPE) Assay	0.10%	Poor	Absent	19845878
Nössen	WT	No treatment	No treatment	No treatment	No treatment	Leaf	15 days	Poisoned Primer Extension (PPE) Assay	67.20%	High	None	19845878
Col-0	WT	No treatment	No treatment	No treatment	No treatment	Leaf	15 days	Poisoned Primer Extension (PPE) Assay	78.90%	High	None	19845878
NA	NA	Gel mobility shift (GMS) assays	NA	NA	NA	NA	NA	NA	NA	NA	NA	22362750
Col-0	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	90.00%	High	NA	22583633
Col-0	WT	No treatment	No treatment	No treatment	NA	NA	NA	RNA-seq	77.56%	High	None	32098170
Col-0	WT	No treatment	No treatment	No treatment	Normal	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	64.00%	High	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	56.00%	Medium	Similar	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Rosette leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	81.00%	High	Increased	28761003

Arabis hirsuta

Chloroplast

ndhB

836

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SJO02300	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	0.00%	Unedited	NA	22583633

Arabis hirsuta

Chloroplast

ndhG

CDS

C-to-U

NA=>NA

S=>F/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SJO02300	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	<10.00%	Poor	NA	22583633

Barbarea verna

JF794523

Chloroplast

ndhB

836

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Barbarea verna

JF794523

Chloroplast

ndhG

CDS

C-to-U

NA=>NA

S=>F/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	90.00%	High	NA	22583633

Brassica oleracea

JF794524

Chloroplast

ndhB

836

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	0.00%	Unedited	NA	22583633

Brassica oleracea

JF794524

Chloroplast

ndhG

CDS

C-to-U

NA=>NA

S=>F/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	90.00%	High	NA	22583633

Brassica rapa

Chloroplast

ndhB

836

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	0.00%	Unedited	NA	22583633

Brassica rapa

Chloroplast

ndhG

CDS

C-to-U

NA=>NA

S=>F/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	90.00%	High	NA	22583633

Capsella bursa-pastoris

JF794525

Chloroplast

ndhB

836

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Serial # 0051714	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Capsella bursa-pastoris

JF794525

Chloroplast

ndhG

CDS

C-to-U

NA=>NA

S=>F/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Serial # 0051714	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	80.00%	High	NA	22583633

Crucihimalaya wallichii

JF794526

Chloroplast

ndhB

836

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SJS00500	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Crucihimalaya wallichii

JF794526

Chloroplast

ndhG

CDS

C-to-U

NA=>NA

S=>F/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SJS00500	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	90.00%	High	NA	22583633

Draba nemorosa

JF794537

Chloroplast

ndhB

836

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SJO02100	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	0.00%	Unedited	NA	22583633

Draba nemorosa

JF794537

Chloroplast

ndhG

CDS

C-to-U

NA=>NA

S=>F/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SJO02100	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	0.00%	Unedited	NA	22583633

Hesperis matronalis

JF794527

Chloroplast

ndhB

836

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
PI 586611	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	0.00%	Unedited	NA	22583633

Hesperis matronalis

JF794527

Chloroplast

ndhG

CDS

C-to-U

NA=>NA

S=>F/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
PI 586611	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	90.00%	High	NA	22583633

Iberis amara

JF794529

Chloroplast

ndhB

836

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
PI 633243	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	0.00%	Unedited	NA	22583633

Iberis amara

JF794529

Chloroplast

ndhG

CDS

C-to-U

NA=>NA

S=>F/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
PI 633243	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	20.00%	Low	NA	22583633

Isatis tinctoria

JF794528

Chloroplast

ndhB

836

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	0.00%	Unedited	NA	22583633

Isatis tinctoria

JF794528

Chloroplast

ndhG

CDS

C-to-U

NA=>NA

S=>F/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	90.00%	High	NA	22583633

Lepidium sativum

JF794530

Chloroplast

ndhB

836

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Lepidium sativum

JF794530

Chloroplast

ndhG

CDS

C-to-U

NA=>NA

S=>F/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	90.00%	High	NA	22583633

Lepidium virginicum

JF794531

Chloroplast

ndhB

836

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SJO02600	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Lepidium virginicum

JF794531

Chloroplast

ndhG

CDS

C-to-U

NA=>NA

S=>F/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SJO02600	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	90.00%	High	NA	22583633

Lobularia maritima

Chloroplast

ndhB

836

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	0.00%	Unedited	NA	22583633

Lobularia maritima

Chloroplast

ndhG

CDS

C-to-U

NA=>NA

S=>F/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	<10.00%	Poor	NA	22583633

Matthiola incana

JF794532

Chloroplast

ndhB

836

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Matthiola incana

JF794532

Chloroplast

ndhG

CDS

C-to-U

NA=>NA

S=>F/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	70.00%	High	NA	22583633

Nasturtium officinale

JF794533

Chloroplast

ndhB

836

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Serial #0200327	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	90.00%	High	NA	22583633

Nasturtium officinale

JF794533

Chloroplast

ndhG

CDS

C-to-U

NA=>NA

S=>F/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Serial #0200327	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	90.00%	High	NA	22583633

Olimarabidopsis pumila

JF794534

Chloroplast

ndhB

836

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SJS00200	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	100.00%	Complete	NA	22583633

Olimarabidopsis pumila

JF794534

Chloroplast

ndhG

CDS

C-to-U

NA=>NA

S=>F/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SJS00200	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	80.00%	High	NA	22583633

Raphanus sativus

JF794535

Chloroplast

ndhB

836

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	0.00%	Unedited	NA	22583633

Raphanus sativus

JF794535

Chloroplast

ndhG

CDS

C-to-U

NA=>NA

S=>F/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	90.00%	High	NA	22583633

Thlaspi arvense

JF794536

Chloroplast

ndhB

836

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Ames 29531	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	0.00%	Unedited	NA	22583633

Thlaspi arvense

JF794536

Chloroplast

ndhG

CDS

C-to-U

NA=>NA

S=>F/L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
Ames 29531	NA	NA	NA	NA	NA	Leaf	NA	PCR amplification and bulk sequencing	90.00%	High	NA	22583633

Editing Factor:	OTP82
Synonym:	EMB3102, EMBRYO DEFECTIVE 3102, ORGANELLE TRANSCRIPT PROCESSING 82
Description:	Encodes a factor required for RNA editing of plastid ndhB and ndhG transcripts.
Protein Family:	PPR
Subclass:	DYW
Construct Structure:	PLS-E-DYW, PLS-E-E+-DYW, NA
Gene ID & Species:	JF794522 (Aethionema cordifolium) NA (Aethionema grandiflorum) AT1G08070 (Arabidopsis thaliana) NA (Arabis hirsuta) JF794523 (Barbarea verna) JF794524 (Brassica oleracea) NA (Brassica rapa) pastoris (Capsella bursa) JF794526 (Crucihimalaya wallichii) JF794537 (Draba nemorosa) JF794527 (Hesperis matronalis) JF794529 (Iberis amara) JF794528 (Isatis tinctoria) JF794530 (Lepidium sativum) JF794531 (Lepidium virginicum) NA (Lobularia maritima) JF794532 (Matthiola incana) JF794533 (Nasturtium officinale) JF794534 (Olimarabidopsis pumila) JF794535 (Raphanus sativus) JF794536 (Thlaspi arvense)
Edited Gene(s):	ndhB ndhG
Editing Type(s):	C-to-U (78)
Publication(s):	[1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170] [2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003] [3] Predictable alteration of sequence recognition by RNA editing factors from Arabidopsis, Plant Cell, 2015. [PMID=25649437] [4] Molecular evolution of pentatricopeptide repeat genes reveals truncation in species lacking an editing target and structural domains under distinct selective pressures, BMC Evol Biol, 2012. [PMID=22583633] [5] A pentatricopeptide repeat protein acts as a site-specificity factor at multiple RNA editing sites with unrelated cis-acting elements in plastids, Nucleic Acids Research, 2012. [PMID=22362750] [6] A study of new Arabidopsis chloroplast RNA editing mutants reveals general features of editing factors and their target sites, Plant Cell, 2009. [PMID=19934379] [7] The pentatricopeptide repeat protein OTP82 is required for RNA editing of plastid ndhB and ndhG transcripts, The Plant Journal, 2010. [PMID=19845878]

Plant Editosome Database
a curated database of RNA editosome in plants

Summary

Editing Details