PPRSp1 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Hordeum vulgare

LOC109783329

Mitochondrion

nad3

147

CDS

C-to-U

UCC=>UCU

S=>S

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Control	Control	Control	Control	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	14.00%	Poor	None	32358688
NA	2H	Salt stressed	2 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	61.75%	High	None	32358688
NA	12H	Salt stressed	12 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	92.75%	High	None	32358688
NA	24H	Salt stressed	24 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	39.58%	Low	None	32358688

Hordeum vulgare

LOC109783329

Mitochondrion

nad3

185

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Control	Control	Control	Control	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	24.95%	Low	None	32358688
NA	2H	Salt stressed	2 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	39.89%	Low	None	32358688
NA	12H	Salt stressed	12 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	39.95%	Low	None	32358688
NA	24H	Salt stressed	24 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	59.62%	Medium	None	32358688

Hordeum vulgare

LOC109783329

Mitochondrion

nad3

190

CDS

C-to-U

CCU=>UCU

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Control	Control	Control	Control	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	10.00%	Poor	None	32358688
NA	2H	Salt stressed	2 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	47.83%	Medium	None	32358688
NA	12H	Salt stressed	12 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	48.91%	Medium	None	32358688
NA	24H	Salt stressed	24 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	11.70%	Poor	None	32358688

Hordeum vulgare

LOC109783329

Mitochondrion

nad3

191

CDS

C-to-U

CCU=>CUU

P=>S

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Control	Control	Control	Control	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	9.98%	Poor	None	32358688
NA	2H	Salt stressed	2 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	48.91%	Medium	None	32358688
NA	12H	Salt stressed	12 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	53.86%	Medium	None	32358688
NA	24H	Salt stressed	24 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	11.70%	Poor	None	32358688

Hordeum vulgare

LOC109783329

Mitochondrion

nad3

208

CDS

C-to-U

CCU=>UUU

P=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Control	Control	Control	Control	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	0.00%	Unedited	None	32358688
NA	2H	Salt stressed	2 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	100.00%	Complete	None	32358688
NA	12H	Salt stressed	12 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	100.00%	Complete	None	32358688
NA	24H	Salt stressed	24 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	15.75%	Poor	None	32358688

Hordeum vulgare

LOC109783329

Mitochondrion

nad3

209

CDS

C-to-U

CCU=>UUU

P=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Control	Control	Control	Control	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	0.00%	Unedited	None	32358688
NA	2H	Salt stressed	2 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	100.00%	Complete	None	32358688
NA	12H	Salt stressed	12 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	100.00%	Complete	None	32358688
NA	24H	Salt stressed	24 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	15.75%	Poor	None	32358688

Hordeum vulgare

LOC109783329

Mitochondrion

nad3

275

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Control	Control	Control	Control	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	0.00%	Unedited	None	32358688
NA	2H	Salt stressed	2 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	69.95%	High	None	32358688
NA	12H	Salt stressed	12 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	74.93%	High	None	32358688
NA	24H	Salt stressed	24 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	13.92%	Poor	None	32358688

Hordeum vulgare

LOC109783329

Mitochondrion

nad3

317

CDS

C-to-U

UCU=>UUU

S=>F

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Control	Control	Control	Control	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	11.85%	Poor	None	32358688
NA	2H	Salt stressed	2 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	74.89%	High	None	32358688
NA	12H	Salt stressed	12 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	100.00%	Complete	None	32358688
NA	24H	Salt stressed	24 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	59.72%	Medium	None	32358688

Hordeum vulgare

LOC109783329

Mitochondrion

nad3

344

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Control	Control	Control	Control	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	6.92%	Poor	None	32358688
NA	2H	Salt stressed	2 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	100.00%	Complete	None	32358688
NA	12H	Salt stressed	12 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	39.91%	Low	None	32358688
NA	24H	Salt stressed	24 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	6.93%	Poor	None	32358688

Hordeum vulgare

LOC109783329

Mitochondrion

nad3

349

CDS

C-to-U

CGG=>UGG

R=>W

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Control	Control	Control	Control	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	0.00%	Unedited	None	32358688
NA	2H	Salt stressed	2 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	100.00%	Complete	None	32358688
NA	12H	Salt stressed	12 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	39.83%	Low	None	32358688
NA	24H	Salt stressed	24 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	0.00%	Unedited	None	32358688

Hordeum vulgare

LOC109783329

Mitochondrion

nad3

CDS

C-to-U

AUC=>AUU

I=>I

Synonymous

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Control	Control	Control	Control	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	0.00%	Unedited	None	32358688
NA	2H	Salt stressed	2 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	85.71%	High	None	32358688
NA	12H	Salt stressed	12 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	85.92%	High	None	32358688
NA	24H	Salt stressed	24 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	2.61%	Poor	None	32358688

Hordeum vulgare

LOC109783329

Mitochondrion

nad3

CDS

C-to-U

CCG=>CUG

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Control	Control	Control	Control	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	10.96%	Poor	None	32358688
NA	2H	Salt stressed	2 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	85.94%	High	None	32358688
NA	12H	Salt stressed	12 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	100.00%	Complete	None	32358688
NA	24H	Salt stressed	24 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	29.87%	Low	None	32358688

Hordeum vulgare

LOC109783329

Mitochondrion

nad3

CDS

C-to-U

UCG=>UUG

S=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Control	Control	Control	Control	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	25.00%	Low	None	32358688
NA	2H	Salt stressed	2 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	48.82%	Medium	None	32358688
NA	12H	Salt stressed	12 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	100.00%	Complete	None	32358688
NA	24H	Salt stressed	24 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	19.73%	Poor	None	32358688

Hordeum vulgare

LOC109783329

Mitochondrion

nad3

CDS

C-to-U

CCA=>UUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Control	Control	Control	Control	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	22.99%	Low	None	32358688
NA	2H	Salt stressed	2 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	85.98%	High	None	32358688
NA	12H	Salt stressed	12 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	100.00%	Complete	None	32358688
NA	24H	Salt stressed	24 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	0.00%	Unedited	None	32358688

Hordeum vulgare

LOC109783329

Mitochondrion

nad3

CDS

C-to-U

CCA=>UUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Control	Control	Control	Control	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	22.99%	Low	None	32358688
NA	2H	Salt stressed	2 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	85.98%	High	None	32358688
NA	12H	Salt stressed	12 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	100.00%	Complete	None	32358688
NA	24H	Salt stressed	24 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	0.00%	Unedited	None	32358688

Hordeum vulgare

LOC109783329

Mitochondrion

nad3

CDS

C-to-U

CCA=>UUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Control	Control	Control	Control	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	24.00%	Low	None	32358688
NA	2H	Salt stressed	2 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	86.91%	High	None	32358688
NA	12H	Salt stressed	12 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	100.00%	Complete	None	32358688
NA	24H	Salt stressed	24 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	20.00%	Low	None	32358688

Hordeum vulgare

LOC109783329

Mitochondrion

nad3

CDS

C-to-U

CCA=>UUA

P=>L

Recoding

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	Control	Control	Control	Control	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	24.00%	Low	None	32358688
NA	2H	Salt stressed	2 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	86.91%	High	None	32358688
NA	12H	Salt stressed	12 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	100.00%	Complete	None	32358688
NA	24H	Salt stressed	24 hours after 50 mM salinity exposure	Non-mutant	NA	Leaf	NA	Detected by CLC genomic workbench 3.6.5, validated by qRT-PC	20.00%	Low	None	32358688

Editing Factor:	PPRSp1
Synonym:	NA
Description:	Position - 6 in cis-element upstream editing sites of C155, C190 and C191 may be vital to the editing process in these sites by PPRSp1 protein; The differential editing of this gene under salinity led to a relationship between RNA editing and cellular respiration regulation
Protein Family:	PPR
Subclass:	DYW
Construct Structure:	PLS-DYW
Gene ID & Species:	LOC109783329 (Hordeum vulgare)
Edited Gene(s):	nad3
Editing Type(s):	C-to-U (68)
Publication(s):	[1] Salinity effects on nad3 gene RNA editing of wild barley mitochondria., Molecular biology reports, 2020. [PMID=32358688]

Plant Editosome Database
a curated database of RNA editosome in plants

Summary

Editing Details