Introduction

Accurate protein identification and quantitation are critical when interpreting the biological relevance of large-scale shotgun proteomics data sets. Although significant technical advances in peptide and protein identification have been made, accurate quantitation of high-throughput data sets remains a key challenge in mass spectrometry data analysis and is a labor intensive process for many proteomics laboratories. Here, we report a new SILAC-based proteomics quantitation software tool, named IsoQuant, which is used to process high mass accuracy mass spectrometry data. IsoQuant offers a convenient quantitation framework to calculate peptide/protein relative abundance ratios. At the same time, it also includes a visualization platform that permits users to validate the quality of SILAC peptide and protein ratios. The program is written in the C# programming language under the Microsoft .NET framework version 4.0 and has been tested to be compatible with both 32-bit and 64-bit Windows 7. It is freely available to noncommercial users at http://www.proteomeumb.org/MZw.html .

Publications

  1. IsoQuant: a software tool for stable isotope labeling by amino acids in cell culture-based mass spectrometry quantitation.
    Cite this
    Liao Z, Wan Y, Thomas SN, Yang AJ, 2012-05-01 - Analytical chemistry

Credits

  1. Zhongping Liao
    Developer

    Greenebaum Cancer Center, University of Maryland, United States of America

  2. Yunhu Wan
    Developer

  3. Stefani N Thomas
    Developer

  4. Austin J Yang
    Investigator

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Summary
AccessionBT004446
Tool TypeApplication
Category
PlatformsWindows
Technologies
User InterfaceTerminal Command Line
Download Count0
Submitted ByAustin J Yang