Experiment Details
| Genotype (Ecotype) |
Allele |
Treatment |
Treatment Detail |
Mutant Type |
Phenotype |
Tissue |
Development Stage |
Detection Method |
Editing Frequency |
Editing Extent |
Mutant Effect |
PMID |
| Gransden | WT Gransden | Control | Control | Control | Normal | NA | NA | cDNA sequencing by Macrogen Europe, and peak height calculat | 100.00% | Complete | None | 32616665 |
| Reute | WT Reute | Control | Control | Control | Wild type Reute can produce viable sporophytes (scale bar: 1 mm). | NA | NA | cDNA sequencing by Macrogen Europe, and peak height calculat | 100.00% | Complete | None | 32616665 |
| Gransden | ko Gransden ppr79 | Knockout | This research generated knockout lines in P. patens PPR79 by homologous recombination after polyethylene glycol (PEG)-mediated transformation of P. patens strain Gransden and P. patens ecotype Reute p | Knockout | Gametophores from ppr79 Gransden ko lines (Schallenberg-Rüdinger et al., 2013) grew more slowly compared to the wild type and showed a dwarf phenotype with small, spikier leafy structures. | NA | NA | cDNA sequencing by Macrogen Europe, and peak height calculat | 0.00% | Unedited | Absent | 32616665 |
| Reute | ko Reute ppr79 | Knockout | This research generated knockout lines in P. patens PPR79 by homologous recombination after polyethylene glycol (PEG)-mediated transformation of P. patens strain Gransden and P. patens ecotype Reute p | Knockout | ko Reute ppr79 can produce viable sporophytes (scale bar: 1 mm). The same growth phenotype as ppr79 Gransden ko plants. The few mature ppr79 Reute ko sporophytes that developed were nonetheless able to produce fertile spores, which germinated and yielded new gametophores when spread on solid growth medium. Hence, although less efficiently than the wild type, ppr79 Reute ko plants can complete their entire sexual life cycle. | NA | NA | cDNA sequencing by Macrogen Europe, and peak height calculat | 0.00% | Unedited | Absent | 32616665 |
| NA | P. patens PPR79 | Transformation and Complementation | Physcomitrium patens PPR79 was introduced into the ppr79 ko line. | Knockout | NA | NA | NA | cDNA sequencing by Macrogen Europe, and peak height calculat | 100.00% | Complete | Restored | 32616665 |
| NA | F. hygrometrica PPR79 | Transformation and Complementation | The Funaria hygrometrica putative PPR79 orthologue was introduced into the ppr79 ko line. | Knockout | NA | NA | NA | cDNA sequencing by Macrogen Europe, and peak height calculat | 100.00% | Complete | Restored | 32616665 |
| NA | A. thaliana CWM1 | Transformation and Complementation | Arabidopsis CWM1 was introduced into the ppr79 ko line. | Knockout | NA | NA | NA | cDNA sequencing by Macrogen Europe, and peak height calculat | 0.00% | Unedited | Absent | 32616665 |
| NA | CWM1-DYWPPR79 | Transformation and Complementation | Chimeric constructs between P. patens PPR79 and Arabidopsis CWM1 (CWM1-DYWPPR79) was introduced into the ppr79 ko line. | Knockout | NA | NA | NA | cDNA sequencing by Macrogen Europe, and peak height calculat | 0.00% | Unedited | Absent | 32616665 |
| NA | CWM1PLS-E1E2DYWPPR79 | Transformation and Complementation | Chimeric constructs between P. patens PPR79 and Arabidopsis CWM1 (CWM1PLS-E1E2DYWPPR79) was introduced into the ppr79 ko line. | Knockout | NA | NA | NA | cDNA sequencing by Macrogen Europe, and peak height calculat | 0.00% | Unedited | Absent | 32616665 |
| NA | Pp ppr79 ko complementation Line 1 | Transformation and Complementation | CWM1 targets in Macadamia and transgenic lines in the P. patens ppr79 ko expressing Macadamia CWM1 | Knockout | NA | NA | NA | cDNA sequencing by Macrogen Europe, and peak height calculat | 100.00% | Complete | Restored | 32616665 |
| NA | Pp ppr79 ko complementation Line 2 | Transformation and Complementation | CWM1 targets in Macadamia and transgenic lines in the P. patens ppr79 ko expressing Macadamia CWM1 | Knockout | NA | NA | NA | cDNA sequencing by Macrogen Europe, and peak height calculat | 40.00% | Medium | Decreased | 32616665 |