Introduction

A major challenge for mass spectrometric-based lipidomics, aiming at describing all lipid species in a biological sample, lies in the computational and bioinformatic processing of the large amount of data that arises after data acquisition. Lipid-Pro is a software tool that supports the identification of lipids by interpreting large datasets generated by liquid chromatography--tandem mass spectrometry (LC-MS/MS) using the advanced data-independent acquisition mode MS(E). In the MS(E) mode, the instrument fragments all molecular ions generated from a sample and records time-resolved molecular ion data as well as fragment ion data for every detectable molecular ion. Lipid-Pro matches the retention time-aligned mass-to-charge ratio data of molecular- and fragment ions with a lipid database and generates a report on all identified lipid species. For generation of the lipid database, Lipid-Pro provides a module for construction of lipid species and their fragments using a flexible building block approach. Hence, Lipid-Pro is an easy to use analysis tool to interpret complex MS(E) lipidomics data and also offers a module to generate a user-specific lipid database.Lipid-Pro is freely available at: http://www.neurogenetics.biozentrum.uni-wuerzburg.de/en/project/services/lipidpro/.Supplementary data are available at Bioinformatics online.

Publications

  1. Lipid-Pro: a computational lipid identification solution for untargeted lipidomics on data-independent acquisition tandem mass spectrometry platforms.
    Cite this
    Ahmed Z, Mayr M, Zeeshan S, Dandekar T, Mueller MJ, Fekete A, 2015-04-01 - Bioinformatics (Oxford, England)

Credits

  1. Zeeshan Ahmed
    Developer

    Department of Neurobiology and Genetics, Biocenter, Germany

  2. Michel Mayr
    Developer

    Department of Neurobiology and Genetics, Biocenter, Germany

  3. Saman Zeeshan
    Developer

    Department of Neurobiology and Genetics, Biocenter, Germany

  4. Thomas Dandekar
    Developer

    Department of Neurobiology and Genetics, Biocenter, Germany

  5. Martin J Mueller
    Developer

    Department of Neurobiology and Genetics, Biocenter, Germany

  6. Agnes Fekete
    Investigator

    Department of Neurobiology and Genetics, Biocenter, Germany

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Summary
AccessionBT002860
Tool TypeApplication
Category
PlatformsWindows
Technologies
User InterfaceTerminal Command Line
Download Count0
Country/RegionGermany
Submitted ByAgnes Fekete