PPME - Plant Editosome Database

Summary

Editing Factor:	PPME
Synonym:	P-type PPR-Modulating Editing
Description:	Modulating Editing (PPME), which has a distinct role in mitochondrial nad1 RNA editing via RNA binding activity; In the homozygous ppme mutant, cytosine (C)-to-uracil (U) conversions at both the nad1-898 and 937 sites were abolished, disrupting Arg(300)-to-Trp(300) and Pro(313)-to-Ser(313) amino acid changes in the mitochondrial NAD1 protein; NAD1 is a critical component of mitochondrial respiration complex I; its activity is severely reduced in the homozygous ppme mutant, resulting in significantly altered growth and development; Both abolished RNA editing and defective complex I activity were completely rescued by CaMV 35S promoter- and PPME native promoter-driven PPME genomic fragments tagged with GFP in a homozygous ppme background
Protein Family:	Pentatricopeptide repeat (PPR) protein
Subclass:	P
Physical Interaction:	NA
Construct Structure:	P
Gene ID & Species:	AT3G18020 (Arabidopsis thaliana)
Edited Gene(s):	nad1
Editing Type(s):	C-to-U (14)
Publication(s):	[1] Multiple PPR Protein Interactions Are Involved in the RNA Editing System in Arabidopsis Mitochondria and Plastids, Proc Natl Acad Sci U S A, 2017. [doi:10.1073/pnas.1705815114] [2] Distinct role of Arabidopsis mitochondrial P-type pentatricopeptide repeat protein-modulating editing protein, PPME, in nad1 RNA editing, RNA Biology, 2016. [doi:10.1080/15476286.2016.1184384]

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Nuclear Genome

Organelle Genome

Other Region

Other Position

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Arabidopsis thaliana

AT3G18020

Mitochondrion

nad1

898

CDS

C-to-U

CGG=>UGG
NA=>NA

R=>W
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
SALK_019722	comp1	Complementation	CaMV 35S promoter-driven	NA	NA	Seedling	14 days	Direct Sequencing of PCR Products	NA	Medium	Restored	27149614
SALK_019722	comp2	Complementation	PPME native promoterdriven constructs	NA	NA	Seedling	14 days	Direct Sequencing of PCR Products	NA	Medium	Restored	27149614
SALK_019722	ppme-/- seedlings	T-DNA Insertion	T-DNA is inserted in the coding region	Homozygous	Growth-retarded	Seedling	14 days	Direct Sequencing of PCR Products	NA	Unedited	Absent	27149614
Col-0	WT	Control	Control	No mutant	Normal	Seedling	14 days	Direct Sequencing of PCR Products	NA	Medium	None	27149614
Col-0	WT	Control	Control	No mutant	Normal	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	100.00%	Complete	None	28761003
Col-0	DYW2-GFP-OE1	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	17.00%	Poor	Decreased	28761003
Col-0	DYW2-GFP-OE2	Generating Arabidopsis plants overexpressing the GFP-tagged DYW2 gene	Col-0 A. thaliana plants were transformed with the 35S::DYW2-GFP plasmids (in pK7WGF2 backbone) by the floral-dip method. Transformed plants were selected based on Kanamycin resistance. Two independen	NA	Slow growth	Leaf	NA	RT-PCR and calculated with the DNADynamo software (BlueTract	3.00%	Poor	Decreased	28761003