Introduction

Flow cytometric analysis allows rapid single cell interrogation of surface and intracellular determinants by measuring fluorescence intensity of fluorophore-conjugated reagents. The availability of new platforms, allowing detection of increasing numbers of cell surface markers, has challenged the traditional technique of identifying cell populations by manual gating and resulted in a growing need for the development of automated, high-dimensional analytical methods. We present a direct multivariate finite mixture modeling approach, using skew and heavy-tailed distributions, to address the complexities of flow cytometric analysis and to deal with high-dimensional cytometric data without the need for projection or transformation. We demonstrate its ability to detect rare populations, to model robustly in the presence of outliers and skew, and to perform the critical task of matching cell populations across samples that enables downstream analysis. This advance will facilitate the application of flow cytometry to new, complex biological and clinical problems.

Publications

  1. Automated high-dimensional flow cytometric data analysis.
    Cite this
    Pyne S, Hu X, Wang K, Rossin E, Lin TI, Maier LM, Baecher-Allan C, McLachlan GJ, Tamayo P, Hafler DA, De Jager PL, Mesirov JP, 2009-05-01 - Proceedings of the National Academy of Sciences of the United States of America

Credits

  1. Saumyadipta Pyne
    Developer

    -, Broad Institute of MIT and Harvard, United States of America

  2. Xinli Hu
    Developer

  3. Kui Wang
    Developer

  4. Elizabeth Rossin
    Developer

  5. Tsung-I Lin
    Developer

  6. Lisa M Maier
    Developer

  7. Clare Baecher-Allan
    Developer

  8. Geoffrey J McLachlan
    Developer

  9. Pablo Tamayo
    Developer

  10. David A Hafler
    Developer

  11. Philip L De Jager
    Developer

  12. Jill P Mesirov
    Investigator

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Summary
AccessionBT001713
Tool TypeApplication
Category
PlatformsLinux/Unix
TechnologiesR
User InterfaceTerminal Command Line
Download Count0
Submitted ByJill P Mesirov