PpPPR_56 - Plant Editosome Database

Editing Details

Species

Gene ID

Organelle

Edited Gene

Position

Region

Editing Type

Codon

Amino Acid

Molecular Effect

Experiment Details

Physcomitrium patens

Mitochondrion

nad3

230

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Wild Type	Wild Type	No mutant	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	83.33%	High	None	29718364
NA	△56-22	Knockout (KO) line	Procedures to obtain moss lines PpPPR_56 KO (Δ56-22) and PpPPR_71 KO (Δ71 6-11) have been previously described (Ohtani et al. 2010, Tasaki et al. 2010).	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56FL	Complementation	Full-length (FL) version of PpPPR_56 was transformed into the respective KO moss background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	86.84%	High	Restored	29718364
NA	56ΔE/DYW	Complementation	Truncated version (ΔE/DYW) of PpPPR_56 was transformed into the respective KO moss background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56△DYW	Complementation	Truncated version (ΔDYW) of PpPPR_56 was transformed into the respective KO moss background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56△E	Complementation	Truncated version (ΔE) of PpPPR_56 was transformed into the respective KO moss background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR+71E/71DYW	Complementation	Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR+71E/56DYW	Complementation	Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR+56E/71DYW	Complementation	Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR+56E/56DYW	Complementation	Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	95.00%	High	Restored	29718364
NA	56PPR+56E/45DYW	Complementation	Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	35.00%	Low	Restored	29718364
NA	56PPR+56E/65DYW	Complementation	Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR+56E/77DYW	Complementation	Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR+56E/78DYW	Complementation	Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR+56E/79DYW	Complementation	Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR+56E/91DYW	Complementation	Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR+56E/98DYW	Complementation	Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56M1	Complementation	To investigate their roles in RNA editing, we generated transgenic lines with mutated DYW domains in which the HxExnCxxC signature was changed to AxAxnCxxC (56M1 and 71M1) and HxExnAxxA (56M2 and 71M2	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56M2	Complementation	To investigate their roles in RNA editing, we generated transgenic lines with mutated DYW domains in which the HxExnCxxC signature was changed to AxAxnCxxC (56M1 and 71M1) and HxExnAxxA (56M2 and 71M2	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56M3	Complementation	To investigate their roles in RNA editing, we generated transgenic lines with mutated DYW domains in which the HxExnCxxC signature was changed to AxAxnCxxC (56M1 and 71M1) and HxExnAxxA (56M2 and 71M2	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR-E-56DYW	Complementation	RNA editing of nad3-C230 and nad4-C272 in various transgenic lines harboring chimeric PpPPR_56 constructs (56PPE-E-r71dywA, -dywB or -dywC) in a PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	90.00%	High	Restored	29718364
NA	56PPR-E-71DYW	Complementation	RNA editing of nad3-C230 and nad4-C272 in various transgenic lines harboring chimeric PpPPR_56 constructs (56PPE-E-r71dywA, -dywB or -dywC) in a PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR-E-r71dywA	Complementation	RNA editing of nad3-C230 and nad4-C272 in various transgenic lines harboring chimeric PpPPR_56 constructs (56PPE-E-r71dywA, -dywB or -dywC) in a PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	70.00%	High	Restored	29718364
NA	56PPR-E-r71dywB	Complementation	RNA editing of nad3-C230 and nad4-C272 in various transgenic lines harboring chimeric PpPPR_56 constructs (56PPE-E-r71dywA, -dywB or -dywC) in a PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	35.00%	Low	Restored	29718364
NA	56PPR-E-r71dywC	Complementation	RNA editing of nad3-C230 and nad4-C272 in various transgenic lines harboring chimeric PpPPR_56 constructs (56PPE-E-r71dywA, -dywB or -dywC) in a PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	85.00%	High	Restored	29718364
NA	56PPR-E-r71dywB-1	Complementation	RNA editing in Δ56 complemented lines with dywB-1 and dywB-2, respectively.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	25.00%	Low	Restored	29718364
NA	56PPR-E-r71dywB-2	Complementation	RNA editing in Δ56 complemented lines with dywB-1 and dywB-2, respectively.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	90.00%	High	Restored	29718364
NA	56PPR-E+71DYW-m1	Complementation	RNA editing efficiency of nad3-C230 and nad4-C272 in Δ56 complemented lines with 56PPR-E + 71DYW-m1 and -m2, respectively.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR-E+71DYW-m2	Complementation	RNA editing efficiency of nad3-C230 and nad4-C272 in Δ56 complemented lines with 56PPR-E + 71DYW-m1 and -m2, respectively.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	15.00%	Poor	Restored	29718364
NA	56PPR-E+78DYW I38M/S39H #4	Complementation	RNA editing in the PpPPR_56 KO mosses complemented with 56PPR-E + 78DYW I38M/S39H.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	15.00%	Poor	Restored	29718364
NA	56PPR-E+78DYW I38M/S39H #6	Complementation	RNA editing in the PpPPR_56 KO mosses complemented with 56PPR-E + 78DYW I38M/S39H.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	20.00%	Low	Restored	29718364
NA	56PPR-E+71DYW A23D	Complementation and mutation	Mutated 56PPR-E/71DYW variants were introduced in the PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR-E+71DYW S25C	Complementation and mutation	Mutated 56PPR-E/71DYW variants were introduced in the PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR-E+71DYW Y26S	Complementation and mutation	Mutated 56PPR-E/71DYW variants were introduced in the PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR-E+71DYW A34V	Complementation and mutation	Mutated 56PPR-E/71DYW variants were introduced in the PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR-E+71DYW V37L/L38M	Complementation and mutation	Mutated 56PPR-E/71DYW variants were introduced in the PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR-E+71DYW S39H	Complementation and mutation	Mutated 56PPR-E/71DYW variants were introduced in the PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR-E+71DYW L40T	Complementation and mutation	Mutated 56PPR-E/71DYW variants were introduced in the PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR-E+71DYW S42P	Complementation and mutation	Mutated 56PPR-E/71DYW variants were introduced in the PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364

Physcomitrium patens

PP1S208_104V6

Mitochondrion

nad3

230

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	△56-10	The Nptii Gene Cassette	Restriction sites - Ecorv	Knockout	Slightly reduced growth of the protonemata	Protonema	3 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20837503
NA	△56-22	The Nptii Gene Cassette	Restriction sites - Ecorv	Knockout	Slightly reduced growth of the protonemata	Protonema	3 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20837503
NA	WT	Control	Control	Control	Normal	Protonema	3 days	Direct Sequencing of PCR Products	100.00%	Complete	None	20837503
NA	56comp #1	Complemented with 56comp #1	Introduced the wild-type PpPPR_56 into PpPPR_56 KO moss line (Δ56–10). A 3× HA epitope tag sequence was inserted in front of the first PPR motif to not affect the editing function of the C-terminal DY	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	97.70%	High	Restored	32348368
NA	56comp #6	Complemented with 56comp #6	Introduced the wild-type PpPPR_56 into PpPPR_56 KO moss line (Δ56–10). A 3× HA epitope tag sequence was inserted in front of the first PPR motif to not affect the editing function of the C-terminal DY	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	93.80%	High	Restored	32348368
NA	56nad3L variant #2	Complemented with 56nad3L variant #2	Introduced 56nad3L variant of the wild-type PpPPR_56 into PpPPR_56 KO moss line (Δ56–10). A 3× HA epitope tag sequence was inserted in front of the first PPR motif to not affect the editing function	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	2.80%	Poor	NA	32348368
NA	56nad3L variant #9	Complemented with 56nad3L variant #9	Introduced 56nad3L variant of the wild-type PpPPR_56 into PpPPR_56 KO moss line (Δ56–10). A 3× HA epitope tag sequence was inserted in front of the first PPR motif to not affect the editing function	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	4.10%	Poor	NA	32348368
NA	10L[ND] #2	Complemented with 10L[ND] #2	PpPPR_56 KO mosses complemented with variant 10L[ND]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	25.00%	Low	Decreased	32348368
NA	10L[ND] #4	Complemented with 10L[ND] #4	PpPPR_56 KO mosses complemented with variant 10L[ND]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	80.00%	High	Decreased	32348368
NA	10L[LS] #3	Complemented with 10L[LS] #3	PpPPR_56 KO mosses complemented with variant 10L[LS]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	95.00%	High	Restored	32348368
NA	13L[ND] #1	Complemented with 13L[ND] #1	PpPPR_56 KO mosses complemented with variant 13L[ND]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	60.00%	High	Decreased	32348368
NA	13L[ND] #2	Complemented with 13L[ND] #2	PpPPR_56 KO mosses complemented with variant 13L[ND]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	25.00%	Low	Decreased	32348368
NA	13L[VS] #1	Complemented with 13L[VS] #1	PpPPR_56 KO mosses complemented with variant 13L[VS]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	100.00%	Complete	Restored	32348368
NA	1L[NN] #2	Complemented with 1L[NN] #2	PpPPR_56 KO mosses complemented with variant 1L[NN]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	44.00%	Medium	Decreased	32348368
NA	1L[NN] #3	Complemented with 1L[NN] #3	PpPPR_56 KO mosses complemented with variant 1L[NN]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	65.00%	High	Decreased	32348368
NA	4L[TD] #1	Complemented with 4L[TD] #1	PpPPR_56 KO mosses complemented with variant 4L[TD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	85.00%	High	NA	32348368
NA	4L[TD] #3	Complemented with 4L[TD] #3	PpPPR_56 KO mosses complemented with variant 4L[TD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	85.00%	High	NA	32348368
NA	7L[TN] #1	Complemented with 7L[TN] #1	PpPPR_56 KO mosses complemented with variant 7L[TN]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	88.00%	High	NA	32348368
NA	7L[TN] #2	Complemented with 7L[TN] #2	PpPPR_56 KO mosses complemented with variant 7L[TN]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	76.00%	High	Decreased	32348368
NA	10L[NS] #4	Complemented with 10L[NS] #4	PpPPR_56 KO mosses complemented with variant 10L[NS]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	9.00%	Poor	Decreased	32348368
NA	10L[NS] #8	Complemented with 10L[NS] #8	PpPPR_56 KO mosses complemented with variant 10L[NS]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	20.00%	Low	Decreased	32348368
NA	13L[NS] #13	Complemented with 13L[NS] #13	PpPPR_56 KO mosses complemented with variant 13L[NS]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	65.00%	High	Decreased	32348368
NA	13L[NS] #30	Complemented with 13L[NS] #30	PpPPR_56 KO mosses complemented with variant 13L[NS]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	56.00%	Medium	Decreased	32348368
NA	1L4L7L #1	Complemented with 1L4L7L #1	PpPPR_56 KO mosses complemented with variant 1L4L7L	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	72.00%	High	Decreased	32348368
NA	1L4L7L #2	Complemented with 1L4L7L #2	PpPPR_56 KO mosses complemented with variant 1L4L7L	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	50.00%	Medium	Decreased	32348368
NA	10L13L #31	Complemented with 10L13L #31	PpPPR_56 KO mosses complemented with variant 10L13L	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	5.00%	Poor	Decreased	32348368
NA	10L13L #47	Complemented with 10L13L #47	PpPPR_56 KO mosses complemented with variant 10L13L	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	4.00%	Poor	Decreased	32348368
NA	56comp 10[LD] #1	Complemented with 56comp 10[LD] #1	PpPPR_56 KO mosses complemented with 56comp 10[LD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	98.00%	High	Restored	32348368
NA	10L[VD] #5	Complemented with 10L[VD] #5	PpPPR_56 KO mosses complemented with 10L[VD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	98.00%	High	Restored	32348368
NA	10L[VD] #8	Complemented with 10L[VD] #8	PpPPR_56 KO mosses complemented with 10L[VD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	91.00%	High	Restored	32348368
NA	10L[MD] #1	Complemented with 10L[MD] #1	PpPPR_56 KO mosses complemented with 10L[MD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	95.00%	High	Restored	32348368
NA	10L[MD] #7	Complemented with 10L[MD] #7	PpPPR_56 KO mosses complemented with 10L[MD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	94.00%	High	Restored	32348368
NA	10L[PD] #1	Complemented with 10L[PD] #1	PpPPR_56 KO mosses complemented with 10L[PD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	58.00%	Medium	Decreased	32348368
NA	10L[PD] #3	Complemented with 10L[PD] #3	PpPPR_56 KO mosses complemented with 10L[PD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	46.00%	Medium	Decreased	32348368
NA	10L[SD] #2	Complemented with 10L[SD] #2	PpPPR_56 KO mosses complemented with 10L[SD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	56.00%	Medium	Decreased	32348368
NA	10L[SD] #3	Complemented with 10L[SD] #3	PpPPR_56 KO mosses complemented with 10L[SD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	70.00%	High	Decreased	32348368
NA	10L[TD] #7	Complemented with 10L[TD] #7	PpPPR_56 KO mosses complemented with 10L[TD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	88.00%	High	NA	32348368
NA	10L[TD] #8	Complemented with 10L[TD] #8	PpPPR_56 KO mosses complemented with 10L[TD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	86.00%	High	NA	32348368
NA	10L[QD] #3	Complemented with 10L[QD] #3	PpPPR_56 KO mosses complemented with 10L[QD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	93.00%	High	Restored	32348368
NA	10L[QD] #24	Complemented with 10L[QD] #24	PpPPR_56 KO mosses complemented with 10L[QD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	83.00%	High	NA	32348368
NA	10L[KD] #1	Complemented with 10L[KD] #1	PpPPR_56 KO mosses complemented with 10L[KD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	55.00%	Medium	Decreased	32348368
NA	10L[KD] #2	Complemented with 10L[KD] #2	PpPPR_56 KO mosses complemented with 10L[KD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	70.00%	High	Decreased	32348368
NA	10L[ED] #4	Complemented with 10L[ED] #4	PpPPR_56 KO mosses complemented with 10L[ED]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	8.00%	Poor	Decreased	32348368
NA	10L[ED] #7	Complemented with 10L[ED] #7	PpPPR_56 KO mosses complemented with 10L[ED]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	4.00%	Poor	Decreased	32348368
NA	56comp 13[VD] #1	Complemented with 56comp 13[VD] #1	PpPPR_56 KO mosses complemented with 56comp 13[VD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	98.00%	High	Restored	32348368
NA	13L[LD] #1	Complemented with 13L[LD] #1	PpPPR_56 KO mosses complemented with 13L[LD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	98.00%	High	Restored	32348368
NA	13L[LD] #2	Complemented with 13L[LD] #2	PpPPR_56 KO mosses complemented with 13L[LD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	88.00%	High	NA	32348368
NA	13L[MD] #2	Complemented with 13L[MD] #2	PpPPR_56 KO mosses complemented with 13L[MD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	80.00%	High	Decreased	32348368
NA	13L[MD] #15	Complemented with 13L[MD] #15	PpPPR_56 KO mosses complemented with 13L[MD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	68.00%	High	Decreased	32348368
NA	13L[PD] #8	Complemented with 13L[PD] #8	PpPPR_56 KO mosses complemented with 13L[PD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	3.00%	Poor	Decreased	32348368
NA	13L[PD] #9	Complemented with 13L[PD] #9	PpPPR_56 KO mosses complemented with 13L[PD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	3.00%	Poor	Decreased	32348368
NA	13L[SD] #1	Complemented with 13L[SD] #1	PpPPR_56 KO mosses complemented with 13L[SD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	3.00%	Poor	Decreased	32348368
NA	13L[SD] #2	Complemented with 13L[SD] #2	PpPPR_56 KO mosses complemented with 13L[SD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	3.00%	Poor	Decreased	32348368
NA	13L[TD] #2	Complemented with 13L[TD] #2	PpPPR_56 KO mosses complemented with 13L[TD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	88.00%	High	NA	32348368
NA	13L[TD] #3	Complemented with 13L[TD] #3	PpPPR_56 KO mosses complemented with 13L[TD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	95.00%	High	Restored	32348368
NA	13L[QD] #1	Complemented with 13L[QD] #1	PpPPR_56 KO mosses complemented with 13L[QD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	13.00%	Poor	Decreased	32348368
NA	13L[QD] #6	Complemented with 13L[QD] #6	PpPPR_56 KO mosses complemented with 13L[QD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	18.00%	Poor	Decreased	32348368
NA	13L[KD] #2	Complemented with 13L[KD] #2	PpPPR_56 KO mosses complemented with 13L[KD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	75.00%	High	Decreased	32348368
NA	13L[KD] #3	Complemented with 13L[KD] #3	PpPPR_56 KO mosses complemented with 13L[KD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	60.00%	High	Decreased	32348368
NA	13L[ED] #1	Complemented with 13L[ED] #1	PpPPR_56 KO mosses complemented with 13L[ED]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	3.00%	Poor	Decreased	32348368
NA	13L[ED] #2	Complemented with 13L[ED] #2	PpPPR_56 KO mosses complemented with 13L[ED]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	4.00%	Poor	Decreased	32348368

Physcomitrium patens

Mitochondrion

nad4

272

CDS

C-to-U

NA=>NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	WT	Wild Type	Wild Type	No mutant	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	100.00%	Complete	None	29718364
NA	△56-22	Knockout (KO) line	Procedures to obtain moss lines PpPPR_56 KO (Δ56-22) and PpPPR_71 KO (Δ71 6-11) have been previously described (Ohtani et al. 2010, Tasaki et al. 2010).	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56FL	Complementation	Full-length (FL) version of PpPPR_56 was transformed into the respective KO moss background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	100.00%	Complete	Restored	29718364
NA	56ΔE/DYW	Complementation	Truncated version (ΔE/DYW) of PpPPR_56 was transformed into the respective KO moss background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56△DYW	Complementation	Truncated version (ΔDYW) of PpPPR_56 was transformed into the respective KO moss background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56△E	Complementation	Truncated version (ΔE) of PpPPR_56 was transformed into the respective KO moss background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR+71E/71DYW	Complementation	Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR+71E/56DYW	Complementation	Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR+56E/71DYW	Complementation	Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR+56E/56DYW	Complementation	Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	100.00%	Complete	Restored	29718364
NA	56PPR+56E/45DYW	Complementation	Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	95.00%	High	Restored	29718364
NA	56PPR+56E/65DYW	Complementation	Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR+56E/77DYW	Complementation	Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR+56E/78DYW	Complementation	Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR+56E/79DYW	Complementation	Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR+56E/91DYW	Complementation	Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR+56E/98DYW	Complementation	Two editing mutants were transformed by various constructs obtained by swapping from the E and/or DYW domains of PpPPR_56 or PpPPR_71 to the cognate region of another P. patens editing factor.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56M1	Complementation	To investigate their roles in RNA editing, we generated transgenic lines with mutated DYW domains in which the HxExnCxxC signature was changed to AxAxnCxxC (56M1 and 71M1) and HxExnAxxA (56M2 and 71M2	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56M2	Complementation	To investigate their roles in RNA editing, we generated transgenic lines with mutated DYW domains in which the HxExnCxxC signature was changed to AxAxnCxxC (56M1 and 71M1) and HxExnAxxA (56M2 and 71M2	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56M3	Complementation	To investigate their roles in RNA editing, we generated transgenic lines with mutated DYW domains in which the HxExnCxxC signature was changed to AxAxnCxxC (56M1 and 71M1) and HxExnAxxA (56M2 and 71M2	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR-E-56DYW	Complementation	RNA editing of nad3-C230 and nad4-C272 in various transgenic lines harboring chimeric PpPPR_56 constructs (56PPE-E-r71dywA, -dywB or -dywC) in a PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	100.00%	Complete	Restored	29718364
NA	56PPR-E-71DYW	Complementation	RNA editing of nad3-C230 and nad4-C272 in various transgenic lines harboring chimeric PpPPR_56 constructs (56PPE-E-r71dywA, -dywB or -dywC) in a PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR-E-r71dywA	Complementation	RNA editing of nad3-C230 and nad4-C272 in various transgenic lines harboring chimeric PpPPR_56 constructs (56PPE-E-r71dywA, -dywB or -dywC) in a PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	90.00%	High	Restored	29718364
NA	56PPR-E-r71dywB	Complementation	RNA editing of nad3-C230 and nad4-C272 in various transgenic lines harboring chimeric PpPPR_56 constructs (56PPE-E-r71dywA, -dywB or -dywC) in a PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	50.00%	Medium	Restored	29718364
NA	56PPR-E-r71dywC	Complementation	RNA editing of nad3-C230 and nad4-C272 in various transgenic lines harboring chimeric PpPPR_56 constructs (56PPE-E-r71dywA, -dywB or -dywC) in a PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	100.00%	Complete	Restored	29718364
NA	56PPR-E-r71dywB-1	Complementation	RNA editing in Δ56 complemented lines with dywB-1 and dywB-2, respectively.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	45.00%	Medium	Restored	29718364
NA	56PPR-E-r71dywB-2	Complementation	RNA editing in Δ56 complemented lines with dywB-1 and dywB-2, respectively.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	100.00%	Complete	Restored	29718364
NA	56PPR-E+71DYW-m1	Complementation	RNA editing efficiency of nad3-C230 and nad4-C272 in Δ56 complemented lines with 56PPR-E + 71DYW-m1 and -m2, respectively.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR-E+71DYW-m2	Complementation	RNA editing efficiency of nad3-C230 and nad4-C272 in Δ56 complemented lines with 56PPR-E + 71DYW-m1 and -m2, respectively.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	50.00%	Medium	Restored	29718364
NA	56PPR-E+78DYW I38M/S39H #4	Complementation	RNA editing in the PpPPR_56 KO mosses complemented with 56PPR-E + 78DYW I38M/S39H.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	35.00%	Low	Restored	29718364
NA	56PPR-E+78DYW I38M/S39H #6	Complementation	RNA editing in the PpPPR_56 KO mosses complemented with 56PPR-E + 78DYW I38M/S39H.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	40.00%	Medium	Restored	29718364
NA	56PPR-E+71DYW A23D	Complementation and mutation	Mutated 56PPR-E/71DYW variants were introduced in the PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR-E+71DYW S25C	Complementation and mutation	Mutated 56PPR-E/71DYW variants were introduced in the PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR-E+71DYW Y26S	Complementation and mutation	Mutated 56PPR-E/71DYW variants were introduced in the PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR-E+71DYW A34V	Complementation and mutation	Mutated 56PPR-E/71DYW variants were introduced in the PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR-E+71DYW V37L/L38M	Complementation and mutation	Mutated 56PPR-E/71DYW variants were introduced in the PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR-E+71DYW S39H	Complementation and mutation	Mutated 56PPR-E/71DYW variants were introduced in the PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR-E+71DYW L40T	Complementation and mutation	Mutated 56PPR-E/71DYW variants were introduced in the PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364
NA	56PPR-E+71DYW S42P	Complementation and mutation	Mutated 56PPR-E/71DYW variants were introduced in the PpPPR_56 KO background.	Knockout	NA	Protonema	4-day-old	Direct sequencing was performed on the cDNA	0.00%	Unedited	Absent	29718364

Physcomitrium patens

PP1S208_104V6

Mitochondrion

nad4

272

CDS

C-to-U

UCA=>UUA
NA=>NA

S=>L
NA=>NA

Recoding
NA

**Experiment Details**
Genotype (Ecotype)	Allele	Treatment	Treatment Detail	Mutant Type	Phenotype	Tissue	Development Stage	Detection Method	Editing Frequency	Editing Extent	Mutant Effect	PMID
NA	△56-10	The Nptii Gene Cassette	Restriction sites - Ecorv	Knockout	Slightly reduced growth of the protonemata	Protonema	3 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20837503
NA	△56-22	The Nptii Gene Cassette	Restriction sites - Ecorv	Knockout	Slightly reduced growth of the protonemata	Protonema	3 days	Direct Sequencing of PCR Products	0.00%	Unedited	Absent	20837503
NA	WT	Control	Control	Control	Normal	Protonema	3 days	Direct Sequencing of PCR Products	100.00%	Complete	None	20837503
NA	56comp #1	Complemented with 56comp #1	Introduced the wild-type PpPPR_56 into PpPPR_56 KO moss line (Δ56–10). A 3× HA epitope tag sequence was inserted in front of the first PPR motif to not affect the editing function of the C-terminal DY	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	99.30%	High	Restored	32348368
NA	56comp #6	Complemented with 56comp #6	Introduced the wild-type PpPPR_56 into PpPPR_56 KO moss line (Δ56–10). A 3× HA epitope tag sequence was inserted in front of the first PPR motif to not affect the editing function of the C-terminal DY	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	97.30%	High	Restored	32348368
NA	56nad3L variant #2	Complemented with 56nad3L variant #2	Introduced 56nad3L variant of the wild-type PpPPR_56 into PpPPR_56 KO moss line (Δ56–10). A 3× HA epitope tag sequence was inserted in front of the first PPR motif to not affect the editing function	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	3.70%	Poor	NA	32348368
NA	56nad3L variant #9	Complemented with 56nad3L variant #9	Introduced 56nad3L variant of the wild-type PpPPR_56 into PpPPR_56 KO moss line (Δ56–10). A 3× HA epitope tag sequence was inserted in front of the first PPR motif to not affect the editing function	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	3.50%	Poor	NA	32348368
NA	10L[ND] #2	Complemented with 10L[ND] #2	PpPPR_56 KO mosses complemented with variant 10L[ND]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	70.00%	High	Decreased	32348368
NA	10L[ND] #4	Complemented with 10L[ND] #4	PpPPR_56 KO mosses complemented with variant 10L[ND]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	95.00%	High	Restored	32348368
NA	10L[LS] #3	Complemented with 10L[LS} #3	PpPPR_56 KO mosses complemented with variant 10L[LS]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	100.00%	Complete	Restored	32348368
NA	13L[ND] #1	Complemented with 13L[ND] #1	PpPPR_56 KO mosses complemented with variant 13L[ND]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	85.00%	High	NA	32348368
NA	13L[ND] #2	Complemented with 13L[ND] #2	PpPPR_56 KO mosses complemented with variant 13L[ND]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	60.00%	High	Decreased	32348368
NA	13L[VS] #1	Complemented with 13L[VS] #1	PpPPR_56 KO mosses complemented with variant 13L[VS]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	100.00%	Complete	Restored	32348368
NA	1L[NN] #2	Complemented with 1L[NN] #2	PpPPR_56 KO mosses complemented with variant 1L[NN]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	74.00%	High	Decreased	32348368
NA	1L[NN] #3	Complemented with 1L[NN] #3	PpPPR_56 KO mosses complemented with variant 1L[NN]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	85.00%	High	NA	32348368
NA	4L[TD] #1	Complemented with 4L[TD] #1	PpPPR_56 KO mosses complemented with variant 4L[TD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	90.00%	High	Restored	32348368
NA	4L[TD] #3	Complemented with 4L[TD] #3	PpPPR_56 KO mosses complemented with variant 4L[TD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	95.00%	High	Restored	32348368
NA	7L[TN] #1	Complemented with 7L[TN] #1	PpPPR_56 KO mosses complemented with variant 7L[TN]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	93.00%	High	Restored	32348368
NA	7L[TN] #2	Complemented with 7L[TN] #2	PpPPR_56 KO mosses complemented with variant 7L[TN]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	80.00%	High	Decreased	32348368
NA	10L[NS] #4	Complemented with 10L[NS] #4	PpPPR_56 KO mosses complemented with variant 10L[NS]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	48.00%	Medium	Decreased	32348368
NA	10L[NS] #8	Complemented with 10L[NS] #8	PpPPR_56 KO mosses complemented with variant 10L[NS]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	71.00%	High	Decreased	32348368
NA	13L[NS] #13	Complemented with 13L[NS] #13	PpPPR_56 KO mosses complemented with variant 13L[NS]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	86.00%	High	NA	32348368
NA	13L[NS] #30	Complemented with 13L[NS] #30	PpPPR_56 KO mosses complemented with variant 13L[NS]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	80.00%	High	Decreased	32348368
NA	1L4L7L #1	Complemented with 1L4L7L #1	PpPPR_56 KO mosses complemented with variant 1L4L7L	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	81.00%	High	NA	32348368
NA	1L4L7L #2	Complemented with 1L4L7L #2	PpPPR_56 KO mosses complemented with variant 1L4L7L	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	56.00%	Medium	Decreased	32348368
NA	10L13L #31	Complemented with 10L13L #31	PpPPR_56 KO mosses complemented with variant 10L13L	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	5.00%	Poor	Decreased	32348368
NA	10L13L #47	Complemented with 10L13L #47	PpPPR_56 KO mosses complemented with variant 10L13L	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	3.00%	Poor	Decreased	32348368
NA	56comp 10[LD] #1	Complemented with 56comp 10[LD] #1	PpPPR_56 KO mosses complemented with 56comp 10[LD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	100.00%	Complete	Restored	32348368
NA	10L[VD] #5	Complemented with 10L[VD] #5	PpPPR_56 KO mosses complemented with 10L[VD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	100.00%	Complete	Restored	32348368
NA	10L[VD] #8	Complemented with 10L[VD] #8	PpPPR_56 KO mosses complemented with 10L[VD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	99.00%	High	Restored	32348368
NA	10L[MD] #1	Complemented with 10L[MD] #1	PpPPR_56 KO mosses complemented with 10L[MD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	98.00%	High	Restored	32348368
NA	10L[MD] #7	Complemented with 10L[MD] #7	PpPPR_56 KO mosses complemented with 10L[MD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	99.00%	High	Restored	32348368
NA	10L[PD] #1	Complemented with 10L[PD] #1	PpPPR_56 KO mosses complemented with 10L[PD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	85.00%	High	NA	32348368
NA	10L[PD] #3	Complemented with 10L[PD] #3	PpPPR_56 KO mosses complemented with 10L[PD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	84.00%	High	NA	32348368
NA	10L[SD] #2	Complemented with 10L[SD] #2	PpPPR_56 KO mosses complemented with 10L[SD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	98.00%	High	Restored	32348368
NA	10L[SD] #3	Complemented with 10L[SD] #3	PpPPR_56 KO mosses complemented with 10L[SD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	99.00%	High	Restored	32348368
NA	10L[TD] #7	Complemented with 10L[TD] #7	PpPPR_56 KO mosses complemented with 10L[TD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	100.00%	Complete	Restored	32348368
NA	10L[TD] #8	Complemented with 10L[TD] #8	PpPPR_56 KO mosses complemented with 10L[TD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	98.00%	High	Restored	32348368
NA	10L[QD] #3	Complemented with 10L[QD] #3	PpPPR_56 KO mosses complemented with 10L[QD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	99.00%	High	Restored	32348368
NA	10L[QD] #24	Complemented with 10L[QD] #24	PpPPR_56 KO mosses complemented with 10L[QD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	99.00%	High	Restored	32348368
NA	10L[KD] #1	Complemented with 10L[KD] #1	PpPPR_56 KO mosses complemented with 10L[KD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	88.00%	High	NA	32348368
NA	10L[KD] #2	Complemented with 10L[KD] #2	PpPPR_56 KO mosses complemented with 10L[KD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	90.00%	High	Restored	32348368
NA	10L[ED] #4	Complemented with 10L[ED] #4	PpPPR_56 KO mosses complemented with 10L[ED]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	44.00%	Medium	Decreased	32348368
NA	10L[ED] #7	Complemented with 10L[ED] #7	PpPPR_56 KO mosses complemented with 10L[ED]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	18.00%	Poor	Decreased	32348368
NA	56comp 13[VD] #1	Complemented with 56comp 13[VD] #1	PpPPR_56 KO mosses complemented with 56comp 13[VD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	99.00%	High	Restored	32348368
NA	13L[LD] #1	Complemented with 13L[LD] #1	PpPPR_56 KO mosses complemented with 13L[LD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	99.00%	High	Restored	32348368
NA	13L[LD] #2	Complemented with 13L[LD] #2	PpPPR_56 KO mosses complemented with 13L[LD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	100.00%	Complete	NA	32348368
NA	13L[MD] #2	Complemented with 13L[MD] #2	PpPPR_56 KO mosses complemented with 13L[MD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	93.00%	High	Restored	32348368
NA	13L[MD] #15	Complemented with 13L[MD] #15	PpPPR_56 KO mosses complemented with 13L[MD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	88.00%	High	NA	32348368
NA	13L[PD] #8	Complemented with 13L[PD] #8	PpPPR_56 KO mosses complemented with 13L[PD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	3.00%	Poor	Decreased	32348368
NA	13L[PD] #9	Complemented with 13L[PD] #9	PpPPR_56 KO mosses complemented with 13L[PD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	0.00%	Unedited	Absent	32348368
NA	13L[SD] #1	Complemented with 13L[SD] #1	PpPPR_56 KO mosses complemented with 13L[SD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	6.00%	Poor	Decreased	32348368
NA	13L[SD] #2	Complemented with 13L[SD] #2	PpPPR_56 KO mosses complemented with 13L[SD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	14.00%	Poor	Decreased	32348368
NA	13L[TD] #2	Complemented with 13L[TD] #2	PpPPR_56 KO mosses complemented with 13L[TD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	95.00%	High	Restored	32348368
NA	13L[TD] #3	Complemented with 13L[TD] #3	PpPPR_56 KO mosses complemented with 13L[TD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	99.00%	High	Restored	32348368
NA	13L[QD] #1	Complemented with 13L[QD] #1	PpPPR_56 KO mosses complemented with 13L[QD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	31.00%	Low	Decreased	32348368
NA	13L[QD] #6	Complemented with 13L[QD] #6	PpPPR_56 KO mosses complemented with 13L[QD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	50.00%	Medium	Decreased	32348368
NA	13L[KD] #2	Complemented with 13L[KD] #2	PpPPR_56 KO mosses complemented with 13L[KD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	85.00%	High	NA	32348368
NA	13L[KD] #3	Complemented with 13L[KD] #3	PpPPR_56 KO mosses complemented with 13L[KD]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	83.00%	High	NA	32348368
NA	13L[ED] #1	Complemented with 13L[ED] #1	PpPPR_56 KO mosses complemented with 13L[ED]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	5.00%	Poor	Decreased	32348368
NA	13L[ED] #2	Complemented with 13L[ED] #2	PpPPR_56 KO mosses complemented with 13L[ED]	Knockout	NA	Protonema	4-day-old	RT-PCR, cDNA sequencing and chromatographs were analyzed usi	7.00%	Poor	Decreased	32348368

Editing Factor:	PpPPR_56
Synonym:	Phypa_169414
Description:	Encoding a PPR-DYW protein involved in RNA editing; This assay showed that both E and DYW domains are required for RNA editing at the target sites, and that the conserved zinc-binding signature and the terminal triplet of the DYW domain are essential for editing; In addition, DYW domain-swapping experiments demonstrated that DYW domains are functionally different between PpPPR_56 and other mitochondrial PPR editing factors, and that residues 37-42 of the DYW domain are involved in site-specific editing
Protein Family:	PPR
Subclass:	DYW
Construct Structure:	PLS-E/E+-DYW, PLS-E-DYW
Gene ID & Species:	NA (Physcomitrium patens) PP1S208_104V6 (Physcomitrium patens)
Edited Gene(s):	nad3 nad4
Editing Type(s):	C-to-U (200)
Publication(s):	[1] The L motifs of two moss pentatricopeptide repeat proteins are involved in RNA editing but predominantly not in RNA recognition., PloS one, 2020. [PMID=32348368] [2] The DYW Domains of Pentatricopeptide Repeat RNA Editing Factors Contribute to Discriminate Target and Non-Target Editing Sites., Plant & cell physiology, 2018. [PMID=29718364] [3] Targeted gene disruption identifies three PPR-DYW proteins involved in RNA editing for five editing sites of the moss mitochondrial transcripts., Plant & cell physiology, 2010. [PMID=20837503]

Plant Editosome Database
a curated database of RNA editosome in plants

Summary

Editing Details