Introduction

Hodgkin lymphoma (HL) is a type of B-cell lymphoma. To diagnose the subtypes, biopsies are taken and immunostained. The slides are scanned to produce high-resolution digital whole slide images (WSI). Pathologists manually inspect the spatial distribution of cells, but little is known on the statistical properties of cell distributions in WSIs. Such properties would give valuable information for the construction of theoretical models that describe the invasion of malignant cells in the lymph node and the intercellular interactions.In this work, we define and discuss HL cell graphs. We identify CD30(+) cells in HL WSIs, bringing together the fields of digital imaging and network analysis. We define special graphs based on the positions of the immunostained cells. We present an automatic analysis of complete WSIs to determine significant morphological and immunohistochemical features of HL cells and their spatial distribution in the lymph node tissue under three different medical conditions: lymphadenitis (LA) and two types of HL. We analyze the vertex degree distributions of CD30 cell graphs and compare them to a null model. CD30 cell graphs show higher vertex degrees than expected by a random unit disk graph, suggesting clustering of the cells. We found that a gamma distribution is suitable to model the vertex degree distributions of CD30 cell graphs, meaning that they are not scale-free. Moreover, we compare the graphs for LA and two subtypes of HL. LA and classical HL showed different vertex degree distributions. The vertex degree distributions of the two HL subtypes NScHL and mixed cellularity HL (MXcHL) were similar.The CellProfiler pipeline used for cell detection is available at https://sourceforge.net/projects/cellgraphs/.ina.koch@bioinformatik.uni-frankfurt.deSupplementary data are available at Bioinformatics online.

Publications

  1. CD30 cell graphs of Hodgkin lymphoma are not scale-free--an image analysis approach.
    Cite this
    Schäfer H, Schäfer T, Ackermann J, Dichter N, Döring C, Hartmann S, Hansmann ML, Koch I, 2016-01-01 - Bioinformatics (Oxford, England)

Credits

  1. Hendrik Schäfer
    Developer

    Department of Molecular Bioinformatics, Institute of Computer Science

  2. Tim Schäfer
    Developer

    Department of Molecular Bioinformatics, Institute of Computer Science

  3. Jörg Ackermann
    Developer

    Department of Molecular Bioinformatics, Institute of Computer Science

  4. Norbert Dichter
    Developer

    Department of Molecular Bioinformatics, Institute of Computer Science

  5. Claudia Döring
    Developer

    Dr. Senckenbergisches Institut für Pathologie, Universitätsklinikum Frankfurt, Germany

  6. Sylvia Hartmann
    Developer

    Dr. Senckenbergisches Institut für Pathologie, Universitätsklinikum Frankfurt, Germany

  7. Martin-Leo Hansmann
    Developer

    Dr. Senckenbergisches Institut für Pathologie, Universitätsklinikum Frankfurt, Germany

  8. Ina Koch
    Investigator

    Department of Molecular Bioinformatics, Institute of Computer Science

Community Ratings

UsabilityEfficiencyReliabilityRated By
0 user
Sign in to rate
Summary
AccessionBT000157
Tool TypeApplication
Category
PlatformsLinux/Unix
Technologies
User InterfaceTerminal Command Line
Download Count0
Submitted ByIna Koch