Plant Editosome Database
a curated database of RNA editosome in plants

PPME - Plant Editosome Database - BIG Data Center
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Summary

Editing Factor: PPME
Synonym: P-type PPR-Modulating Editing
Description: Encodes a P-type PPR protein which has a distinct role in mitochondrial nad1 RNA editing via RNA binding activity.
Protein Family: PPR
Subclass: P
Construct Structure: P
Gene ID & Species: AT3G18020 (Arabidopsis thaliana)
Edited Gene(s): nad1
Editing Type(s): C-to-U (16)
Publication(s): [1] The Analysis of the Editing Defects in the dyw2 Mutant Provides New Clues for the Prediction of RNA Targets of Arabidopsis E+-Class PPR Proteins, Plants (Basel), 2020. [PMID=32098170]
[2] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [PMID=28761003]
[3] Distinct role of Arabidopsis mitochondrial P-type pentatricopeptide repeat protein-modulating editing protein, PPME, in nad1 RNA editing, RNA Biology, 2016. [PMID=27149614]

Editing Details

Species Gene ID Organelle Edited Gene Position Region Editing Type Codon Amino Acid Molecular Effect Experiment Details
Arabidopsis thaliana AT3G18020 Mitochondrion nad1 898 CDS C-to-U CGG=>UGG
NA=>NA		 R=>W
NA=>NA		 Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_019722comp1ComplementationCaMV 35S promoter-drivenNANASeedling14 daysDirect Sequencing of PCR ProductsNAMediumRestored27149614
SALK_019722comp2ComplementationPPME native promoterdriven constructsNANASeedling14 daysDirect Sequencing of PCR ProductsNAMediumRestored27149614
SALK_019722ppme-/- seedlingsT-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutantGrowth-retardedSeedling14 daysDirect Sequencing of PCR ProductsNAUneditedAbsent27149614
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentSeedling14 daysDirect Sequencing of PCR ProductsNAMediumNone27149614
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq97.00%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract100.00%CompleteNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract17.00%PoorDecreased28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract3.00%PoorDecreased28761003
Arabidopsis thaliana AT3G18020 Mitochondrion nad1 937 CDS C-to-U CCU=>UCU
NA=>NA		 P=>S
NA=>NA		 Recoding
NA
Experiment Details
Genotype (Ecotype) Allele Treatment Treatment Detail Mutant Type Phenotype Tissue Development Stage Detection Method Editing Frequency Editing Extent Mutant Effect PMID
SALK_019722comp1ComplementationCaMV 35S promoter-drivenNANASeedling14 daysDirect Sequencing of PCR ProductsNAMediumRestored27149614
SALK_019722comp2ComplementationPPME native promoterdriven constructsNANASeedling14 daysDirect Sequencing of PCR ProductsNAMediumRestored27149614
SALK_019722ppme-/- seedlingsT-DNA InsertionT-DNA is inserted in the coding regionHomozygous; Null mutantGrowth-retardedSeedling14 daysDirect Sequencing of PCR ProductsNAUneditedDecreased27149614
Col-0WTNo treatmentNo treatmentNo treatmentNo treatmentSeedling14 daysDirect Sequencing of PCR ProductsNAMediumNone27149614
Col-0WTNo treatmentNo treatmentNo treatmentNANANARNA-seq95.41%HighNone32098170
Col-0WTNo treatmentNo treatmentNo treatmentNormalRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract99.00%HighNone28761003
Col-0DYW2-GFP-OE1Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract97.00%HighSimilar28761003
Col-0DYW2-GFP-OE2Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 geneCol-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independenNASlow growthRosette leafNART-PCR and calculated with the DNADynamo software (BlueTract96.00%HighSimilar28761003
Last update: Jul 2021 (version 1.0)