Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Gransden | WT Gransden | No treatment | No treatment | No treatment | Normal | NA | NA | cDNA sequencing and performing peak height calculations with | 100.00% | Complete | None | 32616665 |
| Reute | WT Reute | No treatment | No treatment | No treatment | Wild type Reute can produce viable sporophytes (scale bar: 1 mm). | NA | NA | cDNA sequencing and performing peak height calculations with | 100.00% | Complete | None | 32616665 |
| Gransden | ko Gransden ppr79 | Knockout | We generated knockout lines in P. patens PPR79 by homologous recombination after polyethylene glycol (PEG)-mediated transformation (Hohe et al., 2004) of P. patens (Hedw.) Bruch & Schimp. strain Grans | Knockout | Gametophores from ppr79 Gransden ko lines (Schallenberg-Rüdinger et al., 2013) grew more slowly compared to the wild type and showed a dwarf phenotype with small, spikier leafy structures. | NA | NA | cDNA sequencing and performing peak height calculations with | 0.00% | Unedited | Absent | 32616665 |
| Reute | ko Reute ppr79 | Knockout | We generated knockout lines in P. patens PPR79 by homologous recombination after polyethylene glycol (PEG)-mediated transformation (Hohe et al., 2004) of P. patens (Hedw.) Bruch & Schimp. strain Grans | Knockout | ko Reute ppr79 can produce viable sporophytes (scale bar: 1 mm). The same growth phenotype as ppr79 Gransden ko plants. The few mature ppr79 Reute ko sporophytes that developed were nonetheless able to produce fertile spores, which germinated and yielded new gametophores when spread on solid growth medium. Hence, although less efficiently than the wild type, ppr79 Reute ko plants can complete their entire sexual life cycle. | NA | NA | cDNA sequencing and performing peak height calculations with | 0.00% | Unedited | Absent | 32616665 |
| NA | P. patens PPR79 | Transformation and Complementation | Physcomitrium patens PPR79 was introduced into the ppr79 ko line. | Knockout | NA | NA | NA | cDNA sequencing and performing peak height calculations with | 100.00% | Complete | Restored | 32616665 |
| NA | F. hygrometrica PPR79 | Transformation and Complementation | The Funaria hygrometrica putative PPR79 orthologue was introduced into the ppr79 ko line. | Knockout | NA | NA | NA | cDNA sequencing and performing peak height calculations with | 100.00% | Complete | Restored | 32616665 |
| NA | A. thaliana CWM1 | Transformation and Complementation | Arabidopsis CWM1 was introduced into the ppr79 ko line. | Knockout | NA | NA | NA | cDNA sequencing and performing peak height calculations with | 0.00% | Unedited | Absent | 32616665 |
| NA | CWM1-DYWPPR79 | Transformation and Complementation | Chimeric constructs between P. patens PPR79 and Arabidopsis CWM1 (CWM1-DYWPPR79) was introduced into the ppr79 ko line. | Knockout | NA | NA | NA | cDNA sequencing and performing peak height calculations with | 0.00% | Unedited | Absent | 32616665 |
| NA | CWM1PLS-E1E2DYWPPR79 | Transformation and Complementation | Chimeric constructs between P. patens PPR79 and Arabidopsis CWM1 (CWM1PLS-E1E2DYWPPR79) was introduced into the ppr79 ko line. | Knockout | NA | NA | NA | cDNA sequencing and performing peak height calculations with | 0.00% | Unedited | Absent | 32616665 |
| NA | Pp ppr79 ko complementation Line 1 | Transformation and Complementation | CWM1 targets in Macadamia and transgenic lines in the P. patens ppr79 ko expressing Macadamia CWM1 | Knockout | NA | NA | NA | cDNA sequencing and performing peak height calculations with | 100.00% | High | Restored | 32616665 |
| NA | Pp ppr79 ko complementation Line 2 | Transformation and Complementation | CWM1 targets in Macadamia and transgenic lines in the P. patens ppr79 ko expressing Macadamia CWM1 | Knockout | NA | NA | NA | cDNA sequencing and performing peak height calculations with | 40.00% | Medium | Decreased | 32616665 |